Search Results - "Connor, Katherine R."

Protein purification via temperature-dependent, intein-mediated cleavage from an immobilized metal affinity resin by Mills, Kenneth V., Connor, Katherine R., Dorval, Deirdre M., Lewandowski, Katherine T.

“…The intein that interrupts the DNA polymerase II DP2 subunit in Pyrococcus abyssi can be overexpressed in Escherichia coli and purified as an unspliced…”
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The structure, regulation and activity of non‐canonical inteins by Mills, Kenneth V., Reitter, Julie N., Connor, Katherine R., Nicastri, Michael C., Williams, Jennie E., Colelli, Kathryn M., Marieni, Michelle D.

“…Abstract only Protein splicing is the post‐translational excision of an intervening polypeptide (intein) from its flanking domains (the exteins), concomitant…”
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Splicing of a non‐canonical class three intein from Clostridium thermocellum by Pusztay, Jennifer M., Drago, Matthew J., Powers, Taryn L., Schufreider, Ann K., Connor, Katherine R., Reitter, Julie N., Mills, Kenneth V.

“…Abstract only Protein splicing is a post‐translational event by which an intervening polypeptide, called an intein, facilitates its own excision from the…”
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Non‐canonical inteins: Alternate mechanisms for protein splicing by Mills, Kenneth V., Powers, Taryn L., Drago, Matthew J., Connor, Katherine R., Reitter, Julie N.

“…Protein splicing involves the post‐translational excision of an intervening polypeptide (the intein) from flanking domains (the exteins), as well as ligation…”
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Non‐canonical inteins: Protein splicing by alternate mechanisms by Mills, Kenneth V., Reitter, Julie N., Connor, Katherine R., Nicastri, Michael C., Urbanski, Laura M., Williams, Jennie E., Colelli, Kathryn M., Savidis, George, Marieni, Michelle D.

“…Abstract only Protein splicing is the post‐translational excision of an intervening polypeptide (intein) from its flanking domains (the exteins), concomitant…”
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Manipulation of protein splicing side‐reactions to facilitate protein purification and expressed protein ligation by Nadelson, Adam C., York, Daniel J., Dorval, Deirdre M., Lewandowski, Katherine T., Connor, Katherine R., Reitter, Julie N., Mills, Kenneth V.

“…The PolII intein from Pyrococcus abyssi can be modified by mutation to facilitate cleavage of either the flanking N‐extein or C‐extein in a temperature…”
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Catalysis of individual steps of protein splicing of the Pyrococcus abyssi PolII intein by Dorval, Deirdre M., Connor, Katherine R., Mills, Kenneth V.

“…Protein splicing involves the excision of an intervening polypeptide, the intein, from flanking polypeptides, the exteins, concomitant with the specific…”
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Protein splicing of a non‐canonical Clostridium thermocellum intein with N‐terminal Gln by Powers, Taryn L, Drago, Matthew J., Dorval, Deirdre M., Connor, Katherine R., Mills, Kenneth V.

“…Protein splicing is a post‐translational modification by which an intervening polypeptide, called an intein, directs both its own excision from the flanking…”
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