Immunohistochemical Analysis of Cyclin D1 Shows Deregulated Expression in Multiple Myeloma with the t(11;14)

Immunohistochemical Analysis of Cyclin D1 Shows Deregulated Expression in Multiple Myeloma with the t(11;14)

The t(11;14)(q13;q32) chromosomal translocation, the hallmark of mantle cell lymphoma (MCL), is recurrently found in multiple myelomas (MM) by means of conventional cytogenetics. Unlike MCL, recent molecular studies of MM-derived cell lines with t(11;14) have indicated that the breakpoints are highl...

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Bibliographic Details
Published in:The American journal of pathology Vol. 156; no. 5; pp. 1505 - 1513
Main Authors: Pruneri, Giancarlo, Fabris, Sonia, Baldini, Luca, Carboni, Nadia, Zagano, Savina, Colombi, Maria Angela, Ciceri, Gabriella, Lombardi, Luigia, Rocchi, Mariano, Buffa, Roberto, Maiolo, Anna Teresa, Neri, Antonino
Format: Journal Article
Language:English
Published: Bethesda, MD Elsevier Inc 01-05-2000
ASIP
American Society for Investigative Pathology
Subjects:
Adult
Aged
Aged, 80 and over
Biological and medical sciences
Chromosomes, Human, Pair 11 - genetics
Chromosomes, Human, Pair 14 - genetics
Cyclin D1 - analysis
Cyclin D1 - genetics
Female
Hematologic and hematopoietic diseases
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Male
Medical sciences
Middle Aged
Multiple Myeloma - genetics
Multiple Myeloma - metabolism
Multiple Myeloma - pathology
Neoplasm Staging
Short Communications
Translocation, Genetic
Chromosomal aberration
Human
Immunohistochemistry
Immunopathology
Multiple
Gene overexpression
Malignant hemopathy
Cyclin D1
Gene expression
Myeloma
Carcinogenesis
Pathology
Immunoglobulinopathy
Lymphoproliferative syndrome
Cell cycle
Fluorescence in situ hybridization
Chromosome 11
Cytogenetics
Abnormal chromosome
Chromosome 14
Molecular biology
Chromosome translocation
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Summary:The t(11;14)(q13;q32) chromosomal translocation, the hallmark of mantle cell lymphoma (MCL), is recurrently found in multiple myelomas (MM) by means of conventional cytogenetics. Unlike MCL, recent molecular studies of MM-derived cell lines with t(11;14) have indicated that the breakpoints are highly dispersed over the 11q13 region; however, the fact that cyclin D1 is generally overexpressed in these cell lines suggests that this gene is the target of the translocation. To evaluate further the involvement of cyclin D1 in MM, we used immunohistochemistry and fluorescence in situ hybridization to investigate cyclin D1 expression and the presence of chromosome 11 abnormalities in a representative panel of 48 MM patients (40 at diagnosis and 8 at relapse). Cyclin D1 overexpression occurred in 12/48 (25%) of cases; combined immunohistochemistry and fluorescence in situ hybridization analyses in 39 patients showed cyclin D1 positivity in all of the cases (7/7) bearing the t(11;14), in two of the 13 cases with trisomy 11, and in one of the 19 cases with no apparent abnormalities of chromosome 11. Our data indicate that the t(11;14) translocation in MM leads to cyclin D1 overexpression and that immunohistochemical analysis may represent a reliable means of identifying this lesion in MM.
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ISSN:0002-9440
1525-2191
DOI:10.1016/S0002-9440(10)65022-5