Inactivation of duck hepatitis B virus by a hydrogen peroxide gas plasma sterilization system: laboratory and ‘in use’ testing

Inactivation of duck hepatitis B virus by a hydrogen peroxide gas plasma sterilization system: laboratory and ‘in use’ testing

Human hepatitis B virus (HBV) is an important cause of nosocomial infections and can be transmitted by contaminated instruments. However, tests of the efficacy of sterilization of materials and equipment contaminated by HBV are difficult to perform because the virus cannot be cultured in the laborat...

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Bibliographic Details
Published in:The Journal of hospital infection Vol. 41; no. 4; pp. 317 - 322
Main Authors: Vickery, K., Deva, A.K., Zou, J., Kumaradeva, P., Cossart, L.Bissett and Y.E.
Format: Journal Article
Language:English
Published: Kent Elsevier Ltd 01-04-1999
Elsevier
Subjects:
Animals
Biological and medical sciences
Cross Infection - prevention & control
DHBV
Disinfectants
Duck hepatitis B virus
Ducks
Equipment Contamination
gas plasma
General aspects
Hepadnaviridae Infections - prevention & control
Hepadnaviridae Infections - transmission
hepadnavirus
Hepatitis B Virus, Duck - isolation & purification
Hepatitis B Virus, Duck - physiology
Hydrogen Peroxide
Infectious diseases
Medical sciences
Sterilization
Sterilization - methods
Viral diseases
hepadnavirus
gas plasma
Sterilization
hydrogen peroxide
DHBV
Duck hepatitis B virus
Medical equipment
Plasma
Laparoscopy
Method
Experimental study
Inactivation
Hydrogen Peroxides
Virus
Avihepadnavirus
Hepadnaviridae
Hygiene
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Summary:Human hepatitis B virus (HBV) is an important cause of nosocomial infections and can be transmitted by contaminated instruments. However, tests of the efficacy of sterilization of materials and equipment contaminated by HBV are difficult to perform because the virus cannot be cultured in the laboratory. In this study, we aimed to evaluate the capability of a low temperature, hydrogen peroxide gas plasma sterilizer (Sterrad®, Advanced Sterilization Products, Irvine California,) to inactivate duck hepatitis B virus (DHBV). In laboratory efficacy studies using DHBV dried on to glass filter carriers and exposed to one-half of the hydrogen peroxide gas plasma sterilization process, there was a 107or greater decrease in the viral titer, with no infectivity detected on the carriers after treatment. In-use studies were performed using a laparoscope that was experimentally contaminated with DHBV to mimic the possible transmission of infection between successive patients. Following exposure to the hydrogen peroxide gas plasma sterilization process no transmission of DHBV infection from the laparoscope occurred despite obvious visual soiling with blood (N=8) while the transmission rate for the unprocessed laparoscope (positive control) was 100% (26/26), and that for instruments after a water wash was 63% (7/11). In conclusion the hydrogen gas plasma sterilization process completely inactivates DHBV a representative of the hepadna group of viruses.
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ISSN:0195-6701
1532-2939
DOI:10.1053/jhin.1998.0516