Search Results - "Cold Spring Harbor protocols"

Crystal Violet Assay for Determining Viability of Cultured Cells by Feoktistova, Maria, Geserick, Peter, Leverkus, Martin

“…Adherent cells detach from cell culture plates during cell death. This characteristic can be used for the indirect quantification of cell death and to…”
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RNA Sequencing and Analysis by Kukurba, Kimberly R, Montgomery, Stephen B

“…RNA sequencing (RNA-Seq) uses the capabilities of high-throughput sequencing methods to provide insight into the transcriptome of a cell. Compared to previous…”
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Manual hematoxylin and eosin staining of mouse tissue sections by Cardiff, Robert D, Miller, Claramae H, Munn, Robert J

“…The hematoxylin and eosin (H&E) stain is the standard used for microscopic examination of tissues that have been fixed, processed, embedded, and sectioned. It…”
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Measuring Cell Death by Propidium Iodide Uptake and Flow Cytometry by Crowley, Lisa C, Scott, Adrian P, Marfell, Brooke J, Boughaba, Jeanne A, Chojnowski, Grace, Waterhouse, Nigel J

“…Propidium iodide (PI) is a small fluorescent molecule that binds to DNA but cannot passively traverse into cells that possess an intact plasma membrane. PI…”
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Illumina sequencing library preparation for highly multiplexed target capture and sequencing by Meyer, Matthias, Kircher, Martin

“…The large amount of DNA sequence data generated by high-throughput sequencing technologies often allows multiple samples to be sequenced in parallel on a…”
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Fluorescence microscopy by Sanderson, Michael J, Smith, Ian, Parker, Ian, Bootman, Martin D

“…Fluorescence microscopy is a major tool with which to monitor cell physiology. Although the concepts of fluorescence and its optical separation using filters…”
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Strategies for isolation of exosomes by Zeringer, Emily, Barta, Timothy, Li, Mu, Vlassov, Alexander V

“…Exosomes are tiny vesicles (diameter 30-150 nm) secreted by cells in culture and found in all body fluids. These vesicles, loaded with unique RNA and protein…”
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Quantitation of Apoptosis and Necrosis by Annexin V Binding, Propidium Iodide Uptake, and Flow Cytometry by Crowley, Lisa C, Marfell, Brooke J, Scott, Adrian P, Waterhouse, Nigel J

“…The surface of healthy cells is composed of lipids that are asymmetrically distributed on the inner and outer leaflet of the plasma membrane. One of these…”
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Growth and the Environment of Schizosaccharomyces pombe by Petersen, Janni, Russell, Paul

“…Here, we summarize the composition and uses of Schizosaccharomyces pombe media and discuss key issues for consideration in the generation of S. pombe cultures…”
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High-throughput illumina strand-specific RNA sequencing library preparation by Zhong, Silin, Joung, Je-Gun, Zheng, Yi, Chen, Yun-ru, Liu, Bao, Shao, Ying, Xiang, Jenny Z, Fei, Zhangjun, Giovannoni, James J

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Human cancer growth and therapy in immunodeficient mouse models by Shultz, Leonard D, Goodwin, Neal, Ishikawa, Fumihiko, Hosur, Vishnu, Lyons, Bonnie L, Greiner, Dale L

“…Since the discovery of the "nude" mouse more than 40 years ago, investigators have attempted to model human tumor growth in immunodeficient mice. Here, we…”
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DNase-seq: a high-resolution technique for mapping active gene regulatory elements across the genome from mammalian cells by Song, Lingyun, Crawford, Gregory E

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Purification of RNA using TRIzol (TRI reagent) by Rio, Donald C, Ares, Jr, Manuel, Hannon, Gregory J, Nilsen, Timothy W

“…TRIzol solubilization and extraction is a relatively recently developed general method for deproteinizing RNA. This method is particularly advantageous in…”
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IMGT/V-QUEST: IMGT standardized analysis of the immunoglobulin (IG) and T cell receptor (TR) nucleotide sequences by Giudicelli, Véronique, Brochet, Xavier, Lefranc, Marie-Paule

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Considerations for Rearing and Maintaining Anopheles in the Laboratory by Leite, Laura N, Bascuñán, Priscila, Dotson, Ellen M, Benedict, Mark Q

“…mosquitoes can transmit several human pathogens, including viruses such as o'nyong-nyong and parasites including spp. and spp., which cause malaria and…”
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Rapid Isolation of Nuclei from Cells In Vitro by Nabbi, Arash, Riabowol, Karl

“…This protocol presents a rapid, efficient, and practical (REAP) method to separate nuclei from cultured cells in vitro with as little damage and contamination…”
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Mosquito Blood Meal Analysis by Reeves, Lawrence E, Burkett-Cadena, Nathan D

“…The host associations of mosquitoes vary by species, with some species being relative generalists, whereas others specialize, to varying extents, on a…”
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Preservation of Field-Collected Mosquito Blood Meals by Reeves, Lawrence E, Burkett-Cadena, Nathan D

“…All PCR- and DNA-based blood meal analyses require host DNA from a mosquito blood meal to be effectively preserved between the time when the specimen is…”
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Extracting DNA from Preserved Mosquito Blood Meals by Reeves, Lawrence E, Burkett-Cadena, Nathan D

“…Mosquito species vary in their host associations. Although some species are relative generalists, most specialize, to varying extents, on particular types of…”
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Amplification and Identification of Vertebrate Host Cytochrome c Oxidase Subunit I ( COI ) DNA Barcoding Templates from Mosquito Blood Meals by Reeves, Lawrence E, Burkett-Cadena, Nathan D

“…Mosquitoes take blood meals from a diverse range of host animals and their host associations vary by species. Characterizing these associations is an important…”
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