Prospecting Anticancer Compounds in Actinomycetes Recovered from the Sediments of Saint Peter and Saint Paul's Archipelago, Brazil

Saint Peter and Saint Paul's Archipelago is a collection of 15 islets and rocks remotely located in the equatorial Atlantic Ocean. In this particular site, the present project intended to assess the biodiversity and biotechnological potential of bacteria from the actinomycete group. This study...

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Bibliographic Details
Published in:	Chemistry & biodiversity Vol. 13; no. 9; pp. 1149 - 1157
Main Authors:	Ferreira, Elthon G., Torres, Maria da Conceição M., da Silva, Alison B., Colares, Larissa L. F., Pires, Karine, Lotufo, Tito M. C., Silveira, Edilberto R., Pessoa, Otília D. L., Costa-Lotufo, Leticia V., Jimenez, Paula C.
Format:	Journal Article
Language:	English
Published:	Switzerland Blackwell Publishing Ltd 01-09-2016 Wiley Subscription Services, Inc
Subjects:	Actinobacteria - chemistry Actinobacteria - isolation & purification Antineoplastic Agents, Phytogenic - analysis Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - pharmacology Brazil Cell Proliferation - drug effects Cytotoxic activities Diketopiperazine Dose-Response Relationship, Drug Drug Screening Assays, Antitumor Geologic Sediments - microbiology HCT116 Cells Humans Marine microorganisms Marine natural products Molecular Conformation Piericidin Streptomyces Structure-Activity Relationship Diketopiperazine Marine microorganisms Piericidin Marine natural products Cytotoxic activities
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Summary:	Saint Peter and Saint Paul's Archipelago is a collection of 15 islets and rocks remotely located in the equatorial Atlantic Ocean. In this particular site, the present project intended to assess the biodiversity and biotechnological potential of bacteria from the actinomycete group. This study presents the first results of this assessment. From 21 sediment samples, 268 strains were isolated and codified as BRA followed by three numbers. Of those, 94 strains were grown in liquid media and submitted to chemical extractions with AcOEt (A), BuOH (B), and MeOH (M). A total of 224 extracts were screened for their cytotoxic activity and 41 were significantly active against HCT‐116 cancer cells. The obtained IC50 values ranged from 0.04 to 31.55 μg/ml. The HR‐LC/MS dereplication analysis of the active extracts showed the occurrence of several known anticancer compounds. Individual compounds, identified using HR‐MS combined with analysis of the AntiMarin database, included saliniketals A and B, piericidins A and C and glucopiericidin A, staurosporine, N‐methylstaurosporine, hydroxydimethyl‐staurosporine and N‐carbamoylstaurosporine, salinisporamycin A, and rifamycins S and B. BRA‐199, identified as Streptomyces sp., was submitted to bioassay‐guided fractionation, leading to isolation of the bioactive piericidins A and C, glucopiericidin, and three known diketopiperazines, cyclo(l‐Phe‐trans‐4‐OH‐l‐Pro), cyclo(l‐Phe‐l‐Pro), and cyclo(l‐Trp‐l‐Pro).
Bibliography:	ArticleID:CBDV201500514 ark:/67375/WNG-MWVW42ZN-D Secretaria da Comissão Interministerial dos Recursos do Mar (SECIRM) Program PROARQUIPELAGO Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) istex:EE75A4D2A1BC48C005215B2FDAA5A45C5E2F4A75 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1612-1872 1612-1880
DOI:	10.1002/cbdv.201500514