Genomic characterization of BKC-1–producing Klebsiella pneumoniae strain belonging to high-risk clone sequence type 11 isolated from a river in Brazil

Carbapenem-resistant Klebsiella pneumoniae is a common cause of healthcare-related infections, and it is widespread in hospitals and diverse environments with potentially serious public health implications. Herein, we have reported the isolation and characterization of an environmental Brazilian Kle...

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Bibliographic Details
Published in:	The Science of the total environment Vol. 850; p. 157917
Main Authors:	Lobato, Amalia, Souza, Cintya O., Martins, Willames M.B.S., Barata, Rafael R., Camargo, Daniel S., Dutra, Lívia M.G., Carneiro, Irna C.R.S., Costa, Clevia J.S., Brasiliense, Danielle M.
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 01-12-2022
Subjects:	Amazon region Environment pollution Extensively drug-resistant phenotype K. pneumoniae Resistance genes K. pneumoniae Extensively drug-resistant phenotype Environment pollution Amazon region Resistance genes
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Summary:	Carbapenem-resistant Klebsiella pneumoniae is a common cause of healthcare-related infections, and it is widespread in hospitals and diverse environments with potentially serious public health implications. Herein, we have reported the isolation and characterization of an environmental Brazilian Klebsiella carbapenemase (BKC-1)–producing K. pneumoniae strain (IEC1205) isolated in 2018 from a river in the Amazon region, Brazil. Antimicrobial susceptibility of this strain was evaluated by broth microdilution and demonstrated resistance to several antibiotics including β-lactams, aminoglycosides, fluoroquinolones, and polymyxins. It has an extensively drug-resistant phenotype. Genomic analysis revealed that IEC1205 belonged to sequence type 11, clonal complex 258 and the presence of blaBKC-1 and two other β-lactamase–encoding genes (blaCTX-M-15 and blaSHV-11). The predicted virulence was associated with biofilm formation–related genes, a type VI secretion system, siderophore production, and type I and II fimbriae formation. We have identified an IncQ1 plasmid, named pIEC1205, harboring blaBKC-1 with high similarity to previously described plasmids carrying blaBKC-1 and blaBKC-2 genes. To our knowledge, this is the first report of an environmental BKC-1–producing K. pneumoniae strain. [Display omitted] •This is the first report of an environmental BKC-1–producing K. pneumoniae.•IEC1205 strain has an extensively drug-resistant phenotype.•K. pneumoniae IEC1205 strain belongs to the ST11/CC258 high-risk clone.•Less prevalent carbapenemases should be included in the surveillance protocols.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0048-9697 1879-1026
DOI:	10.1016/j.scitotenv.2022.157917