Search Results - "Clowsley, Alexander H"

Repeat DNA-PAINT suppresses background and non-specific signals in optical nanoscopy by Clowsley, Alexander H., Kaufhold, William T., Lutz, Tobias, Meletiou, Anna, Di Michele, Lorenzo, Soeller, Christian

“…DNA-PAINT is a versatile optical super-resolution technique relying on the transient binding of fluorescent DNA ‘imagers’ to target epitopes. Its performance…”
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True Molecular Scale Visualization of Variable Clustering Properties of Ryanodine Receptors by Jayasinghe, Izzy, Clowsley, Alexander H., Lin, Ruisheng, Lutz, Tobias, Harrison, Carl, Green, Ellen, Baddeley, David, Di Michele, Lorenzo, Soeller, Christian

“…Signaling nanodomains rely on spatial organization of proteins to allow controlled intracellular signaling. Examples include calcium release sites of…”
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Shining New Light on the Structural Determinants of Cardiac Couplon Function: Insights From Ten Years of Nanoscale Microscopy by Jayasinghe, Izzy, Clowsley, Alexander H, de Langen, Oscar, Sali, Sonali S, Crossman, David J, Soeller, Christian

“…Remodelling of the membranes and protein clustering patterns during the pathogenesis of cardiomyopathies has renewed the interest in spatial visualisation of…”
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3D super-resolution microscopy performance and quantitative analysis assessment using DNA-PAINT and DNA origami test samples by Lin, Ruisheng, Clowsley, Alexander H., Lutz, Tobias, Baddeley, David, Soeller, Christian

“…•Routine testing and calibration are essential for high quality super-resolution imaging.•We present a number of time and cost effective DNA-PAINT based test…”
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Detecting Nanoscale Distribution of Protein Pairs by Proximity-Dependent Super-resolution Microscopy by Clowsley, Alexander H, Kaufhold, William T, Lutz, Tobias, Meletiou, Anna, Di Michele, Lorenzo, Soeller, Christian

“…Interactions between biomolecules such as proteins underlie most cellular processes. It is crucial to visualize these molecular-interaction complexes directly…”
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Reduced Non-Specific Binding of Super-Resolution DNA-PAINT Markers by Shielded DNA-PAINT Labeling Protocols by Lučinskaitė, Evelina, Bokhobza, Alexandre F E, Stannard, Andrew, Meletiou, Anna, Estell, Chris, West, Steven, Michele, Lorenzo Di, Soeller, Christian, Clowsley, Alexander H

“…The DNA-based single molecule super-resolution imaging approach, DNA-PAINT, can achieve nanometer resolution of single targets. However, the approach can…”
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Algorithmic corrections for localization microscopy with sCMOS cameras - characterisation of a computationally efficient localization approach by Lin, Ruisheng, Clowsley, Alexander H, Jayasinghe, Isuru D, Baddeley, David, Soeller, Christian

“…Modern sCMOS cameras are attractive for single molecule localization microscopy (SMLM) due to their high speed but suffer from pixel non-uniformities that can…”
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Versatile multiplexed super-resolution imaging of nanostructures by Quencher-Exchange-PAINT by Lutz, Tobias, Clowsley, Alexander H., Lin, Ruisheng, Pagliara, Stefano, Di Michele, Lorenzo, Soeller, Christian

“…The optical super-resolution technique DNA-PAINT (Point Accumulation Imaging in Nanoscale Topography) provides a flexible way to achieve imaging of nanoscale…”
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Three-Dimensional and Chemical Mapping of Intracellular Signaling Nanodomains in Health and Disease with Enhanced Expansion Microscopy by Sheard, Thomas M. D, Hurley, Miriam E, Colyer, John, White, Ed, Norman, Ruth, Pervolaraki, Eleftheria, Narayanasamy, Kaarjel K, Hou, Yufeng, Kirton, Hannah M, Yang, Zhaokang, Hunter, Liam, Shim, Jung-uk, Clowsley, Alexander H, Smith, Andrew J, Baddeley, David, Soeller, Christian, Colman, Michael A, Jayasinghe, Izzy

“…Nanodomains are intracellular foci which transduce signals between major cellular compartments. One of the most ubiquitous signal transducers, the ryanodine…”
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Revealing t-tubules in striated muscle with new optical super-resolution microscopy techniques by Jayasinghe, Isuru D., Clowsley, Alexander H., Munro, Michelle, Hou, Yufeng, Crossman, David J., Soeller, Christian

“…The t-tubular system plays a central role in the synchronisation of calcium signalling and excitation-contraction coupling in most striated muscle cells. Light…”
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Revealing T-Tubules in Striated Muscle with New Optical Super-Resolution Microscopy Techniquess by Jayasinghe, Isuru D., Clowsley, Alexander H., Munro, Michelle, Hou, Yufeng, Crossman, David J., Soeller, Christian

“…The t-tubular system plays a central role in the synchronisation of calcium signalling and excitation-contraction coupling in most striated muscle cells. Light…”
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Versatile Multiplexed Super-Resolution Imaging of Nanostructures by Quencher-Exchange-Paint by Lutz, Tobias, Clowsley, Alexander H., Lin, Ruisheng, Pagliara, Stefano, di Michele, Lorenzo, Soeller, Christian

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Revealing t-tubules in striated muscle with new optical super-resolution microscopy techniques by Jayasinghe, Isuru D., Clowsley, Alexander H., Munro, Michelle, Hou, Yufeng, Crossman, David J., Soeller, Christian

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