Search Results - "Cole, Christian M."

Bioorthogonal Tetrazine-Mediated Transfer Reactions Facilitate Reaction Turnover in Nucleic Acid-Templated Detection of MicroRNA by Wu, Haoxing, Cisneros, Brandon T, Cole, Christian M, Devaraj, Neal K

“…Tetrazine ligations have proven to be a powerful bioorthogonal technique for the detection of many labeled biomolecules, but the ligating nature of these…”
Get full text

Self-reproducing catalyst drives repeated phospholipid synthesis and membrane growth by Hardy, Michael D., Yang, Jun, Selimkhanov, Jangir, Cole, Christian M., Tsimring, Lev S., Devaraj, Neal K.

“…Cell membranes are dynamic structures found in all living organisms. There have been numerous constructs that model phospholipid membranes. However, unlike…”
Get full text

In Situ Vesicle Formation by Native Chemical Ligation by Brea, Roberto J., Cole, Christian M., Devaraj, Neal K.

“…Phospholipid vesicles are of intense fundamental and practical interest, yet methods for their de novo generation from reactive precursors are limited. A…”
Get full text

Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system by Shi, Xinying, Wu, Ti, M. Cole, Christian, K. Devaraj, Neal, Joseph, Simpson

“…Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed…”
Get full text

Encapsulation of Living Cells within Giant Phospholipid Liposomes Formed by the Inverse-Emulsion Technique by Chowdhuri, Sampreeti, Cole, Christian M., Devaraj, Neal K.

“…Liposomes form spontaneously by the assimilation of phospholipids, the primary component of cell membranes. Due to their unique ability to form selectively…”
Get full text

Spontaneous Reconstitution of Functional Transmembrane Proteins During Bioorthogonal Phospholipid Membrane Synthesis by Cole, Christian M., Brea, Roberto J., Kim, Young Hun, Hardy, Michael D., Yang, Jerry, Devaraj, Neal K.

“…Transmembrane proteins are critical for signaling, transport, and metabolism, yet their reconstitution in synthetic membranes is often challenging…”
Get full text

Live-Cell Imaging of Cyclopropene Tags with Fluorogenic Tetrazine Cycloadditions by Yang, Jun, Šečkutė, Jolita, Cole, Christian M., Devaraj, Neal K.

“…Spotlight on lipids: One of the major limitations of tetrazine bioorthogonal cycloadditions is the requirement of bulky dienophile reaction partners…”
Get full text

Site-Specific Covalent Labeling of RNA by Enzymatic Transglycosylation by Alexander, Seth C, Busby, Kayla N, Cole, Christian M, Zhou, Cun Yu, Devaraj, Neal K

“…We demonstrate the site-specific incorporation of nucleo­base derivatives bearing fluoro­phores or affinity labels into a short RNA stem loop recognition motif…”
Get full text

Fluorescent Live-Cell Imaging of Metabolically Incorporated Unnatural Cyclopropene-Mannosamine Derivatives by Cole, Christian M., Yang, Jun, Šečkutė, Jolita, Devaraj, Neal K.

“…Sugar coated: We recently developed methylcyclopropenes as low‐molecular‐weight tetrazine coupling partners. Here, we demonstrate that methylcyclopropenes can…”
Get full text

In Situ Vesicle Formation by Native Chemical Ligation by Brea, Roberto J., Cole, Christian M., Devaraj, Neal K.

“…Phospholipid vesicles are of intense fundamental and practical interest, yet methods for their de novo generation from reactive precursors are limited. A…”
Get full text

In Situ Reconstitution of the Adenosine A2A Receptor in Spontaneously Formed Synthetic Liposomes by Brea, Roberto J, Cole, Christian M, Lyda, Brent R, Ye, Libin, Prosser, R. Scott, Sunahara, Roger K, Devaraj, Neal K

“…Cell transmembrane receptors play a key role in the detection of environmental stimuli and control of intracellular communication. G protein-coupled receptors…”
Get full text

Metabolically activated proteostasis regulators that protect against erastin-induced ferroptosis by Kline, Gabriel M, Madrazo, Nicole, Cole, Christian M, Pannikkat, Meera, Bollong, Michael J, Rosarda, Jessica D, Kelly, Jeffery W, Wiseman, R Luke

“…We previously showed that the proteostasis regulator compound AA147 ( -(2-hydroxy-5-methylphenyl)benzenepropanamide) potently protects against neurotoxic…”
Get full text

In Situ Reconstitution of the Adenosine A 2A Receptor in Spontaneously Formed Synthetic Liposomes by Brea, Roberto J, Cole, Christian M, Lyda, Brent R, Ye, Libin, Prosser, R Scott, Sunahara, Roger K, Devaraj, Neal K

“…Cell transmembrane receptors play a key role in the detection of environmental stimuli and control of intracellular communication. G protein-coupled receptors…”
Get full text

Spontaneous Reconstitution of Functional Transmembrane Proteins During Bioorthogonal Phospholipid Membrane Synthesis by Cole, Christian M., Brea, Roberto J., Kim, Young Hun, Hardy, Michael D., Yang, Jerry, Devaraj, Neal K.

“…Transmembrane proteins are critical for signaling, transport, and metabolism, yet their reconstitution in synthetic membranes is often challenging…”
Get full text

Live‐Cell Imaging of Cyclopropene Tags with Fluorogenic Tetrazine Cycloadditions by Yang, Jun, Šečkutė, Jolita, Cole, Christian M., Devaraj, Neal K.

Get full text

Utilization of Bioorthogonal Chemistry for Live-Cell Labeling of Glycoconjugates and the Liposomal-Based Engineering of Dynamic Artificial Cellular Models by Cole, Christian M

“…This dissertation will first explore the use of methylcyclopropene as an activated dienophile in the [4 + 2] inverse Diels–Alder cycloaddition with a tetrazine…”
Get full text

Live-Cell Imaging of Cyclopropene Tags with Fluorogenic Tetrazine Cycloadditions by Yang, Jun, Šečkutė, Jolita, Cole, Christian M., Devaraj, Neal K.

“…Lipide im Scheinwerferlicht: Eine wichtige Einschränkung bei bioorthogonalen Cycloadditionen mit Tetrazin besteht darin, sperrige dienophile Reaktionspartner…”
Get full text