Search Results - "Costa, Magdaline"

Targeting a GFP reporter gene to the MIXL1 locus of human embryonic stem cells identifies human primitive streak–like cells and enables isolation of primitive hematopoietic precursors by Davis, Richard P., Ng, Elizabeth S., Costa, Magdaline, Mossman, Anna K., Sourris, Koula, Elefanty, Andrew G., Stanley, Edouard G.

“…Differentiating human embryonic stem cells (HESCs) represent an experimental platform for establishing the relationships between the earliest lineages that…”
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APELIN promotes hematopoiesis from human embryonic stem cells by Yu, Qing C., Hirst, Claire E., Costa, Magdaline, Ng, Elizabeth S., Schiesser, Jacqueline V., Gertow, Karin, Stanley, Edouard G., Elefanty, Andrew G.

“…Transcriptional profiling of differentiating human embryonic stem cells (hESCs) revealed that MIXL1-positive mesodermal precursors were enriched for…”
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A method for genetic modification of human embryonic stem cells using electroporation by Stanley, Edouard G, Costa, Magdaline, Dottori, Mirella, Sourris, Koula, Jamshidi, Pegah, Hatzistavrou, Tanya, Davis, Richard, Azzola, Lisa, Jackson, Steven, Lim, Sue Mei, Pera, Martin, Elefanty, Andrew G

“…The ability to genetically modify human embryonic stem cells (HESCs) will be critical for their widespread use as a tool for understanding fundamental aspects…”
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SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development by Skelton, Rhys J.P., Costa, Magdaline, Anderson, David J., Bruveris, Freya, Finnin, Ben W., Koutsis, Katerina, Arasaratnam, Deevina, White, Anthony J., Rafii, Arash, Ng, Elizabeth S., Elefanty, Andrew G., Stanley, Edouard G., Pouton, Colin W., Haynes, John M., Ardehali, Reza, Davis, Richard P., Mummery, Christine L., Elliott, David A.

“…The study of human cardiogenesis would benefit from a detailed cell lineage fate map akin to that established for the haematopoietic lineages. Here we sought…”
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GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives by Kao, Tim, Labonne, Tanya, Niclis, Jonathan C., Chaurasia, Ritu, Lokmic, Zerina, Qian, Elizabeth, Bruveris, Freya F., Howden, Sara E., Motazedian, Ali, Schiesser, Jacqueline V., Costa, Magdaline, Sourris, Koula, Ng, Elizabeth, Anderson, David, Giudice, Antonietta, Farlie, Peter, Cheung, Michael, Lamande, Shireen R., Penington, Anthony J., Parish, Clare L., Thomson, Lachlan H., Rafii, Arash, Elliott, David A., Elefanty, Andrew G., Stanley, Edouard G.

“…The ability to reliably express fluorescent reporters or other genes of interest is important for using human pluripotent stem cells (hPSCs) as a platform for…”
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A protocol for removal of antibiotic resistance cassettes from human embryonic stem cells genetically modified by homologous recombination or transgenesis by Stanley, Edouard G, Davis, Richard P, Costa, Magdaline, Grandela, Catarina, Holland, Andrew M, Hatzistavrou, Tanya, Micallef, Suzanne J, Li, Xueling, Goulburn, Adam L, Azzola, Lisa, Elefanty, Andrew G

“…The first step in the generation of genetically tagged human embryonic stem cell (HESC) reporter lines is the isolation of cells that contain a stably…”
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Differentiation of human embryonic stem cells to HOXA+ hemogenic vasculature that resembles the aorta-gonad-mesonephros by Ng, Elizabeth S, Azzola, Lisa, Bruveris, Freya F, Calvanese, Vincenzo, Phipson, Belinda, Vlahos, Katerina, Hirst, Claire, Jokubaitis, Vanta J, Yu, Qing C, Maksimovic, Jovana, Liebscher, Simone, Januar, Vania, Zhang, Zhen, Williams, Brenda, Conscience, Aude, Durnall, Jennifer, Jackson, Steven, Costa, Magdaline, Elliott, David, Haylock, David N, Nilsson, Susan K, Saffery, Richard, Schenke-Layland, Katja, Oshlack, Alicia, Mikkola, Hanna K A, Stanley, Edouard G, Elefanty, Andrew G

“…Human embryonic stem cells are differentiated to cells similar to the embryonic aorta-gonad-mesonephros, which gives rise to hematopoietic stem cells. The…”
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Human endothelial cell-derived nuclear proteins that recognise polymorphic DNA elements in the von Willebrand factor gene promoter include YY1 by Costa, M, Grant, P J, Rice, G I, Futers, T S, Medcalf, R L

“…Four common base-change polymorphisms have been found in the von Willebrand factor gene promoter: (-1793 C/G, -1234 T/C, -1185 G/A and -1051 A/G). All four…”
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ZEB2 drives immature T-cell lymphoblastic leukaemia development via enhanced tumour-initiating potential and IL-7 receptor signalling by Goossens, Steven, Radaelli, Enrico, Blanchet, Odile, Durinck, Kaat, Van der Meulen, Joni, Peirs, Sofie, Taghon, Tom, Tremblay, Cedric S., Costa, Magdaline, Ghahremani, Morvarid Farhang, De Medts, Jelle, Bartunkova, Sonia, Haigh, Katharina, Schwab, Claire, Farla, Natalie, Pieters, Tim, Matthijssens, Filip, Van Roy, Nadine, Best, J. Adam, Deswarte, Kim, Bogaert, Pieter, Carmichael, Catherine, Rickard, Adam, Suryani, Santi, Bracken, Lauryn S., Alserihi, Raed, Canté-Barrett, Kirsten, Haenebalcke, Lieven, Clappier, Emmanuelle, Rondou, Pieter, Slowicka, Karolina, Huylebroeck, Danny, Goldrath, Ananda W., Janzen, Viktor, McCormack, Matthew P., Lock, Richard B., Curtis, David J., Harrison, Christine, Berx, Geert, Speleman, Frank, Meijerink, Jules P. P., Soulier, Jean, Van Vlierberghe, Pieter, Haigh, Jody J.

“…Early T-cell precursor leukaemia (ETP-ALL) is a high-risk subtype of human leukaemia that is poorly understood at the molecular level. Here we report…”
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Oncogenic ZEB2 activation drives sensitivity toward KDM1A inhibition in T-cell acute lymphoblastic leukemia by Goossens, Steven, Peirs, Sofie, Van Loocke, Wouter, Wang, Jueqiong, Takawy, Mina, Matthijssens, Filip, Sonderegger, Stefan E., Haigh, Katharina, Nguyen, Thao, Vandamme, Niels, Costa, Magdaline, Carmichael, Catherine, Van Nieuwerburgh, Filip, Deforce, Dieter, Kleifeld, Oded, Curtis, David J., Berx, Geert, Van Vlierberghe, Pieter, Haigh, Jody J.

“…Elevated expression of the Zinc finger E-box binding homeobox transcription factor-2 (ZEB2) is correlated with poor prognosis and patient outcome in a variety…”
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Abstract B04: A role for EphA3 in angiogenesis, in vivo and in vitro exploration by Farnsworth, Rae H., Vail, Mary E., Costa, Magdaline, Hii, Linda, Dickins, Ross A., Elefanty, Andrew G., Stanley, Edouard G., Lackmann, Martin

“…Abstract The Eph family of receptor tyrosine kinases, and their ephrin ligands, are key regulators of cell migration and tissue patterning which are crucial…”
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Differentiation of human embryonic stem cells to HOXA super(+) hemogenic vasculature that resembles the aorta-gonad-mesonephros by Ng, Elizabeth S, Azzola, Lisa, Bruveris, Freya F, Calvanese, Vincenzo, Phipson, Belinda, Vlahos, Katerina, Hirst, Claire, Jokubaitis, Vanta J, Yu, Qing C, Maksimovic, Jovana, Liebscher, Simone, Januar, Vania, Zhang, Zhen, Williams, Brenda, Conscience, Aude, Durnall, Jennifer, Jackson, Steven, Costa, Magdaline, Elliott, David, Haylock, David N, Nilsson, Susan K, Saffery, Richard, Schenke-Layland, Katja, Oshlack, Alicia, Mikkola, Hanna K A, Stanley, Edouard G, Elefanty, Andrew G

“…The ability to generate hematopoietic stem cells from human pluripotent cells would enable many biomedical applications. We find that hematopoietic CD34…”
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Derivation of endothelial cells from human embryonic stem cells in fully defined medium enables identification of lysophosphatidic acid and platelet activating factor as regulators of eNOS localization by Costa, Magdaline, Sourris, Koula, Lim, Sue Mei, Yu, Qing C, Hirst, Claire E, Parkington, Helena C, Jokubaitis, Vanta J, Dear, Anthony E, Liu, Hong B, Micallef, Suzanne J, Koutsis, Kathy, Elefanty, Andrew G, Stanley, Edouard G

“…The limited availability of human vascular endothelial cells (ECs) hampers research into EC function whilst the lack of precisely defined culture conditions…”
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Expansion of human embryonic stem cells in vitro by Costa, Magdaline, Sourris, Koula, Hatzistavrou, Tanya, Elefanty, Andrew G, Stanley, Edouard G

“…This unit describes a protocol for the large-scale expansion of karyotypically normal human embryonic stem cells (hESCs). hESCs can be maintained indefinitely…”
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The hESC line Envy expresses high levels of GFP in all differentiated progeny by Stanley, Edouard G, Costa, Magdaline, Dottori, Mirella, Ng, Elizabeth, Hawes, Susan M, Sourris, Koula, Jamshidi, Pegah, Pera, Martin F, Elefanty, Andrew G

“…Human embryonic stem cells (hESCs) have been advanced as a potential source of cells for use in cell replacement therapies. The ability to identify hESCs and…”
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Generation of human embryonic stem cell reporter knock-in lines by homologous recombination by Davis, Richard P, Grandela, Catarina, Sourris, Koula, Hatzistavrou, Tanya, Dottori, Mirella, Elefanty, Andrew G, Stanley, Edouard G, Costa, Magdaline

“…This unit describes a series of technical procedures to form clonal human embryonic stem cell (hESC) lines that are genetically modified by homologous…”
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Overexpression of a dominant negative CREB protein in HT-1080 cells selectively disrupts plasminogen activator inhibitor type 2 but not tissue-type plasminogen activator gene expression by Costa, Magdaline, Shen, Yang, Medcalf, Robert L

“…The tissue-type plasminogen activator (t-PA) and plasminogen activator inhibitor type 2 (PAI-2) genes are differentially regulated by 12-phorbol 13-myristate…”
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Urokinase-mediated transactivation of the plasminogen activator inhibitor type 2 ( PAI-2) gene promoter in HT-1080 cells utilises AP-1 binding sites and potentiates phorbol ester-mediated induction of endogenous PAI-2 mRNA by Dear, Anthony E, Costa, Magdaline, Medcalf, Robert L

“…Urokinase-type plasminogen activator (u-PA) bound to its receptor, u-PAR, initiates signal transduction pathways able to induce expression of the activator…”
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Transcriptional regulation of the tissue‐type plasminogen‐activator gene in human endothelial cells : identification of nuclear factors that recognise functional elements in the tissue‐type plasminogen‐activator gene promoter by Costa, Magdaline, Shen, Yang, Maurer, Fabienne, Medcalf, Robert L.

“…The gene encoding human tissue‐type plasminogen activator (t‐PA) is regulated in a cell‐type‐specific manner. Previous studies in non‐endothelial cells have…”
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Ectopic expression of the cAMP‐responsive element binding protein inhibits phorbol ester‐mediated induction of tissue‐type plasminogen activator gene expression by Costa, Magdaline, Medcalf, Robert L.

“…The human tissue‐type plasminogen activator (t‐PA) gene is regulated in a cell‐type dependent manner. The t‐PA gene is transcriptionally induced by the phorbol…”
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