Search Results - "Crack, Jason"

Synergy of native mass spectrometry and other biophysical techniques in studies of iron‑sulfur cluster proteins and their assembly by Crack, Jason C., Le Brun, Nick E.

“…The application of mass spectrometric methodologies has revolutionised biological chemistry, from identification through to structural and conformational…”
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Site-specific encoding of photoactivity and photoreactivity into antibody fragments by Bridge, Thomas, Wegmann, Udo, Crack, Jason C., Orman, Kate, Shaikh, Saher A., Farndon, William, Martins, Carlo, Saalbach, Gerhard, Sachdeva, Amit

“…Design of biomolecules that perform two or more distinct functions in response to light remains challenging. Here, we have introduced concurrent photoactivity…”
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Binding of a single nitric oxide molecule is sufficient to disrupt DNA binding of the nitrosative stress regulator NsrR by Crack, Jason C, Le Brun, Nick E

“…The regulatory protein NsrR, a member of the Rrf2 protein superfamily, plays a major role in the cellular response to nitrosative stress in many benign and…”
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Mass spectrometric identification of intermediates in the O 2 -driven [4Fe-4S] to [2Fe-2S] cluster conversion in FNR by Crack, Jason C, Thomson, Andrew J, Le Brun, Nick E

“…The iron-sulfur cluster containing protein Fumarate and Nitrate Reduction (FNR) is the master regulator for the switch between anaerobic and aerobic…”
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Iron–Sulfur Clusters as Biological Sensors: The Chemistry of Reactions with Molecular Oxygen and Nitric Oxide by Crack, Jason C, Green, Jeffrey, Thomson, Andrew J, Brun, Nick E. Le

“…Conspectus Iron–sulfur cluster proteins exhibit a range of physicochemical properties that underpin their functional diversity in biology, which includes roles…”
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Mass spectrometric identification of intermediates in the O₂-driven [4Fe-4S] to [2Fe-2S] cluster conversion in FNR by Crack, Jason C., Thomson, Andrew J., Le Brun, Nick E.

“…The iron-sulfur cluster containing protein Fumarate and Nitrate Reduction (FNR) is the master regulator for the switch between anaerobic and aerobic…”
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Mass Spectrometric Identification of [4Fe–4S](NO)x Intermediates of Nitric Oxide Sensing by Regulatory Iron–Sulfur Cluster Proteins by Crack, Jason C., Le Brun, Nick E.

“…Nitric oxide (NO) can function as both a cytotoxin and a signalling molecule. In both cases, reaction with iron–sulfur (Fe–S) cluster proteins plays an…”
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Native mass spectrometric studies of IscSU reveal a concerted, sulfur-initiated mechanism of iron-sulfur cluster assembly by Bennett, Sophie P, Crack, Jason C, Puglisi, Rita, Pastore, Annalisa, Le Brun, Nick E

“…Iron-sulfur (Fe-S) clusters are cofactors essential for life. Though the proteins that function in the assembly of Fe-S clusters are well known, details of the…”
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Polyurethane infused with heparin capped silver nanoparticles dressing for wound healing application: Synthesis, characterization and antimicrobial studies by Ahire, Jayshree H., Wang, Qi, Rowley, Gary, Chambrier, Isabelle, Crack, Jason C., Bao, Yongping, Chao, Yimin

“…Burn and diabetic wounds present significant challenges due to their complex nature, delayed healing, pain, and high susceptibility to bacterial infections. In…”
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Mycobacterium tuberculosis WhiB1 is an essential DNA-binding protein with a nitric oxide-sensitive iron-sulfur cluster by Smith, Laura J, Stapleton, Melanie R, Fullstone, Gavin J M, Crack, Jason C, Thomson, Andrew J, Le Brun, Nick E, Hunt, Debbie M, Harvey, Evelyn, Adinolfi, Salvatore, Buxton, Roger S, Green, Jeffrey

“…Mycobacterium tuberculosis is a major pathogen that has the ability to establish, and emerge from, a persistent state. Wbl family proteins are associated with…”
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Stabilisation of the RirA [4Fe-4S] cluster results in loss of iron-sensing function by Gray, Elizabeth, Stewart, Melissa Y. Y, Hanwell, Libby, Crack, Jason C, Devine, Rebecca, Stevenson, Clare E. M, Volbeda, Anne, Johnston, Andrew W. B, Fontecilla-Camps, Juan C, Hutchings, Matthew I, Todd, Jonathan D, Le Brun, Nick E

“…RirA is a global iron regulator in diverse Alphaproteobacteria that belongs to the Rrf2 superfamily of transcriptional regulators, which can contain an…”
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Native Mass Spectrometry of Iron-Sulfur Proteins by Crack, Jason C, Le Brun, Nick E

“…Iron-sulfur clusters constitute a large and widely distributed group of protein cofactors that play key roles in a wide range of metabolic processes. The…”
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Crystal structures of the NO sensor NsrR reveal how its iron-sulfur cluster modulates DNA binding by Volbeda, Anne, Dodd, Erin L., Darnault, Claudine, Crack, Jason C., Renoux, Oriane, Hutchings, Matthew I., Le Brun, Nick E., Fontecilla-Camps, Juan C.

“…NsrR from Streptomyces coelicolor ( Sc) regulates the expression of three genes through the progressive degradation of its [4Fe–4S] cluster on nitric oxide…”
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Liquid-chromatography mass spectrometry describes post-translational modification of Shewanella outer membrane proteins by van Wonderen, Jessica H., Crack, Jason C., Edwards, Marcus J., Clarke, Thomas A., Saalbach, Gerhard, Martins, Carlo, Butt, Julea N.

“…Electrogenic bacteria deliver excess respiratory electrons to externally located metal oxide particles and electrodes. The biochemical basis for this process…”
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Crystal Structure of the Transcription Regulator RsrR Reveals a [2Fe–2S] Cluster Coordinated by Cys, Glu, and His Residues by Volbeda, Anne, Martinez, Ma Teresa Pellicer, Crack, Jason C, Amara, Patricia, Gigarel, Océane, Munnoch, John T, Hutchings, Matthew I, Darnault, Claudine, Le Brun, Nick E, Fontecilla-Camps, Juan C

“…The recently discovered Rrf2 family transcriptional regulator RsrR coordinates a [2Fe–2S] cluster. Remarkably, binding of the protein to RsrR-regulated…”
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The transcriptional repressor protein NsrR senses nitric oxide directly via a [2Fe-2S] cluster by Tucker, Nicholas P, Hicks, Matthew G, Clarke, Thomas A, Crack, Jason C, Chandra, Govind, Le Brun, Nick E, Dixon, Ray, Hutchings, Matthew I

“…The regulatory protein NsrR, a member of the Rrf2 family of transcription repressors, is specifically dedicated to sensing nitric oxide (NO) in a variety of…”
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Reversible cycling between cysteine persulfide-ligated [2Fe-2S] and cysteine-ligated [4Fe-4S] clusters in the FNR regulatory protein by Zhang, Bo, Crack, Jason C, Subramanian, Sowmya, Green, Jeffrey, Thomson, Andrew J, Le Brun, Nick E, Johnson, Michael K

“…Fumarate and nitrate reduction (FNR) regulatory proteins are O ₂-sensing bacterial transcription factors that control the switch between aerobic and anaerobic…”
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The Di-Iron Protein YtfE Is a Nitric Oxide-Generating Nitrite Reductase Involved in the Management of Nitrosative Stress by Crack, Jason C, Balasiny, Basema K, Bennett, Sophie P, Rolfe, Matthew D, Froes, Afonso, MacMillan, Fraser, Green, Jeffrey, Cole, Jeffrey A, Le Brun, Nick E

“…Previously characterized nitrite reductases fall into three classes: siroheme-containing enzymes (NirBD), cytochrome c hemoproteins (NrfA and NirS), and…”
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Structure of a Wbl protein and implications for NO sensing by M. tuberculosis by Kudhair, Bassam K., Hounslow, Andrea M., Rolfe, Matthew D., Crack, Jason C., Hunt, Debbie M., Buxton, Roger S., Smith, Laura J., Le Brun, Nick E., Williamson, Michael P., Green, Jeffrey

“…Mycobacterium tuberculosis causes pulmonary tuberculosis (TB) and claims ~1.8 million human lives per annum. Host nitric oxide (NO) is important in controlling…”
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Reaction of Thiosulfate Dehydrogenase with a Substrate Mimic Induces Dissociation of the Cysteine Heme Ligand Giving Insights into the Mechanism of Oxidative Catalysis by Jenner, Leon P., Crack, Jason C., Kurth, Julia M., Soldánová, Zuzana, Brandt, Linda, Sokol, Katarzyna P., Reisner, Erwin, Bradley, Justin M., Dahl, Christiane, Cheesman, Myles R., Butt, Julea N.

“…Thiosulfate dehydrogenases are bacterial cytochromes that contribute to the oxidation of inorganic sulfur. The active sites of these enzymes contain low-spin…”
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