Whole transcriptome analysis of the fasting and fed Burmese python heart: insights into extreme physiological cardiac adaptation

The infrequently feeding Burmese python (Python molurus) experiences significant and rapid postprandial cardiac hypertrophy followed by regression as digestion is completed. To begin to explore the molecular mechanisms of this response, we have sequenced and assembled the fasted and postfed Burmese...

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Bibliographic Details
Published in:	Physiological genomics Vol. 43; no. 2; pp. 69 - 76
Main Authors:	Wall, Christopher E, Cozza, Steven, Riquelme, Cecilia A, McCombie, W Richard, Heimiller, Joseph K, Marr, Thomas G, Leinwand, Leslie A
Format:	Journal Article
Language:	English
Published:	United States American Physiological Society 01-01-2011
Subjects:	Adaptation, Physiological - genetics Adaptation, Physiological - physiology Animals Base Pairing - genetics Boidae - genetics Boidae - physiology Call for Papers: Technology Development for Physiological Systems Fasting - physiology Feeding Behavior - physiology Gallus gallus Gene Expression Profiling - methods Gene Expression Regulation Heart - physiology Humans Hypertrophy Molecular Sequence Annotation Molecular Sequence Data Myanmar Myocardium - metabolism Myocardium - pathology Postprandial Period - genetics Postprandial Period - physiology Python molurus RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Analysis, DNA Sequence Homology, Nucleic Acid Signal Transduction - genetics Time Factors Myanmar
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Summary:	The infrequently feeding Burmese python (Python molurus) experiences significant and rapid postprandial cardiac hypertrophy followed by regression as digestion is completed. To begin to explore the molecular mechanisms of this response, we have sequenced and assembled the fasted and postfed Burmese python heart transcriptomes with Illumina technology using the chicken (Gallus gallus) genome as a reference. In addition, we have used RNA-seq analysis to identify differences in the expression of biological processes and signaling pathways between fasted, 1 day postfed (DPF), and 3 DPF hearts. Out of a combined transcriptome of ∼2,800 mRNAs, 464 genes were differentially expressed. Genes showing differential expression at 1 DPF compared with fasted were enriched for biological processes involved in metabolism and energetics, while genes showing differential expression at 3 DPF compared with fasted were enriched for processes involved in biogenesis, structural remodeling, and organization. Moreover, we present evidence for the activation of physiological and not pathological signaling pathways in this rapid, novel model of cardiac growth in pythons. Together, our data provide the first comprehensive gene expression profile for a reptile heart.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 C. E. Wall and S. Cozza contributed equally to this work.
ISSN:	1094-8341 1531-2267
DOI:	10.1152/physiolgenomics.00162.2010