Sleep decreases neuronal activity control of microglial dynamics in mice

Sleep decreases neuronal activity control of microglial dynamics in mice

Microglia, the brain-resident immune cells, are highly ramified with dynamic processes transiently contacting synapses. These contacts have been reported to be activity-dependent, but this has not been thoroughly studied yet, especially in physiological conditions. Here we investigate neuron-microgl...

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Bibliographic Details
Published in:Nature communications Vol. 13; no. 1; p. 6273
Main Authors: Hristovska, I., Robert, M., Combet, K., Honnorat, J., Comte, J-C, Pascual, O.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 21-10-2022
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Subjects:
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Animals
CX3CR1 protein
Dendritic spines
Homeostasis
Humanities and Social Sciences
Immune system
Mice
Microglia
Microglia - physiology
multidisciplinary
Neuroimaging
Neuronal Plasticity - physiology
Neurons - physiology
NREM sleep
Parenchyma
Science
Science (multidisciplinary)
Signal Transduction - physiology
Signaling
Sleep
Spine
Synapses
Synapses - physiology
Synaptic plasticity
Vigilance
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Summary:Microglia, the brain-resident immune cells, are highly ramified with dynamic processes transiently contacting synapses. These contacts have been reported to be activity-dependent, but this has not been thoroughly studied yet, especially in physiological conditions. Here we investigate neuron-microglia contacts and microglia morphodynamics in mice in an activity-dependent context such as the vigilance states. We report that microglial morphodynamics and microglia-spine contacts are regulated by spontaneous and evoked neuronal activity. We also found that sleep modulates microglial morphodynamics through Cx3cr1 signaling. At the synaptic level, microglial processes are attracted towards active spines during wake, and this relationship is hindered during sleep. Finally, microglial contact increases spine activity, mainly during NREM sleep. Altogether, these results indicate that microglial function at synapses is dependent on neuronal activity and the vigilance states, providing evidence that microglia could be important for synaptic homeostasis and plasticity. Microglia survey the parenchyma, which leads to morphology changes over time. Here the authors show using 2 photon imaging of microglia in vivo that sleep modulates microglial morphodynamics through Cx3cr1 signaling.
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ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-022-34035-9