FRET detection in the plasma membrane using Total Internal Reflection Fluorescence Lifetime Imaging Microscopy

We developed a total internal reflection fluorescence lifetime imaging microscope to perform functional imaging of living cells membranes labeled with FRET couples. Forster resonance energy transfer efficiency can thus be followed with subwavelength axial resolution.

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Bibliographic Details
Published in:	2008 Conference on Lasers and Electro-Optics and 2008 Conference on Quantum Electronics and Laser Science pp. 1 - 2
Main Authors:	Blandin, P., Leveque-Fort, S., Lecart, S., Druon, F., Georges, P., Cossec, J.C., Potier, M.C., Lenkei, Z.
Format:	Conference Proceeding
Language:	English
Published:	IEEE 01-05-2008
Subjects:	(170.6920) Time resolved imaging (180.2520) Fluorescence microscopy Biomedical measurements Biomedical optical imaging Biomembranes Fluorescence Image resolution Microscopy Optical filters Optical imaging Optical reflection Plasmas
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Description
Summary:	We developed a total internal reflection fluorescence lifetime imaging microscope to perform functional imaging of living cells membranes labeled with FRET couples. Forster resonance energy transfer efficiency can thus be followed with subwavelength axial resolution.
ISBN:	1557528594 9781557528599
ISSN:	2160-9047
DOI:	10.1109/CLEO.2008.4551061