Radiation inactivation of Paenibacillus larvae and sterilization of American Foul Brood (AFB) infected hives using Co-60 gamma rays

The effectiveness of gamma radiation in inactivating the Philippine isolate of Paenibacillus larvae was investigated. Spores of P. larvae were irradiated at incremental doses (0.1, 0.2, 0.4, 0.8 and 1.6 kGy) of gamma radiation emitted by a ⁶⁰Co source. Surviving spores were counted and used to estim...

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Bibliographic Details
Published in:	Applied radiation and isotopes Vol. 69; no. 10; pp. 1374 - 1379
Main Authors:	De Guzman, Zenaida M, Cervancia, Cleofas R, Dimasuay, Kris Genelyn B, Tolentino, Mitos M, Abrera, Gina B, Cobar, Ma Lucia C, Fajardo, Jr, Alejandro C, Sabino, Noel G, Manila-Fajardo, Analinda C, Feliciano, Chitho P
Format:	Journal Article
Language:	English
Published:	England 01-10-2011
Subjects:	Animals Bees Cobalt Radioisotopes - therapeutic use Dose-Response Relationship, Radiation Gamma Rays Gram-Positive Bacterial Infections - radiotherapy Gram-Positive Bacterial Infections - veterinary Paenibacillus - radiation effects Paenibacillus larvae Spores, Bacterial - radiation effects
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Summary:	The effectiveness of gamma radiation in inactivating the Philippine isolate of Paenibacillus larvae was investigated. Spores of P. larvae were irradiated at incremental doses (0.1, 0.2, 0.4, 0.8 and 1.6 kGy) of gamma radiation emitted by a ⁶⁰Co source. Surviving spores were counted and used to estimate the decimal reduction (D₁₀) value. A dose of 0.2 kGy was sufficient to inactivate 90% of the total recoverable spores from an initial count of 10⁵- 9 × 10³ spores per glass plate. The sterilizing effect of high doses of gamma radiation on the spores of P. larvae in infected hives was determined. In this study, a minimum dose (D(min)) of 15 kGy was tested. Beehives with sub-clinical infections of AFB were irradiated and examined for sterility. All the materials were found to be free of P. larvae indicating its susceptibility to γ-rays. After irradiation, there were no visible changes in the physical appearance of the hives' body, wax and frames. Thus, a dose of 15 kGy is effective enough for sterilization of AFB-infected materials.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0969-8043 1872-9800
DOI:	10.1016/j.apradiso.2011.05.032