Storage and Lyophilization of Pure Proteins

Storage and Lyophilization of Pure Proteins

This article outlines empirical procedures for the storage of pure proteins with preservation of high levels of biological activity. It describes simple and workable means of preventing microbial contamination and proteolytic degradation, and the use of various types of stabilizing additives. It set...

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 1485; p. 159
Main Authors: Ó'Fágáin, Ciarán, Colliton, Keith
Format: Journal Article
Language:English
Published: United States 2017
Subjects:
Drug Storage - methods
Drug Storage - standards
Freeze Drying - instrumentation
Freeze Drying - methods
Oxidation-Reduction
Protein Stability
Proteins - chemistry
Proteolysis
Stabilizing additives
Excipients
Modulated differential scanning calorimetry
Protein stability
Lyophilization
Freeze-drying
Freezing
Storage of pure proteins
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Summary:This article outlines empirical procedures for the storage of pure proteins with preservation of high levels of biological activity. It describes simple and workable means of preventing microbial contamination and proteolytic degradation, and the use of various types of stabilizing additives. It sets out the principles of lyophilization (otherwise known as freeze-drying, a complex process comprising freezing, primary dying, and secondary drying stages). There follows a general procedure for the use of lyophilizer apparatus with emphasis on best practice and on pitfalls to avoid. The use of modulated differential scanning calorimetry to measure the glass transition temperature, a key parameter in the design and successful operation of lyophilization processes, is described.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6412-3_9