Search Results - "Creutzburg, Sjoerd C.A."

A growth‐ and bioluminescence‐based bioreporter for the in vivo detection of novel biocatalysts by Rossum, Teunke, Muras, Aleksandra, Baur, Marco J.J., Creutzburg, Sjoerd C.A., Oost, John, Kengen, Servé W.M.

“…Summary The use of bioreporters in high‐throughput screening for small molecules is generally laborious and/or expensive. The technology can be simplified by…”
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The miniature CRISPR-Cas12m effector binds DNA to block transcription by Wu, Wen Y., Mohanraju, Prarthana, Liao, Chunyu, Adiego-Pérez, Belén, Creutzburg, Sjoerd C.A., Makarova, Kira S., Keessen, Karlijn, Lindeboom, Timon A., Khan, Tahseen S., Prinsen, Stijn, Joosten, Rob, Yan, Winston X., Migur, Anzhela, Laffeber, Charlie, Scott, David A., Lebbink, Joyce H.G., Koonin, Eugene V., Beisel, Chase L., van der Oost, John

“…CRISPR-Cas are prokaryotic adaptive immune systems. Cas nucleases generally use CRISPR-derived RNA guides to specifically bind and cleave DNA or RNA targets…”
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Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms by Claassens, Nico J, Siliakus, Melvin F, Spaans, Sebastiaan K, Creutzburg, Sjoerd C A, Nijsse, Bart, Schaap, Peter J, Quax, Tessa E F, van der Oost, John

“…High-level, recombinant production of membrane-integrated proteins in Escherichia coli is extremely relevant for many purposes, but has also been proven…”
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Good guide, bad guide: spacer sequence-dependent cleavage efficiency of Cas12a by Creutzburg, Sjoerd C A, Wu, Wen Y, Mohanraju, Prarthana, Swartjes, Thomas, Alkan, Ferhat, Gorodkin, Jan, Staals, Raymond H J, van der Oost, John

“…Abstract Genome editing has recently made a revolutionary development with the introduction of the CRISPR–Cas technology. The programmable CRISPR-associated…”
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Medium-throughput in vitro detection of DNA cleavage by CRISPR-Cas12a by Creutzburg, Sjoerd C.A., Swartjes, Thomas, van der Oost, John

“…•Medium-throughput method for DNA cleavage quantification.•Sensitive quantification of DNA cleavage by CRISPR-Cas.•No need for expensive equipment. Quantifying…”
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Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas by Patinios, Constantinos, Creutzburg, Sjoerd C A, Arifah, Adini Q, Adiego-Pérez, Belén, Gyimah, Evans A, Ingham, Colin J, Kengen, Servé W M, van der Oost, John, Staals, Raymond H J

“…Abstract CRISPR-Cas is a powerful tool for genome editing in bacteria. However, its efficacy is dependent on host factors (such as DNA repair pathways) and/or…”
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