Evaluation of Multidrug Resistance of Salmonella Isolated from Pork Meat Obtained from Traditional Slaughter Systems in Romania

Evaluation of Multidrug Resistance of Salmonella Isolated from Pork Meat Obtained from Traditional Slaughter Systems in Romania

Antibiotic resistance among pathogenic bacteria in humans and animals poses a critical public health challenge, leading to diminished effectiveness of existing antimicrobial treatments. Notably, animal-derived food products are significant vectors for the transmission of resistant bacteria to humans...

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Bibliographic Details
Published in:Microorganisms (Basel) Vol. 12; no. 11; p. 2196
Main Authors: Tăbăran, Alexandra, Dan, Sorin Danel, Colobaţiu, Liora Mihaela, Mihaiu, Marian, Condor, Sergiu, Mărgăoan, Rodica, Crişan-Reget, Oana Lucia
Format: Journal Article
Language:English
Published: Basel MDPI AG 30-10-2024
MDPI
Subjects:
Animal products
Animal-based foods
Animals
Antibiotic resistance
Antibiotics
Antimicrobial agents
Antisera
Asymptomatic
Bacteria
Bacterial infections
Drug resistance
Farms
Food chains
Food contamination
Food contamination & poisoning
Food sources
Foodborne diseases
High resistance
Hogs
Illnesses
Infections
Meat
Multidrug resistance
Pathogens
Polymerase chain reaction
Pork
Public health
Risk reduction
Salmonella
Serotyping
Slaughter
Streptomycin
Sulfamethoxazole
System effectiveness
Vectors
Romania
United Kingdom > UK
United States > US
Germany
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Summary:Antibiotic resistance among pathogenic bacteria in humans and animals poses a critical public health challenge, leading to diminished effectiveness of existing antimicrobial treatments. Notably, animal-derived food products are significant vectors for the transmission of resistant bacteria to humans, with Salmonella species being predominant culprits in foodborne illnesses. This study investigates the prevalence and antibiotic resistance patterns of Salmonella serovars isolated from traditionally sourced pork meat in Romania. Over a four-year period, 208 pork samples were collected using standardized protocols during traditional slaughtering practices. Salmonella spp. were isolated following ISO 6579:2002 guidelines and confirmed using biochemical assays and PCR. Serotyping was performed using specific antisera, and antimicrobial susceptibility testing was conducted through the standard disk diffusion method, assessing 11 antibiotics. Results indicated a 23.07% prevalence of Salmonella, with 48 isolates categorized into eight serovars, primarily S. Infantis (n = 15), S. Typhimurium (n = 15), and S. Derby (n = 11). PCR results confirmed the presence of Salmonella by detecting the hilA and ompC genes, with 31.25% of isolates being positive for the Typhimurium-specific sequence. Notably, 93.75% of the isolates were multidrug-resistant (MDR), exhibiting high resistance rates against streptomycin (91.66%) (>10 µg), tetracycline (83.33%) (>30 µg), and sulfamethoxazole (68.75%) (>300 µg). More than 60% of MDR isolates displayed resistance to five or more antibiotics. These findings underscore the need for coordinated control measures in the pork production chain to combat the spread of Salmonella and protect public health. Enhanced surveillance and intervention strategies are crucial for addressing antibiotic resistance and reducing the risk of foodborne illnesses linked to contaminated animal products.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms12112196