Coronamycins, peptide antibiotics produced by a verticillate Streptomyces sp. (MSU-2110) endophytic on Monstera sp

1 Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA 2 Department of Integrated Biology, Brigham Young University, Provo, UT 84602, USA 3 Department of Microbiology, Brigham Young University, Provo, UT 84602, USA 4 Department of Neurology, Harvard Medical School, and Cent...

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Published in:	Microbiology (Society for General Microbiology) Vol. 150; no. 4; pp. 785 - 793
Main Authors:	Ezra, David, Castillo, Uvidelio F, Strobel, Gary A, Hess, Wilford M, Porter, Heidi, Jensen, James B, Condron, Margaret A. M, Teplow, David B, Sears, Joseph, Maranta, Michelle, Hunter, Michelle, Weber, Barbara, Yaver, Debbie
Format:	Journal Article
Language:	English
Published:	Reading Soc General Microbiol 01-04-2004 Society for General Microbiology
Subjects:	Animals Anti-Bacterial Agents - biosynthesis Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibiotics (antibacterial agents, antifungal agents) Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents Applied microbiology Araceae - microbiology Araceae - ultrastructure Bacteria - drug effects Biological and medical sciences Cell Line Fundamental and applied biological sciences. Psychology Fungi - drug effects Humans Microbial Sensitivity Tests Microbiology Microscopy, Electron, Scanning Molecular Sequence Data Parasitic Sensitivity Tests Peptides Plasmodium falciparum - drug effects Sequence Analysis, DNA Spores, Fungal - ultrastructure Streptomyces Streptomyces - metabolism Streptomyces - ultrastructure South America Peru America Protozoal disease Yeast Cryptococcus neoformans Peptides HPLC chromatography Actinomycetales Fungi Plasmodium falciparum Bacteria Fungi Imperfecti Thallophyta Protozoa Apicomplexa Streptomycetaceae Bioassay Malaria Streptomyces Parasitosis Fermentation Infection Sulfur containing aminoacid Antibiotic Antiseptic Actinomycetes Yield
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Summary:	1 Department of Plant Sciences, Montana State University, Bozeman, MT 59717, USA 2 Department of Integrated Biology, Brigham Young University, Provo, UT 84602, USA 3 Department of Microbiology, Brigham Young University, Provo, UT 84602, USA 4 Department of Neurology, Harvard Medical School, and Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, MA 02115, USA 5 Department of Chemistry, Montana State University, Bozeman, MT 59717, USA 6 Novozymes Biotech Inc., 1445 Drew Ave, Davis, CA 95616, USA Correspondence Gary Strobel uplgs{at}montana.edu Coronamycin is a complex of novel peptide antibiotics with activity against pythiaceous fungi and the human fungal pathogen Cryptococcus neoformans . It is also active against the malarial parasite, Plasmodium falciparum , with an IC 50 of 9·0 ng ml 1 . Coronamycin is produced by a verticillate Streptomyces sp. isolated as an endophyte from an epiphytic vine, Monstera sp., found in the Manu region of the upper Amazon of Peru. Bioassay-guided fractionation of the fermentation broths of this endophyte on silica gel and HPLC chromatography yielded two principal, inseparable, peptides with masses of 1217·9 and 1203·8 Da. Three other minor, but related components, are also present in the preparation. Amino acid analysis of coronamycin revealed residues of component 1, component 2, methionine, tyrosine and leucine at a ratio of 2 : 2 : 1 : 1 : 3. Other compounds with antifungal activities are also produced by this endophytic streptomycete. The GenBank accession number for the sequence reported in this paper is AY327845 .
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1350-0872 1465-2080
DOI:	10.1099/mic.0.26645-0