Integrated Biosensor for Rapid and Point-of-Care Sepsis Diagnosis

Sepsis is an often fatal condition that arises when the immune response to an infection causes widespread systemic organ injury. A critical unmet need in combating sepsis is the lack of accurate early biomarkers that produce actionable results in busy clinical settings. Here, we report the developme...

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Bibliographic Details
Published in:	ACS nano Vol. 12; no. 4; pp. 3378 - 3384
Main Authors:	Min, Jouha, Nothing, Maria, Coble, Ben, Zheng, Hui, Park, Jongmin, Im, Hyungsoon, Weber, Georg F, Castro, Cesar M, Swirski, Filip K, Weissleder, Ralph, Lee, Hakho
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 24-04-2018
Subjects:	Biosensing Techniques - instrumentation Enzyme-Linked Immunosorbent Assay Humans Point-of-Care Systems Sepsis - blood Sepsis - diagnosis electrochemical sensing IL-3 point-of-care sepsis organ failure
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Summary:	Sepsis is an often fatal condition that arises when the immune response to an infection causes widespread systemic organ injury. A critical unmet need in combating sepsis is the lack of accurate early biomarkers that produce actionable results in busy clinical settings. Here, we report the development of a point-of-care platform for rapid sepsis detection. Termed IBS (integrated biosensor for sepsis), our approach leverages (i) the pathophysiological role of cytokine interleukin-3 (IL-3) in early sepsis and (ii) a hybrid magneto-electrochemical sensor for IL-3 detection. The developed platform produces test results within 1 h from native blood samples and detects IL-3 at a sensitivity of <10 pg/mL; this performance is >5-times faster and >10-times more sensitive than conventional enzyme-linked immunoadsorbent assays, the current gold standard. Using clinical samples, we show that elevated plasma IL-3 levels are associated with high organ failure rate and thus greater risk of mortality, confirming the potential of IL-3 as a sepsis diagnostic biomarker. With further system development (e.g., full automation, data security measures) and rigorous validation studies, the compact and fast IBS could be a practical clinical tool for timely diagnosis and proactive treatment of sepsis.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1936-0851 1936-086X
DOI:	10.1021/acsnano.7b08965