Exploring the antifungal, antibiofilm and antienzymatic potential of Rottlerin in an in vitro and in vivo approach

Candida species have been responsible for a high number of invasive infections worldwide. In this sense, Rottlerin has demonstrated a wide range of pharmacological activities. Therefore, this study aimed to evaluate the antifungal, antibiofilm and antivirulence activity of Rottlerin in vitro against...

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Bibliographic Details
Published in:	Scientific reports Vol. 14; no. 1; p. 11132
Main Authors:	Silva, Nagela Bernadelli Sousa, Menezes, Ralciane Paula, Gonçalves, Daniela Silva, Santiago, Mariana Brentini, Conejo, Noemi Chagas, Souza, Sara Lemes, Santos, Anna Lívia Oliveira, da Silva, Robinson Sabino, Ramos, Salvador Boccaletti, Ferro, Eloisa Amália Vieira, Martins, Carlos Henrique Gomes
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 15-05-2024 Nature Publishing Group Nature Portfolio
Subjects:	631/326/193 631/326/22 631/326/2522 Acetophenones - pharmacology Animals Antibiofilm Antifungal activity Antifungal Agents - pharmacology Benzopyrans - pharmacology Biofilms Biofilms - drug effects Caenorhabditis elegans Caenorhabditis elegans - drug effects Candida Candida - drug effects Candida albicans - drug effects Candidiasis - drug therapy Cell viability Filamentation Humanities and Social Sciences Hydrolytic enzymes Larvae Microbial Sensitivity Tests multidisciplinary Rottlerin Scanning electron microscopy Science Science (multidisciplinary) Toxicity Yeasts Antifungal activity Antibiofilm Rottlerin Hydrolytic enzymes Caenorhabditis elegans
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Summary:	Candida species have been responsible for a high number of invasive infections worldwide. In this sense, Rottlerin has demonstrated a wide range of pharmacological activities. Therefore, this study aimed to evaluate the antifungal, antibiofilm and antivirulence activity of Rottlerin in vitro against Candida spp. and its toxicity and antifungal activity in vivo. Rottlerin showed antifungal activity against all yeasts evaluated, presenting Minimum Inhibitory and Fungicidal Concentration (MIC and MFC) values of 7.81 to > 1000 µg/mL. Futhermore, it was able to significantly inhibit biofilm production, presenting Biofilm Inhibitory Concentration (MICB 50 ) values that ranged from 15.62 to 250 µg/mL and inhibition of the cell viability of the biofilm by 50% (IC 50 ) from 2.24 to 12.76 µg/mL. There was a considerable reduction in all hydrolytic enzymes evaluated, with emphasis on hemolysin where Rottlerin showed a reduction of up to 20%. In the scanning electron microscopy (SEM) analysis, Rottlerin was able to completely inhibit filamentation by C. albicans . Regarding in vivo tests, Rottlerin did not demonstrate toxicity at the therapeutic concentrations demonstrated here and was able to increase the survival of C. elegans larvae infected. The results herein presented are innovative and pioneering in terms of Rottlerin’s multipotentiality against these fungal infections.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-024-61179-z