Search Results - "Cooperman, S S"

Site-Specific Fluorescent Labeling of RNA Interior Positions by Cooperman, Barry S

“…The introduction of fluorophores into RNA for both in vitro and in cellulo studies of RNA function and cellular distribution is a subject of great current…”
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Engine out of the chassis: Cell-free protein synthesis and its uses by Rosenblum, Gabriel, Cooperman, Barry S.

“…The translation machinery is the engine of life. Extracting the cytoplasmic milieu from a cell affords a lysate capable of producing proteins in concentrations…”
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Human mitochondria require mtRF1 for translation termination at non-canonical stop codons by Krüger, Annika, Remes, Cristina, Shiriaev, Dmitrii Igorevich, Liu, Yong, Spåhr, Henrik, Wibom, Rolf, Atanassov, Ilian, Nguyen, Minh Duc, Cooperman, Barry S., Rorbach, Joanna

“…The mitochondrial translation machinery highly diverged from its bacterial counterpart. This includes deviation from the universal genetic code, with AGA and…”
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Ataluren binds to multiple protein synthesis apparatus sites and competitively inhibits release factor-dependent termination by Huang, Shijie, Bhattacharya, Arpan, Ghelfi, Mikel D., Li, Hong, Fritsch, Clark, Chenoweth, David M., Goldman, Yale E., Cooperman, Barry S.

“…Genetic diseases are often caused by nonsense mutations, but only one TRID (translation readthrough inducing drug), ataluren, has been approved for clinical…”
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Dynamics of translation by single ribosomes through mRNA secondary structures by Chen, Chunlai, Zhang, Haibo, Broitman, Steven L, Reiche, Michael, Farrell, Ian, Cooperman, Barry S, Goldman, Yale E

“…The secondary structure of mRNAs can slow or even halt protein synthesis. Single-molecule FRET studies of distinct steps in the translation elongation cycle…”
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Single-Molecule Fluorescence Measurements of Ribosomal Translocation Dynamics by Chen, Chunlai, Stevens, Benjamin, Kaur, Jaskarin, Cabral, Diana, Liu, Hanqing, Wang, Yuhong, Zhang, Haibo, Rosenblum, Gabriel, Smilansky, Zeev, Goldman, Yale E., Cooperman, Barry S.

“…We employ single-molecule fluorescence resonance energy transfer (smFRET) to study structural dynamics over the first two elongation cycles of protein…”
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Theory and practice to conserve freshwater biodiversity in the Anthropocene by Flitcroft, Rebecca, Cooperman, Michael S., Harrison, Ian J., Juffe‐Bignoli, Diego, Boon, Philip J.

“…The unprecedented impact of human activities on nature has led scientists to propose we might now be in a new geological epoch: the Anthropocene. Significant…”
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Kinetically Competent Intermediates in the Translocation Step of Protein Synthesis by Pan, Dongli, Kirillov, Stanislav V., Cooperman, Barry S.

“…Translocation requires large-scale movements of ribosome-bound tRNAs. Using tRNAs that are proflavin labeled and single-turnover rapid kinetics assays, we…”
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Kinetics of initiating polypeptide elongation in an IRES-dependent system by Zhang, Haibo, Ng, Martin Y, Chen, Yuanwei, Cooperman, Barry S

“…The intergenic IRES of Cricket Paralysis Virus (CrPV-IRES) forms a tight complex with 80S ribosomes capable of initiating the cell-free synthesis of complete…”
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Allosteric vs. spontaneous exit-site (E-site) tRNA dissociation early in protein synthesis by Chen, Chunlai, Stevens, Benjamin, Kaur, Jaskiran, Smilansky, Zeev, Cooperman, Barry S, Goldman, Yale E

“…During protein synthesis, deacylated transfer RNAs leave the ribosome via an exit (E) site after mRNA translocation. How the ribosome regulates tRNA…”
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A High-Throughput Assay for In Vitro Determination of Release Factor-Dependent Peptide Release from a Pretermination Complex by Fluorescence Anisotropy-Application to Nonsense Suppressor Screening and Mechanistic Studies by Ghelfi, Mikel D, Bhat, Saleem Y, Li, Hong, Cooperman, Barry S

“…Premature termination codons (PTCs) account for ~12% of all human disease mutations. Translation readthrough-inducing drugs (TRIDs) are prominent among the…”
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Fluorescent labeling of tRNA dihydrouridine residues: Mechanism and distribution by Kaur, Jaskiran, Raj, Monika, Cooperman, Barry S

“…Dihydrouridine (DHU) positions within tRNAs have long been used as sites to covalently attach fluorophores, by virtue of their unique chemical reactivity…”
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conserved protein EF4 (LepA) modulates the elongation cycle of protein synthesis by Liu, Hanqing, Chen, Chunlai, Zhang, Haibo, Kaur, Jaskiran, Goldman, Yale E, Cooperman, Barry S

“…EF4 (LepA), a strongly conserved protein, is important for bacterial growth and functional protein biosynthesis under certain conditions and is quite similar…”
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Quantitative single cell monitoring of protein synthesis at subcellular resolution using fluorescently labeled tRNA by Barhoom, Sima, Kaur, Jaskiran, Cooperman, Barry S., Smorodinsky, Nechama I., Smilansky, Zeev, Ehrlich, Marcelo, Elroy-Stein, Orna

“…We have developed a novel technique of using fluorescent tRNA for translation monitoring (FtTM). FtTM enables the identification and monitoring of active…”
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Stringent Nucleotide Recognition by the Ribosome at the Middle Codon Position by Liu, Wei, Shin, Dongwon, Ng, Martin, Sanbonmatsu, Karissa Y, Tor, Yitzhak, Cooperman, Barry S

“…Accurate translation of the genetic code depends on mRNA:tRNA codon:anticodon base pairing. Here we exploit an emissive, isosteric adenosine surrogate that…”
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Microcytic anemia, erythropoietic protoporphyria, and neurodegeneration in mice with targeted deletion of iron-regulatory protein 2 by Cooperman, Sharon S., Meyron-Holtz, Esther G., Olivierre-Wilson, Hayden, Ghosh, Manik C., McConnell, Joseph P., Rouault, Tracey A.

“…Iron-regulatory proteins (IRP14144) 1 and 2 posttranscriptionally regulate expression of transferrin receptor (TfR), ferritin, and other iron metabolism…”
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Robotic-Assisted Total Knee Arthroplasty in Obese Patients by Richardson, Mary K., DiGiovanni, Ryan M., McCrae, Brian K., Cooperman, Wesley S., Ludington, John, Heckmann, Nathanael D., Oakes, Daniel A.

“…Robotic-assisted systems have gained popularity in total knee arthroplasty (TKA). The purpose of this study was to evaluate operative characteristics and…”
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Role of hybrid tRNA-binding states in ribosomal translocation by Walker, Sarah E, Shoji, Shinichiro, Pan, Dongli, Cooperman, Barry S, Fredrick, Kurt

“…During translation, tRNAs must move rapidly to their adjacent sites in the ribosome while maintaining precise pairing with mRNA. This movement (translocation)…”
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Real-time assay for testing components of protein synthesis by Rosenblum, Gabriel, Chen, Chunlai, Kaur, Jaskiran, Cui, Xiaonan, Goldman, Yale E, Cooperman, Barry S

“…We present a flexible, real-time-coupled transcription-translation assay that involves the continuous monitoring of fluorescent Emerald GFP formation. Along…”
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A Quantitative Kinetic Scheme for 70 S Translation Initiation Complex Formation by Grigoriadou, Christina, Marzi, Stefano, Kirillov, Stanislas, Gualerzi, Claudio O., Cooperman, Barry S.

“…Association of the 30 S initiation complex (30SIC) and the 50 S ribosomal subunit, leading to formation of the 70 S initiation complex (70SIC), is a critical…”
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