Search Results - "Coull, James"

Peptide nucleic acid–dependent artifact can lead to false-positive triplex gene editing signals by Ho, Pui Yan, Zhang, Zhen, Hayes, Mark E., Curd, Andrew, Dib, Carla, Rayburn, Maire, Tam, Sze Nok, Srivastava, Tumul, Hriniak, Brandon, Li, Xiao-Jun, Leonard, Scott, Wang, Lan, Tarighat, Somayeh, Sim, Derek S., Fiandaca, Mark, Coull, James M., Ebens, Allen, Fordyce, Marshall, Czechowicz, Agnieszka

“…Triplex gene editing relies on binding a stable peptide nucleic acid (PNA) sequence to a chromosomal target, which alters the helical structure of DNA to…”
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Diamine Catalyzed Hemicyanine Dye Formation from Nonfluorescent Precursors through DNA Programmed Chemistry by Huang, Yumei, Coull, James M

“…A hemicyanine fluorescent dye was generated by a diamine-catalyzed, DNA-templated, aldol-type condensation from nonfluorescent precursors. Studies of reaction…”
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Multicenter Evaluation of the Staphylococcus QuickFISH Method for Simultaneous Identification of Staphylococcus aureus and Coagulase-Negative Staphylococci Directly from Blood Culture Bottles in Less than 30 Minutes by DECK, Melissa K, ANDERSON, Erica S, HAZEN, Kevin, KLIMAS, Lisa L, LINDAO, Daniel, MELTZER, Michelle C, MORGAN, Margie, SHEPARD, Janeen, STEVENS, Sharon, FANN WU, FIANDACA, Mark J, BUCKNER, Rebecca J, COLASANTE, Georgia, COULL, James M, CRYSTAL, Benjamin, LATTA, Phyllis Della, FUCHS, Martin, FULLER, Deanna, HARRIS, Will

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Hybridization of DNA and PNA Molecular Beacons to Single-Stranded and Double-Stranded DNA Targets by Kuhn, Heiko, Demidov, Vadim V., Coull, James M., Fiandaca, Mark J., Gildea, Brian D., Frank-Kamenetskii, Maxim D.

“…Molecular beacons are sensitive fluorescent probes hybridizing selectively to designated DNA and RNA targets. They have recently become practical tools for…”
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Rapid detection of Enterococcus spp. direct from blood culture bottles using Enterococcus QuickFISH Method: a multicenter investigation by Deck, Melissa K., Anderson, Erica S., Buckner, Rebecca J., Colasante, Georgia, Davis, Thomas E., Coull, James M., Crystal, Benjamin, Latta, Phyllis Della, Fuchs, Martin, Fuller, Deanna, Harris, Will, Hazen, Kevin, Klimas, Lisa L., Lindao, Daniel, Meltzer, Michelle C., Morgan, Margie, Shepard, Janeen, Stevens, Sharon, Wu, Fann, Fiandaca, Mark J.

“…The performance of a diagnostic method for detection and identification of Enterococcus spp. directly from positive blood culture was evaluated in a clinical…”
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Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes by OLIVEIRA, Kenneth, PROCOP, Gary W, WILSON, Deborah, COULL, James, STENDER, Henrik

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Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes for Rapid Identification of Candida albicans Directly from Blood Culture Bottles by RIGBY, Susan, PROCOP, Gary W, STENDER, Henrik, HAASE, Gerhard, WILSON, Deborah, HALL, Geraldine, KURTZMAN, Cletus, OLIVEIRA, Kenneth, VON OY, Sabina, HYLDIG-NIELSEN, Jens J, COULL, James

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Identification of indicator microorganisms using a standardized PNA FISH method by Perry-O'Keefe, Heather, Rigby, Susan, Oliveira, Kenneth, Sørensen, Ditte, Stender, Henrik, Coull, James, Hyldig-Nielsen, J.J.

“…A standardized fluorescent in situ hybridization (FISH) method using Peptide Nucleic Acid (PNA) probes for analysis of gram-negative and gram-positive…”
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Identification of Dekkera bruxellensis(Brettanomyces) from Wine by Fluorescence In Situ Hybridization Using Peptide Nucleic Acid Probes by STENDER, Henrik, KURTZMAN, Cletus, HYLDIG-NIELSEN, Jens J, SØRENSEN, Ditte, BROOMER, Adam, OLIVEIRA, Kenneth, PERRY-O'KEEFE, Heather, SAGE, Andrew, YOUNG, Barbara, COULL, James

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Peptide Nucleic Acid Pre-Gel Hybridization: An Alternative to Southern Hybridization by Perry-O'Keefe, Heather, Yao, Xian-Wei, Coull, James M., Fuchs, Martin, Egholm, Michael

“…We have found that it is possible to use labeled peptide nucleic acid (PNA)-oligomers as probes in pre-gel hybridization experiments, as an alternative for…”
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PNA fluorescent in situ hybridization for rapid microbiology and cytogenetic analysis by Williams, Brett, Stender, Henrik, Coull, James M

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Solid-phase synthesis of peptide nucleic acids by Christensen, L, Fitzpatrick, R, Gildea, B, Petersen, K H, Hansen, H F, Koch, T, Egholm, M, Buchardt, O, Nielsen, P E, Coull, J, Berg, R H

“…Peptide nucleic acids (PNA) were synthesized by a modified Merrifield method using several improvements. Activation by…”
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Herring. A History of the Silver Darlings (review) by Coull, James R

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Rapid detection, identification, and enumeration of Escherichia coli by fluorescence in situ hybridization using an array scanner by Stender, Henrik, Oliveira, Kenneth, Rigby, Susan, Bargoot, Frederick, Coull, James

“…A new fluorescence in situ hybridization (FISH) method using peptide nucleic acid (PNA) probes and an array scanner for rapid detection, identification, and…”
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DNA-programmed chemistry in rapid homogeneous assays for DNA and protein targets by Haff, Lawrence A, Wilson, Christopher G M, Huang, Yumei, Benton, Benjamin K, Bond, Julian F, Stern, Andrew M, Begley, Richard F, Coull, James M

“…Under experimental conditions of pH 8, 25 °C, 10 mmol/L magnesium chloride, and 350 mL formamide, the complementary reporter sequences of the oligonucleotides…”
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Multicolor fluorescence in situ hybridization with peptide nucleic acid probes for enumeration of specific chromosomes in human cells by Taneja, Krishan L., Chavez, Elizabeth A., Coull, James, Lansdorp, Peter M.

“…In previous studies, we showed that peptide nucleic acid (PNA) probes have significant advantages over conventional synthetic RNA or DNA probes in FISH…”
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The Development of Herring Fishing in the Outer Hebrides by Coull, James R.

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Biochemical Evidence That a D-Loop Is Part of a Four-Stranded PNA−DNA Bundle. Nickel-Mediated Cleavage of Duplex DNA by a Gly-Gly-His Bis-PNA by Footer, Matthew, Egholm, Michael, Kron, Steve, Coull, James M, Matsudaira, Paul

“…A peptide nucleic acid (PNA) with improved strand-displacement capability and a site-specific DNA cleavage function is a novel reagent for probing the…”
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Development of a selection assay for small guide RNAs that drive efficient site-directed RNA editing by Diaz Quiroz, Juan Felipe, Ojha, Namrata, Shayhidin, Elnur E, De Silva, Dasuni, Dabney, Jesse, Lancaster, Amy, Coull, James, Milstein, Stuart, Fraley, Andrew W, Brown, Christopher R, Rosenthal, Joshua J C

“…Abstract A major challenge confronting the clinical application of site-directed RNA editing (SDRE) is the design of small guide RNAs (gRNAs) that can drive…”
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Identification of Optimized Peptide Nucleic Acid (PNA) Designs for Nuclease-Free Gene Editing in Sickle Cell Disease Hematopoietic Stem and Progenitor Cells (HSPCs) by Ebens, Allen, Curd, Andrew, Sim, Derek, Quijano, Elias, Tam, Florence, Coull, James, Huang, Lily, Hayes, Mark, Fordyce, Marshall, Pearson, Robert, Whoriskey, Susan, Srivastava, Tumul, Li, Xiaojun, Zhang, Zhen

“…Homozygous E6V alleles in the beta globin gene cause disease in the majority of sickle cell patients and the use of gene editing technologies to correct this…”
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