Search Results - "Cremo, Christine R"

Biochemistry of smooth muscle myosin light chain kinase by Hong, Feng, Haldeman, Brian D., Jackson, Del, Carter, Mike, Baker, Jonathan E., Cremo, Christine R.

“…► We summarize biochemical work on smooth muscle MLCK pertaining to smooth muscle function. ► Focus is on discussion of new findings, areas that require…”
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Velocities of unloaded muscle filaments are not limited by drag forces imposed by myosin cross-bridges by Brizendine, Richard K., Alcala, Diego B., Carter, Michael S., Haldeman, Brian D., Facemyer, Kevin C., Baker, Josh E., Cremo, Christine R.

“…It is not known which kinetic step in the acto-myosin ATPase cycle limits contraction speed in unloaded muscles (V₀). Huxley’s 1957 model [Huxley AF (1957)Prog…”
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kinesin-1 motor protein is regulated by a direct interaction of its head and tail by Dietrich, Kristen A, Sindelar, Charles V, Brewer, Paul D, Downing, Kenneth H, Cremo, Christine R, Rice, Sarah E

“…Kinesin-1 is a molecular motor protein that transports cargo along microtubules. Inside cells, the vast majority of kinesin-1 is regulated to conserve ATP and…”
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Direct evidence for functional smooth muscle myosin II in the 10S self-inhibited monomeric conformation in airway smooth muscle cells by Milton, Deanna L., Schneck, Amy N., Ziech, Dominique A., Ba, Mariam, Facemyer, Kevin C., Halayko, Andrew J., Baker, Jonathan E., Gerthoffer, William T., Cremo, Christine R., Spudich, James A.

“…The 10S self-inhibited monomeric conformation of myosin II has been characterized extensively in vitro. Based upon its structural and functional…”
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Role of the Tail in the Regulated State of Myosin 2 by Jung, Hyun Suk, Billington, Neil, Thirumurugan, Kavitha, Salzameda, Bridget, Cremo, Christine R., Chalovich, Joseph M., Chantler, Peter D., Knight, Peter J.

“…Myosin 2 from vertebrate smooth muscle or non-muscle sources is in equilibrium between compact, inactive monomers and thick filaments under physiological…”
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Diffusion of myosin light chain kinase on actin: A mechanism to enhance myosin phosphorylation rates in smooth muscle by Hong, Feng, Brizendine, Richard K, Carter, Michael S, Alcala, Diego B, Brown, Avery E, Chattin, Amy M, Haldeman, Brian D, Walsh, Michael P, Facemyer, Kevin C, Baker, Josh E, Cremo, Christine R

“…Smooth muscle myosin (SMM) light chain kinase (MLCK) phosphorylates SMM, thereby activating the ATPase activity required for muscle contraction. The abundance…”
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Modification of Interface between Regulatory and Essential Light Chains Hampers Phosphorylation-dependent Activation of Smooth Muscle Myosin by Ni, Shaowei, Hong, Feng, Haldeman, Brian D., Baker, Josh E., Facemyer, Kevin C., Cremo, Christine R.

“…We examined the regulatory importance of interactions between regulatory light chain (RLC), essential light chain (ELC), and adjacent heavy chain (HC) in the…”
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Broad disorder and the allosteric mechanism of myosin II regulation by phosphorylation by Vileno, Bertrand, Chamoun, Jean, Liang, Hua, Brewer, Paul, Haldeman, Brian D, Facemyer, Kevin C, Salzameda, Bridget, Song, Likai, Li, Hui-Chun, Cremo, Christine R, Fajer, Piotr G

“…Double electron electron resonance EPR methods was used to measure the effects of the allosteric modulators, phosphorylation, and ATP, on the distances and…”
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Evidence for S2 flexibility by direct visualization of quantum dot-labeled myosin heads and rods within smooth muscle myosin filaments moving on actin in vitro by Brizendine, Richard K, Anuganti, Murali, Cremo, Christine R

“…Myosins in muscle assemble into filaments by interactions between the C-terminal light meromyosin (LMM) subdomains of the coiled-coil rod domain. The two head…”
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Isoform selectivities of novel 4-hydroxycoumarin imines as inhibitors of myosin II by Smith, Joshua D., Brawley, Jhonnathan, Bordenave, Kate C., Olsen, Ryan K., Intasiri, Amarawan, Cremo, Christine R., Bell, Thomas W.

“…Muscle myosin inhibition could be used to treat many medical conditions involving hypercontractile states, including muscle spasticity, chronic musculoskeletal…”
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Using the SpyTag SpyCatcher system to label smooth muscle myosin II filaments with a quantum dot on the regulatory light chain by Brizendine, Richard K., Anuganti, Murali, Cremo, Christine R.

“…The regulatory light chain (RLC) of myosin is commonly tagged to monitor myosin behavior in vitro, in muscle fibers, and in cells. The goal of this study was…”
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The N-terminal Lobes of Both Regulatory Light Chains Interact with the Tail Domain in the 10 S-inhibited Conformation of Smooth Muscle Myosin by Salzameda, Bridget, Facemyer, Kevin C., Beck, Brian W., Cremo, Christine R.

“…In the presence of ATP, unphosphorylated smooth muscle myosin can form a catalytically inactive monomer that sediments at 10 Svedbergs (10 S). The tail of 10 S…”
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Novel Sensors of the Regulatory Switch on the Regulatory Light Chain of Smooth Muscle Myosin by Mazhari, Sam M., Selser, Curtis T., Cremo, Christine R.

“…Smooth muscle myosin can be switched on by phosphorylation of Ser-19 of the regulatory light chain. Our previous photocross-linking results suggested that an…”
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The Kinetics Underlying the Velocity of Smooth Muscle Myosin Filament Sliding on Actin Filaments in Vitro by Haldeman, Brian D., Brizendine, Richard K., Facemyer, Kevin C., Baker, Josh E., Cremo, Christine R.

“…Actin-myosin interactions are well studied using soluble myosin fragments, but little is known about effects of myosin filament structure on mechanochemistry…”
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Structural Model of the Regulatory Domain of Smooth Muscle Heavy Meromyosin by Wahlstrom, Jan L, Randall, Jr, M Allen, Lawson, J David, Lyons, Derek E, Siems, William F, Crouch, Greg J, Barr, Regina, Facemyer, Kevin C, Cremo, Christine R

“…The goal of this study was to provide structural information about the regulatory domains of double-headed smooth muscle heavy meromyosin, including the N…”
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Both Heads of Tissue-derived Smooth Muscle Heavy Meromyosin Bind to Actin in the Presence of ADP by Ellison, Patricia A, DePew, Zachary S, Cremo, Christine R

“…The effect of ADP and phosphorylation upon the actin binding properties of heavy meromyosin was investigated using three fluorescence methods that monitor the…”
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A mixed-kinetic model describes unloaded velocities of smooth, skeletal, and cardiac muscle myosin filaments in vitro by Brizendine, Richard K, Sheehy, Gabriel G, Alcala, Diego B, Novenschi, Sabrina I, Baker, Josh E, Cremo, Christine R

“…In vitro motility assays, where purified myosin and actin move relative to one another, are used to better understand the mechanochemistry of the actomyosin…”
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Sucrose increases the activation energy barrier for actin–myosin strong binding by Jackson, Del R., Webb, Milad, Stewart, Travis J., Phillips, Travis, Carter, Michael, Cremo, Christine R., Baker, Josh E.

“…•Sucrose slows actin sliding velocities by inhibiting the rate of actin–myosin strong binding.•Sucrose specifically inhibits actin–myosin strong bond formation…”
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Myosin light chain kinase steady-state kinetics: comparison of smooth muscle myosin II and nonmuscle myosin IIB as substrates by Alcala, Diego B., Haldeman, Brian D., Brizendine, Richard K., Krenc, Agata K., Baker, Josh E., Rock, Ronald S., Cremo, Christine R.

“…Myosin light chain kinase (MLCK) phosphorylates S19 of the myosin regulatory light chain (RLC), which is required to activate myosin's ATPase activity and…”
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The Myosin Duty Ratio Tunes the Calcium Sensitivity and Cooperative Activation of the Thin Filament by Webb, Milad, Jackson, Del R, Stewart, Travis J, Dugan, Samuel P, Carter, Michael S, Cremo, Christine R, Baker, Josh E

“…In striated muscle, calcium binding to the thin filament (TF) regulatory complex activates actin–myosin ATPase activity, and actin–myosin kinetics in turn…”
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