Search Results - "Coffman, Jonathan L"

Weak partitioning chromatography for anion exchange purification of monoclonal antibodies by D. Kelley, Brian, Tobler, Scott A., Brown, Paul, Coffman, Jonathan L., Godavarti, Ranga, Iskra, Timothy, Switzer, Mary, Vunnum, Suresh

“…Weak partitioning chromatography (WPC) is an isocratic chromatographic protein separation method performed under mobile phase conditions where a significant…”
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Continuous precipitation for monoclonal antibody capture using countercurrent washing by microfiltration by Li, Zhao, Gu, Qin, Coffman, Jonathan L., Przybycien, Todd, Zydney, Andrew L.

“…There is renewed interest in the possibility of using precipitation for initial capture of high‐value therapeutic proteins as part of an integrated continuous…”
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Evaluating end‐to‐end continuous antibody manufacture with column‐free capture alternatives from economic, environmental, and robustness perspectives by Neves, Catarina P. G., Coffman, Jonathan L., Farid, Suzanne S.

“…Process intensification efforts have renewed interest in the potential of end‐to‐end continuous manufacture with column‐free capture alternatives. This article…”
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High throughput solubility and redissolution screening for antibody purification via combined PEG and zinc chloride precipitation by Gu, Qin, Li, Zhao, Coffman, Jonathan L., Przybycien, Todd M., Zydney, Andrew L.

“…As upstream product titers increase, the downstream chromatographic capture step has become a significant “downstream bottleneck.” Precipitation becomes more…”
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High-throughput screening of chromatographic separations: I. Method development and column modeling by Coffman, Jonathan L, Kramarczyk, Jack F, Kelley, Brian D

“…The development of purification processes for protein biopharmaceuticals is challenging due to compressed development timelines, long experimental times, and…”
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Enhanced filtration performance using feed‐and‐bleed configuration for purification of antibody precipitates by Li, Zhao, Chen, Ting‐Hsi, Andini, Erha, Coffman, Jonathan L, Przybycien, Todd, Zydney, Andrew L.

“…Precipitation can be used for the initial purification of monoclonal antibodies (mAbs), with the soluble host cell proteins removed in the permeate by…”
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High-throughput screening of chromatographic separations: II. Hydrophobic interaction by Kramarczyk, Jack F, Kelley, Brian D, Coffman, Jonathan L

“…A high-throughput screen (HTS) was developed to evaluate the selectivity of various hydrophobic interaction chromatography (HIC) resins for separating a mAb…”
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Feasibility of spectral pH measurement during the low‐pH virus inactivation step of continuous therapeutic antibody production by Goby, Jeffrey D., Furuya, Kenji, Zimmermann, Eike, Beller, Justin A., Schmitt, John M., Cortese, Margot, Breit, Jeffrey F., Coffman, Jonathan L.

“…Acidic virus inactivation is commonly used during production of biotherapeutic products to provide virus safety in case of undetected virus contamination…”
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The effect of protein a cycle number on the performance and lifetime of an anion exchange polishing step by Iskra, Timothy, Bolton, Glen R., Coffman, Jonathan L., Godavarti, Ranga

“…Most mAb platform purification processes consist of an affinity capture step followed by one or two polishing steps. An understanding of the performance…”
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Protein Diffusion in Porous Chromatographic Media Studied by Proton and Fluorine PFG-NMR by Coffman, Jonathan L, Lightfoot, Edwin N, Root, Thatcher W

“…Diffusivities of several proteins or protein variants in nonadsorbing rigid-polymer and bonded-phase-silica chromatographic media were determined using…”
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Addressing the Challenges in Downstream Processing Today and Tomorrow by Bolton, Glen R, Violand, Bernard N, Wright, Richard S, Sun, Shujun, Sunasara, Khurram M, Watson, Kathleen, Coffman, Jonathan L, Gallo, Christopher, Godavarti, Ranga

“…In recent years, most pharmaceutical companies have focused on the development of monoclonal antibodies (mAbs). Increasing upstream titers and shrinking…”
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High-resolution chromatography of proteins in short columns and adsorptive membranes by Coffman, J L, Roper, D K, Lightfoot, E N

“…Short chromatographic columns prepared from stacks of microporous adsorptive membranes are promising for preparative-scale fractionation of even rather closely…”
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Addressing the challenges in downstream processing today and tomorrow: newer classes of biotherapeutics will require innovations in processing technology by Bolton, Glen R, Violand, Bernard N, Wright, Richard S, Sun, Shujun, Sunasara, Khurram M, Watson, Kathleen, Coffman, Jonathan L, Gallo, Christopher, Godavarti, Ranga

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