Method validation for the determination of total mercury in fish muscle by cold vapour atomic absorption spectrometry

A method was validated for the determination of total Hg in fish muscle using continuous flow cold vapour atomic absorption (CVAAS) after microwave digestion in closed vessels. The method was validated according to European Union Regulations 333/2007 and 657/2002, considering the maximum level for t...

Full description

Saved in:

Bibliographic Details
Published in:	Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment Vol. 29; no. 4; pp. 617 - 624
Main Authors:	Nascimento Neto, A.P, Costa, L.C.S, Kikuchi, A.N.S, Furtado, D.M.S, Araújo, M.Q, Melo, M.C.C
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis Group 01-04-2012 Taylor & Francis Ltd
Subjects:	Animals ANÁLISE QUÍMICA Brazil CARNE CHEMICAL COMPOSITION Chemical compounds COMPOSICIA³N QUIMICA COMPOSITION CHIMIQUE CONTAMINACIA³N CONTAMINATION CONTAMINAÇÃO FISH Fishes Food Contamination - analysis Food Contamination - statistics & numerical data HEAVY METALS heavy metals - mercury http://www.fao.org/aos/agrovoc#c_15903 http://www.fao.org/aos/agrovoc#c_1794 http://www.fao.org/aos/agrovoc#c_2223 http://www.fao.org/aos/agrovoc#c_28317 http://www.fao.org/aos/agrovoc#c_4253 http://www.fao.org/aos/agrovoc#c_4669 http://www.fao.org/aos/agrovoc#c_4751 LA©GISLATION LEGISLACIA³N LEGISLATION LEGISLAÇÃO Limit of Detection MA©TAL LOURD MEAT Meat - analysis MERCURE MERCURIO MERCURY Mercury - analysis MERCÚRIO METAL PESADO METALES PESADOS metals analysis - AAS Microwaves Muscles - chemistry PEIXE PESCADO POISSON (ALIMENT) Seafood - analysis Spectrophotometry, Atomic - methods Spectrum analysis Uncertainty Validation studies VIANDE Brazil
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	A method was validated for the determination of total Hg in fish muscle using continuous flow cold vapour atomic absorption (CVAAS) after microwave digestion in closed vessels. The method was validated according to European Union Regulations 333/2007 and 657/2002, considering the maximum level for the metal in fish, established by European Union regulation 1881/2006. The procedure for determining linear range, selectivity, recovery, precision, trueness, decision limit (CCα), detection capability (CCβ), measurement uncertainty and robustness of the method is reported. The results of the validation process demonstrate the method fulfils the provisions of the Commission Regulation. The selectivity study indicated that there was no matrix effect on the calibration curve between the concentration range of 1.0 and 30.0 µg Hg l −1 . The mean recovery calculated at six levels of fortification was in the range of 94-104%. The limit of detection (LOD) and limit of quantification (LOQ) values were 4.90 and 15.7 µg kg −1 , while the CCα and CCβ values were 0.517 and 0.533 mg kg −1 , respectively, for the maximum contaminant level of 0.500 mg kg −1 . The relative expanded measurement uncertainty of the method was 0.055 mg kg −1 . The method was not affected by slight variations of some critical factors (ruggedness minor changes) as sample mass and volume of the HNO 3 and H 2 O 2 used in the digestion step. The method allowed accurate confirmation analyses of the CRM DORM 3®. In fact, the Z-scores attained in a proficiency test round were well below the reference value of 2.0, proving the excellent performance of the laboratory.
Bibliography:	Q03 D50 2012003389 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1944-0049 1944-0057
DOI:	10.1080/19440049.2011.642009