Progeny Analysis of Glyphosate Selected Transgenic Soybeans Derived from Agrobacterium‐Mediated Transformation

Progeny Analysis of Glyphosate Selected Transgenic Soybeans Derived from Agrobacterium‐Mediated Transformation

Glyphosate selection has been implemented into an Agrobacterium‐mediated transformation system for soybean [Glycine max (L.) Merr.]. Cotyledonary explants were excised from 5‐d‐old seedlings and inoculated with an Agrobacterium strain carrying the CP4 gene, which confers tolerance to the herbicide R...

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Published in:Crop science Vol. 40; no. 3; pp. 797 - 803
Main Authors: Clemente, Thomas E., LaVallee, Bradley J., Howe, Arlene R., Conner‐Ward, Dannette, Rozman, Renee J., Hunter, Priscilla E., Broyles, Debra L., Kasten, Daniel S., Hinchee, Maud A.
Format: Journal Article
Language:English
Published: Madison Crop Science Society of America 01-05-2000
American Society of Agronomy
Subjects:
Adaptation to environment and cultivation conditions
Agrobacterium
Agrobacterium tumefaciens
Agronomy. Soil science and plant productions
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genetic aspects
Genetically modified plants
Genetics
Genetics and breeding of economic plants
Glycine max
Glyphosate
Herbicides
Seedlings
Soybean
Soybeans
Varietal selection. Specialized plant breeding, plant breeding aims
Pesticides
Systemic
Genetic determinism
Glycine max
Glyphosate
Herbicide
Grain legume
Agrobacterium
Leguminosae
Aminoacid
Dicotyledones
Angiospermae
Genetic improvement
Bacteria
Spermatophyta
Transgenic plant
Rhizobiaceae
Pesticide resistance
Gram negative bacteria
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Summary:Glyphosate selection has been implemented into an Agrobacterium‐mediated transformation system for soybean [Glycine max (L.) Merr.]. Cotyledonary explants were excised from 5‐d‐old seedlings and inoculated with an Agrobacterium strain carrying the CP4 gene, which confers tolerance to the herbicide Roundup, and the β‐glucuronidase (GUS) gene. Explants were selected on sublethal levels of glyphosate during shoot initiation and elongation. Glyphosate tolerant shoots were identifiable after 8 to 12 wk of selection. Coexpression of GUS occurred in approximately 80% of the primary transformants (R0). The R0 plants were fully fertile, with no apparent phenotypic abnormalities. Progeny analysis from 81 independent transgenic soybean events derived from glyphosate selection demonstrated that the majority of the transformants transmitted the transgenes as a single functional locus.
Bibliography:ObjectType-Article-2
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content type line 23
ISSN:0011-183X
1435-0653
DOI:10.2135/cropsci2000.403797x