Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and...

Full description

Saved in:

Bibliographic Details
Published in:	Scientific reports Vol. 11; no. 1; p. 16482
Main Authors:	Oliveira, Hellen Braga Martins, Selis, Nathan das Neves, Sampaio, Beatriz Almeida, Júnior, Manoel Neres Santos, de Carvalho, Suzi Pacheco, de Almeida, Jéssica Bomfim, Almeida, Palloma Porto, da Silva, Icaro Bonyek Santos, Oliveira, Caline Novais Teixeira, Brito, Thamara Louisy Santos, da Silva, Letícia de Oliveira, Teixeira, Mariana Morais, Coelho, Hanna Izadora Laís Novaes, Barbosa, Camila Dutra, Andrade, Yasmin Monara Ferreira de Sousa, Bittencourt, Rafaela de Souza, Viana, Jully Chayra Santos, Campos, Guilherme Barreto, Timenetsky, Jorge, Uetanabaro, Ana Paula T., Yatsuda, Regiane, Marques, Lucas Miranda
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 13-08-2021 Nature Publishing Group Nature Portfolio
Subjects:	631/326 631/326/421 Antibiotics Antimicrobial activity Antimicrobial agents Biofilms Citral Drug resistance Humanities and Social Sciences Methicillin Minimum inhibitory concentration Morbidity multidisciplinary Pharmaceutical industry Science Science (multidisciplinary) Staphylococcal enterotoxin G Staphylococcus aureus Staphylococcus infections Virulence Virulence factors
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and modulating virulence genes, with the ultimate goal of finding a strategy for treating infections caused by MRSA strains. Citral showed antimicrobial activity against MRSA isolates with minimum inhibitory concentration (MIC) values between 5 mg/mL (0.5%) and 40 mg/mL (4%), and minimum bactericidal concentration (MBC) values between 10 mg/mL (1%) and 40 mg/mL (4%). The sub-inhibitory dose was 2.5 mg/mL (0.25%). Citral, in an antibiogram, modulated synergistically, antagonistically, or indifferent to the different antibiotics tested. Prior to evaluating the antibiofilm effects of citral, we classified the bacteria according to their biofilm production capacity. Citral showed greater efficacy in the initial stage, and there was a significant reduction in biofilm formation compared to the mature biofilm. qPCR was used to assess the modulation of virulence factor genes, and ica A underexpression was observed in isolates 20 and 48. For ica D, seg , and sei , an increase was observed in the expression of ATCC 33,591. No significant differences were found for eta and etb . Citral could be used as a supplement to conventional antibiotics for MRSA infections.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2045-2322 2045-2322
DOI:	10.1038/s41598-021-95971-y