Search Results - "Craig, Jonathan M"

Revealing dynamics of helicase translocation on single-stranded DNA using high-resolution nanopore tweezers by Craig, Jonathan M., Laszlo, Andrew H., Brinkerhoff, Henry, Derrington, Ian M., Noakes, Matthew T., Nova, Ian C., Tickman, Benjamin I., Doering, Kenji, de Leeuw, Noah F., Gundlach, Jens H.

“…Enzymes that operate on DNA or RNA perform the core functions of replication and expression in all of biology. To gain high-resolution access to the detailed…”
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Nanopores map the acid-base properties of a single site in a single DNA molecule by Smith, Drew C, Thomas, Christopher A, Craig, Jonathan M, Brinkerhoff, Henry, Abell, Sarah J, Franzi, Michaela C, Carrasco, Jessica D, Hoshika, Shuichi, Benner, Steven A, Gundlach, Jens H, Laszlo, Andrew H

“…Abstract Nanopores are increasingly powerful tools for single molecule sensing, in particular, for sequencing DNA, RNA and peptides. This success has spurred…”
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Observing inhibition of the SARS-CoV-2 helicase at single-nucleotide resolution by Marx, Sinduja K, Mickolajczyk, Keith J, Craig, Jonathan M, Thomas, Christopher A, Pfeffer, Akira M, Abell, Sarah J, Carrasco, Jessica D, Franzi, Michaela C, Huang, Jesse R, Kim, Hwanhee C, Brinkerhoff, Henry, Kapoor, Tarun M, Gundlach, Jens H, Laszlo, Andrew H

“…The genome of SARS-CoV-2 encodes for a helicase (nsp13) that is essential for viral replication and highly conserved across related viruses, making it an…”
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Enzymatic synthesis and nanopore sequencing of 12-letter supernumerary DNA by Kawabe, Hinako, Thomas, Christopher A., Hoshika, Shuichi, Kim, Myong-Jung, Kim, Myong-Sang, Miessner, Logan, Kaplan, Nicholas, Craig, Jonathan M., Gundlach, Jens H., Laszlo, Andrew H., Benner, Steven A., Marchand, Jorge A.

“…The 4-letter DNA alphabet (A, T, G, C) as found in Nature is an elegant, yet non-exhaustive solution to the problem of storage, transfer, and evolution of…”
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Investigating asymmetric salt profiles for nanopore DNA sequencing with biological porin MspA by Nova, Ian C, Derrington, Ian M, Craig, Jonathan M, Noakes, Matthew T, Tickman, Benjamin I, Doering, Kenji, Higinbotham, Hugh, Laszlo, Andrew H, Gundlach, Jens H

“…Nanopore DNA sequencing is a promising single-molecule analysis technology. This technique relies on a DNA motor enzyme to control movement of DNA precisely…”
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Direct Detection of Unnatural DNA Nucleotides dNaM and d5SICS using the MspA Nanopore by Craig, Jonathan M, Laszlo, Andrew H, Derrington, Ian M, Ross, Brian C, Brinkerhoff, Henry, Nova, Ian C, Doering, Kenji, Tickman, Benjamin I, Svet, Mark T, Gundlach, Jens H

“…Malyshev et al. showed that the four-letter genetic code within a living organism could be expanded to include the unnatural DNA bases dNaM and d5SICS…”
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Decoding long nanopore sequencing reads of natural DNA by Laszlo, Andrew H, Derrington, Ian M, Ross, Brian C, Brinkerhoff, Henry, Adey, Andrew, Nova, Ian C, Craig, Jonathan M, Langford, Kyle W, Samson, Jenny Mae, Daza, Riza, Doering, Kenji, Shendure, Jay, Gundlach, Jens H

“…Laszlo et al . demonstrate sequence alignment, and proof-of-concept organism identification, genome assembly and polymorphism detection from nanopore analysis…”
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Subangstrom single-molecule measurements of motor proteins using a nanopore by Derrington, Ian M, Craig, Jonathan M, Stava, Eric, Laszlo, Andrew H, Ross, Brian C, Brinkerhoff, Henry, Nova, Ian C, Doering, Kenji, Tickman, Benjamin I, Ronaghi, Mostafa, Mandell, Jeffrey G, Gunderson, Kevin L, Gundlach, Jens H

“…The movement of single motor proteins on DNA is measured at subangstrom resolution using a nanopore. Techniques for measuring the motion of single motor…”
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UPF1 mutants with intact ATPase but deficient helicase activities promote efficient nonsense-mediated mRNA decay by Chapman, Joseph H, Craig, Jonathan M, Wang, Clara D, Gundlach, Jens H, Neuman, Keir C, Hogg, J Robert

“…The conserved RNA helicase UPF1 coordinates nonsense-mediated mRNA decay (NMD) by engaging with mRNAs, RNA decay machinery and the terminating ribosome. UPF1…”
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Assessing Readability of an 8‑Letter Expanded Deoxyribonucleic Acid Alphabet with Nanopores by Thomas, Christopher A., Craig, Jonathan M., Hoshika, Shuichi, Brinkerhoff, Henry, Huang, Jesse R., Abell, Sarah J., Kim, Hwanhee C., Franzi, Michaela C., Carrasco, Jessica D., Kim, Hyo-Joong, Smith, Drew C., Gundlach, Jens H., Benner, Steven A., Laszlo, Andrew H.

“…Chemists have now synthesized new kinds of DNA that add nucleotides to the four standard nucleotides (guanine, adenine, cytosine, and thymine) found in…”
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Nanopore tweezers show fractional-nucleotide translocation in sequence-dependent pausing by RNA polymerase by Nova, Ian C, Craig, Jonathan M, Mazumder, Abhishek, Laszlo, Andrew H, Derrington, Ian M, Noakes, Matthew T, Brinkerhoff, Henry, Yang, Shuya, Vahedian-Movahed, Hanif, Li, Lingting, Zhang, Yu, Bowman, Jasmine L, Huang, Jesse R, Mount, Jonathan W, Ebright, Richard H, Gundlach, Jens H

“…RNA polymerases (RNAPs) carry out the first step in the central dogma of molecular biology by transcribing DNA into RNA. Despite their importance, much about…”
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Nanopore tweezers measurements of RecQ conformational changes reveal the energy landscape of helicase motion by Craig, Jonathan M, Mills, Maria, Kim, Hwanhee C, Huang, Jesse R, Abell, Sarah J, Mount, Jonathan W, Gundlach, Jens H, Neuman, Keir C, Laszlo, Andrew H

“…Helicases are essential for nearly all nucleic acid processes across the tree of life, yet detailed understanding of how they couple ATP hydrolysis to…”
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Determining the effects of DNA sequence on Hel308 helicase translocation along single-stranded DNA using nanopore tweezers by Craig, Jonathan M, Laszlo, Andrew H, Nova, Ian C, Brinkerhoff, Henry, Noakes, Matthew T, Baker, Katherine S, Bowman, Jasmine L, Higinbotham, Hugh R, Mount, Jonathan W, Gundlach, Jens H

“…Abstract Motor enzymes that process nucleic-acid substrates play vital roles in all aspects of genome replication, expression, and repair. The DNA and RNA…”
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Nanopore Sequencing of an Expanded Genetic Alphabet Reveals High-Fidelity Replication of a Predominantly Hydrophobic Unnatural Base Pair by Ledbetter, Michael P, Craig, Jonathan M, Karadeema, Rebekah J, Noakes, Matthew T, Kim, Hwanhee C, Abell, Sarah J, Huang, Jesse R, Anderson, Brooke A, Krishnamurthy, Ramanarayanan, Gundlach, Jens H, Romesberg, Floyd E

“…Unnatural base pairs (UBPs) have been developed and used for a variety of in vitro applications as well as for the engineering of semisynthetic organisms…”
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Sequence-dependent mechanochemical coupling of helicase translocation and unwinding at single-nucleotide resolution by Laszlo, Andrew H, Craig, Jonathan M, Gavrilov, Momčilo, Tippana, Ramreddy, Nova, Ian C, Huang, Jesse R, Kim, Hwanhee C, Abell, Sarah J, deCampos-Stairiker, Mallory, Mount, Jonathan W, Bowman, Jasmine L, Baker, Katherine S, Higinbotham, Hugh, Bobrovnikov, Dmitriy, Ha, Taekjip, Gundlach, Jens H

“…We used single-molecule picometer-resolution nanopore tweezers (SPRNT) to resolve the millisecond single-nucleotide steps of superfamily 1 helicase PcrA as it…”
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Mechanism of Sequence Dependent Translocation of a Superfamily 2 Helicase on ssDNA by Craig, Jonathan M.

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Taking a Closer Look at RecQ Helicase with Nanopore Tweezers by Craig, Jonathan M., Mills, K. Maria, Laszlo, Andrew H., Neuman, Keir C., Gundlach, Jens H.

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C-Glycoside DNA bases from an artificially expanded genetic information system reduce processivity in a SF2 helicase as revealed by nanopore tweezers by Thomas, Christopher A., Craig, Jonathan M., Hoshika, Shuichi, Laszlo, Andrew H., Huang, Jesse R., Abell, Sarah J., Kim, Hwanhee C., Carrasco, Jessica, Franzi, Michaela C., Benner, Steven A., Gundlach, Jens H.

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SV40 Large T Antigen Helicase Unwinds Double-Stranded DNA in Single-Nucleotide Steps as Revealed by Nanopore Tweezers by Thomas, Christopher A., Craig, Jonathan M., Laszlo, Andrew H., Nova, Ian C., Xie, Sherry, Huang, Jesse R., Kim, Hwanhee C., Abell, Sarah J., Yardimci, Hasan, Gundlach, Jens H.

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Uncovering the Molecular Basis for Sequence-Dependent Translocation in a Superfamily 2 Helicase using High-Resolution Nanopore Tweezers by Craig, Jonathan M., Marx, Sinduja K., Laszlo, Andrew H., Nova, Ian C., Kim, Hwanhee C., Huang, Jesse R., Abell, Sarah J., Gundlach, Jens H.

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