Capping protein is essential for cell migration in vivo and for filopodial morphology and dynamics

Capping protein is essential for cell migration in vivo and for filopodial morphology and dynamics

Capping protein (CP) binds to barbed ends of growing actin filaments and inhibits elongation. CP is essential for actin-based motility in cell-free systems and in Dictyostelium. Even though CP is believed to be critical for creating the lamellipodial actin structure necessary for protrusion and migr...

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Bibliographic Details
Published in:Molecular biology of the cell Vol. 25; no. 14; pp. 2152 - 2160
Main Authors: Sinnar, Shamim A, Antoku, Susumu, Saffin, Jean-Michel, Cooper, Jon A, Halpain, Shelley
Format: Journal Article
Language:English
Published: United States The American Society for Cell Biology 15-07-2014
Subjects:
Actins - metabolism
Animals
CapZ Actin Capping Protein - physiology
Cell Line, Tumor
Cell Movement
Cell Shape
Gene Knockdown Techniques
Mice
Pseudopodia - metabolism
Pseudopodia - ultrastructure
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Summary:Capping protein (CP) binds to barbed ends of growing actin filaments and inhibits elongation. CP is essential for actin-based motility in cell-free systems and in Dictyostelium. Even though CP is believed to be critical for creating the lamellipodial actin structure necessary for protrusion and migration, CP's role in mammalian cell migration has not been directly tested. Moreover, recent studies have suggested that structures besides lamellipodia, including lamella and filopodia, may have unappreciated roles in cell migration. CP has been postulated to be absent from filopodia, and thus its role in filopodial activity has remained unexplored. We report that silencing CP in both cultured mammalian B16F10 cells and in neurons of developing neocortex impaired cell migration. Moreover, we unexpectedly observed that low levels of CP were detectable in the majority of filopodia. CP depletion decreased filopodial length, altered filopodial shape, and reduced filopodial dynamics. Our results support an expansion of the potential roles that CP plays in cell motility by implicating CP in filopodia as well as in lamellipodia, both of which are important for locomotion in many types of migrating cells.
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content type line 23
ISSN:1059-1524
1939-4586
DOI:10.1091/mbc.e13-12-0749