Lipid composition of the extracellular matrix of Botrytis cinerea germlings

Six simple lipid classes (mono-, di- and tri-acylglycerols, free fatty acids, free fatty alcohols and wax esters) were identified by TLC in the extracellular matrix of Botrytis cinerea germlings and the molecular components of each class were characterized using GC-MS. The relative amounts of fatty...

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Bibliographic Details
Published in:	Phytochemistry (Oxford) Vol. 53; no. 2; pp. 293 - 298
Main Authors:	Cooper, Lol L.D., Oliver, James E., De Vilbiss, E.David, Doss, Robert P.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier Ltd 2000 Elsevier
Subjects:	Biological and medical sciences Botrytis - chemistry Botrytis - growth & development Botrytis cinerea Characterization Diglycerides - analysis Esters - analysis Extracellular matrix Extracellular Matrix - chemistry Fatty acids Fatty Acids, Nonesterified - analysis Fatty alcohols Fatty Alcohols - analysis Fundamental and applied biological sciences. Psychology Fungal plant pathogens Glycerides - analysis Gray mold Lipids Lipids - analysis Microbiology Morphology, structure, chemical composition Mycology Phytopathology. Animal pests. Plant and forest protection Scelerotiniaceae Systematics. Morphology. Development cycle. Physiology Triglycerides - analysis Waxes - analysis Characterization Scelerotiniaceae Botrytis cinerea Extracellular matrix Lipids Fatty alcohols Gray mold Fatty acids Fungi Plant pathogen Fatty alcohol Sporulation Chemical composition Fungi Imperfecti Thallophyta
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Summary:	Six simple lipid classes (mono-, di- and tri-acylglycerols, free fatty acids, free fatty alcohols and wax esters) were identified by TLC in the extracellular matrix of Botrytis cinerea germlings and the molecular components of each class were characterized using GC-MS. The relative amounts of fatty acids and fatty alcohols within each lipid class were determined by GC-FID. Over all the lipid classes, the most abundant saturated fatty acids were palmitic (ca. 30%) and stearic acid (ca. 22%). Palmitoleic and oleic acids made up ca. 21% and 24% (respectively) of the free fatty acids, while erucic (ca. 4.1%) and linoleic (ca. 3.6%) acids were the most abundant unsaturated fatty acids in the acylglycerides. The acylglycerides also contained almost 35% long chain fatty acids (C20:0 to C28:0). Six fatty acids were identified which had odd-numbered carbon chain lengths (C15:0, C17:0, C19:0, C21:0, C23:0 and C25:0). Of these, pentacosanoic acid made up almost 14% of the fatty acids in the acylglycerides. Three methyl-branched chain fatty acids, namely isopalmitic, isoheptadecanoic and anteisopalmitic, were identified in the ECM, all in small amounts. Of the fatty alcohols identified, only palmityl and stearyl alcohols were found in the free form (ca. 57% and 43%, respectively) but arachidyl alcohol (ca. 47%) and 1-octacosanol (ca. 30%) were the most abundant fatty alcohols found in the wax ester fraction.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0031-9422 1873-3700
DOI:	10.1016/S0031-9422(99)00495-1