Vesicular ATPase-overexpressing cells determine the distribution of malaria parasite oocysts on the midguts of mosquitoes

In Plasmodium -infected mosquitoes, oocysts are preferentially located at the posterior half of the posterior midgut. Because mosquitoes rest vertically after feeding, the effect of gravity on the ingested blood has been proposed as the cause of such a biased distribution. In this paper, we examined...

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 274; no. 18; pp. 12650 - 12655
Main Authors:	Cociancich, S.O, Park, S.S, Fidock, D.A, Shahabuddin, M
Format:	Journal Article
Language:	English
Published:	United States American Society for Biochemistry and Molecular Biology 30-04-1999
Subjects:	Adenosine Triphosphatases - chemistry Adenosine Triphosphatases - genetics Adenosine Triphosphatases - metabolism adenosinetriphosphatase Aedes - enzymology Aedes - parasitology Aedes aegypti Amino Acid Sequence amino acid sequences Animals Anopheles aegypti Anopheles gambiae Base Sequence complementary DNA Culicidae DNA, Complementary genbank/af092934 intestinal mucosa Intestines - parasitology midgut Molecular Sequence Data nucleotide sequences oocysts Open Reading Frames Plasmodium Plasmodium gallinaceum Plasmodium gallinaceum - growth & development Plasmodium gallinaceum - isolation & purification Sequence Homology, Amino Acid spatial distribution
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Summary:	In Plasmodium -infected mosquitoes, oocysts are preferentially located at the posterior half of the posterior midgut. Because mosquitoes rest vertically after feeding, the effect of gravity on the ingested blood has been proposed as the cause of such a biased distribution. In this paper, we examined the oocyst distribution on the midguts of mosquitoes that were continuously rotated to nullify the effect of gravity and found that the typical pattern of oocyst distribution did not change. Invasion of the midgut epithelium by ookinetes was similarly found to be biased toward the posterior part of the posterior midgut. We examined whether the distribution of oocysts depends on the distribution of vesicular ATPase (V-ATPase)-overexpressing cells that Plasmodium ookinetes preferentially use to cross the midgut epithelium. An antiserum raised against recombinant Aedes aegypti V-ATPase B subunit indicated that the majority of V-ATPase-overexpressing cells in Ae. aegypti and Anopheles gambiae are localized at the posterior part of the posterior midgut. We propose that the typical distribution of oocysts on the mosquito midgut is attributable to the presence and the spatial distribution of the V-ATPase-overexpressing cells in the midgut epithelium.
Bibliography:	http://www.jbc.org/ ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0021-9258 1083-351X
DOI:	10.1074/jbc.274.18.12650