A cell line infectible by prion strains from different species

It has been shown previously that ovine prion protein (PrPC) renders rabbit epithelial RK13 cells permissive to the multiplication of ovine prions, thus providing evidence that species barriers can be crossed in cultured cells through the expression of a relevant PrPC. The present study significantl...

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Bibliographic Details
Published in:	Journal of general virology Vol. 89; pp. 341 - 347
Main Authors:	Courageot, M.P., Daude, Nathalie, Nonno, R., Paquet, Sophie, Di Bari, M.A., Le Dur, Annick, Chapuis, Jerome, Hill, A.F., Agrimi, U., Laude, Hubert H., Vilette, Didier
Format:	Journal Article
Language:	English
Published:	Microbiology Society 2008
Subjects:	Life Sciences Microbiology and Parasitology Virology
Online Access:	Get full text
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Summary:	It has been shown previously that ovine prion protein (PrPC) renders rabbit epithelial RK13 cells permissive to the multiplication of ovine prions, thus providing evidence that species barriers can be crossed in cultured cells through the expression of a relevant PrPC. The present study significantly extended this observation by showing that mouse and bank vole prions can be propagated in RK13 cells that express the corresponding PrPC. Importantly, the respective molecular patterns of abnormal PrP (PrPres) and, where examined, the neuropathological features of the infecting strains appeared to be maintained during the propagation in cell culture. These findings indicate that RK13 cells can be genetically engineered to replicate prion strains faithfully from different species. Such an approach may facilitate investigations of the molecular basis of strain identity and prion diversity.
ISSN:	0022-1317 1465-2099
DOI:	10.1099/vir.0.83344-0