P054 Effects of leucine-rich diet on inflammatory response induced by tumour growth in young, adults and senile rats

P054 Effects of leucine-rich diet on inflammatory response induced by tumour growth in young, adults and senile rats

The anorexia–cachexia syndrome is caused by metabolic changes; in particular, induced by cytokines released by tumour cells or in response to the host’s immune system. These cytokines also promote lipolysis and proteolysis. We examined the effect of leucine-rich diet on the inflammatory response in...

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Bibliographic Details
Published in:Cytokine (Philadelphia, Pa.) Vol. 59; no. 3; pp. 535 - 536
Main Authors: Colosso, J.G.D., Salomao, E.M., Gomes-Marcondes, M.C.C.
Format: Journal Article
Language:English
Published: Elsevier Ltd 01-09-2012
Subjects:
Body weight
Cachexia
Cytokines
Development
Diets
Glucose
Immune system
Inflammation
Interleukin 10
Interleukin 4
Interleukin 6
Leucine
Lipolysis
Metabolism
Proteolysis
Senile
Tumor necrosis factor
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Summary:The anorexia–cachexia syndrome is caused by metabolic changes; in particular, induced by cytokines released by tumour cells or in response to the host’s immune system. These cytokines also promote lipolysis and proteolysis. We examined the effect of leucine-rich diet on the inflammatory response in young (21 days old), adults (90days old) and senile (400days old) Walker 256 tumour-bearing rats. Wistar rats were submitted to eutrophic diet or leucine-rich diet and distributed into 7 groups: C – control rats; YW – young tumour-bearing; AW – adult tumour-bearing; SW – senile tumour-bearing; YWL – young tumour-bearing fed leucine diet; AWL – adult tumour-bearing fed leucine diet; SWL – senile tumour-bearing fed leucine diet. The tumour development induced an increase in proinflammatory cytokines. The serum tumour necrosis factor (TNF-α) content was higher in the young tumour-bearing fed leucine diet (YWL) compared to control group C and to the other groups. Another proinflammatory cytokine, interferon-γ (INF-γ) increased in the young tumour-bearing fed a leucine-rich diet group YWL vs control group C; the other groups showed similar values as the control group. The interleukin 4 (IL-4), cytokine related to defence host response showed an increase in young and senile groups compared to group C. The serum concentration of interleukin-6 (IL-6), a pro-inflammatory cytokine, increased in group YW, YWL, AWL; and SWL in relation to control group. The concentration of interleukin-1α (IL-1α), another pro-inflammatory cytokine, increased in group YW in comparison to control and the other tumour-bearing groups. The anti-inflammatory cytokine, IL-10, increased in all groups with tumour YW (728+122pg/mL); YWL (1684+339); AW (1016+61); AWL (775+140), SW (1414+178) and SWL (1445+642) vs C (313+91). The tumour/body weight ratio was lower in the young tumour-bearing groups versus senile tumour-bearing groups. The serum glucose and protein content reduced in young tumour-bearing groups compared senile tumour-bearing groups. Although the tumour growth in young rats induced chronic inflammation increasing the proinflammatory cytokines release, which occur in the development of cachexia; in senile tumour-bearing groups these effects are decreased possibly due to a reduced metabolism. Supported by PIBIC/CNPq and FAPESP #2010/11328-9; # 2010/00260-4.
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ISSN:1043-4666
1096-0023
DOI:10.1016/j.cyto.2012.06.138