Response of bacterial biofilms in recirculating aquaculture systems to various sanitizers

Response of bacterial biofilms in recirculating aquaculture systems to various sanitizers

Pathogenic microorganisms may be incorporated into biofilms found in aquaculture systems, causing recurring exposure to potential disease agents. Aerobic plate counts, the presence of Enterobacteriaceae, and the presence of Escherichia coli, modified to express a green fluorescent protein (GFP E. co...

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Bibliographic Details
Published in:Journal of applied aquaculture Vol. 20; no. 2; pp. 79 - 92
Main Authors: King, Robin K, Flick, George J. Jr, Smith, Stephen A, Pierson, Merle D, Boardman, Gregory D, Coale, Charles W. Jr
Format: Journal Article
Language:English
Published: Taylor & Francis Group 30-05-2008
Subjects:
ammonium compounds
Aquaculture
bacteria
biofilm
biofilms
Brackish
cleaning agents
disease control
E. coli
Enterobacteriaceae
Escherichia coli
fish
green fluorescent protein
Oreochromis niloticus
pathogen
pathogens
peracetic acid
plate count
recirculating aquaculture systems
sanitizers
sodium hypochlorite
water
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Summary:Pathogenic microorganisms may be incorporated into biofilms found in aquaculture systems, causing recurring exposure to potential disease agents. Aerobic plate counts, the presence of Enterobacteriaceae, and the presence of Escherichia coli, modified to express a green fluorescent protein (GFP E. coli), was used to evaluate the effectiveness of various sanitizers in decreasing bacterial incorporation into newly generated biofilms in recirculating aquaculture systems. Disks of Buna-N rubber, polyvinyl chloride (PVC), chlorinated PVC, glass, fiberglass, and stainless steel were placed in aquariums stocked with Nile tilapia, Oreochromis niloticus. The effectiveness of water, an alkaline cleanser, sodium hypochlorite, a quaternary ammonium compound, or peracetic acid as a sanitizer was evaluated on each substrate by enumerating total plate counts, GFP E. coli, and Enterobacteriaceae. Sodium hypochlorite and peracetic acid were the most effective sanitizers, with an overall percentage reduction of GFP E. coli of approximately 2 logs 10 . The quaternary ammonium compound was moderately effective, 1 log 10 , against the target organisms. Water demonstrated a 2 log 10 reduction of the total plate count, suggesting that some mechanical cleaning was achieved. The type of material used as substrate for the biofilm had no significant (P > 0.05) effect on the effectiveness of the sanitizers.
Bibliography:http://hdl.handle.net/10113/20299
ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1045-4438
1545-0805
DOI:10.1080/10454430802191766