Environmental assessment of sewage contamination in the surroundings of a marine outfall combining human mastadenovirus and fecal indicator bacteria

This study assessed the microbiological contamination of the marine area of a metropolitan region, where a marine outfall is used as a sanitary solution for domestic sewage. For human mastadenovirus (HAdV) quantification 134 water samples were concentrated by skimmed milk flocculation method and ana...

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Published in:Marine pollution bulletin Vol. 193; p. 115110
Main Authors: Pedrosa de Macena, Lorena da Graça, Castiglia Feitosa, Renato, Couto da Silva, Jansen, Ferreira, Fernando César, Maranhão, Adriana Gonçalves, Brandão, Marcelo Luiz Lima, Caldeira, Nathalia Gonçalves Santos, Couto, Jessica Soldani, Coelho de Azevedo, Mariana Gonçalves, Barbosa de Paula, Bruna, Miagostovich, Marize Pereira
Format: Journal Article
Language:English
Published: England Elsevier Ltd 01-08-2023
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Summary:This study assessed the microbiological contamination of the marine area of a metropolitan region, where a marine outfall is used as a sanitary solution for domestic sewage. For human mastadenovirus (HAdV) quantification 134 water samples were concentrated by skimmed milk flocculation method and analyzed with qPCR and PMAxx-qPCR, being the latter to assess the capsid integrity viral. HAdV with intact capsids were detected in 10 % (16/102) of samples classified as suitable for bathing using at least one fecal bacterial indicator. Spatial analysis of the results showed that the drainage channels of the basin that flow into the sea are the main sources of microbiological contamination in the foreshore zone, where intact HAdV reached a concentration of up to 3 log genomic copies/L. HAdV serotypes A12, D, F40 and F41 were characterized. Our results suggest the use of intact HAdV as a complementary parameter to assess the quality of recreational waters. [Display omitted] •Intact HAdV was detected in marine recreational waters using PMAxx-qPCR.•Watershed channels were the main sources of fecal contamination on the beaches.•Intact HAdV was a promising complementary indicator of human fecal contamination.
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ISSN:0025-326X
1879-3363
DOI:10.1016/j.marpolbul.2023.115110