CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation

CD1d is a nonpolymorphic MHC class I-like molecule which presents nonpeptide ligands, e.g. glycolipids, to NKT cells. These cells are known to have multiple effects on innate and adaptive immune responses and on the development of pathological conditions. In order to analyze CD1d expression and func...

Full description

Saved in:

Bibliographic Details
Published in:	PloS one Vol. 5; no. 9; p. e13089
Main Authors:	Monzon-Casanova, Elisa, Steiniger, Birte, Schweigle, Stefanie, Clemen, Holger, Zdzieblo, Daniela, Starick, Lisa, Müller, Ingrid, Wang, Chyung-Ru, Rhost, Sara, Cardell, Susanna, Pyz, Elwira, Herrmann, Thomas
Format:	Journal Article
Language:	English
Published:	United States Public Library of Science 30-09-2010 Public Library of Science (PLoS)
Subjects:	Adaptive immunity Animal tissues Animals Antibodies, Monoclonal - analysis Antibodies, Monoclonal - immunology Antigen presentation Antigenic determinants Antigens, CD1 - genetics Antigens, CD1 - immunology Apoptosis Autoimmunity B cells Beta cells CD1d antigen CD4 antigen Cell activation Cell Line Cell surface Cytometry Dendritic cells Epitopes Female Flora Flow cytometry Galactosylceramide Gene Expression Genes Glycolipids Immune response Immunoglobulins Immunology Immunology/Antigen Processing and Recognition Immunology/Genetics of the Immune System Immunology/Immune Response Immunology/Innate Immunity Intestinal microflora Lipids Lymphocyte Activation Lymphocytes Lymphocytes T Major histocompatibility complex Male Membrane lipids Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Knockout Microbiology in the medical area Microbiota (Symbiotic organisms) Mikrobiologi inom det medicinska området Monoclonal antibodies Natural killer cells Natural Killer T-Cells - chemistry Natural Killer T-Cells - immunology Pancreas Pancreas, Exocrine Paneth cells Paneth Cells - chemistry Paneth Cells - immunology Plant lipids Proteins Rats Rats, Inbred Lew Recognition Rodents T cell receptors T cells Virology United States > US Germany California
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	CD1d is a nonpolymorphic MHC class I-like molecule which presents nonpeptide ligands, e.g. glycolipids, to NKT cells. These cells are known to have multiple effects on innate and adaptive immune responses and on the development of pathological conditions. In order to analyze CD1d expression and function in the rat, the first rat CD1d-specific monoclonal antibodies (mAbs) were generated. Two mAbs, WTH-1 and WTH-2, were generated which bound equally well to cell surface-expressed rat and mouse CD1d. Their non-overlapping epitopes were mapped to the CD1d heavy chain. Flow cytometry and immunohistological analyses revealed a nearly identical degree and pattern of CD1d expression for hematopoieitic cells of both species. Notable is also the detection of CD1d protein in mouse and rat Paneth cells as well as the extremely high CD1d expression in acinar exocrine cells of the rat pancreas and the expression of CD4 on rat marginal zone B cells. Both mAbs blocked α-galactosylceramide recognition by primary rat and mouse NKT cells. Interestingly, the two mAbs differed in their impact on the activation of various autoreactive T cell hybridomas, including the XV19.2 hybridoma whose activation was enhanced by the WTH-1 mAb. The two novel monoclonal antibodies described in this study, allowed the analysis of CD1d expression and CD1d-restricted T cell responses in the rat for the first time. Moreover, they provided new insights into mechanisms of CD1d-restricted antigen recognition. While CD1d expression by hematopoietic cells of mice and rats was extremely similar, CD1d protein was detected at not yet described sites of non-lymphatic tissues such as the rat exocrine pancreas and Paneth cells. The latter is of special relevance given the recently reported defects of Paneth cells in CD1d(-/-) mice, which resulted in an altered composition of the gut flora.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Current address: E.P. Institute for Cell Biology, Department of Immunology, Tübingen, Germany Conceived and designed the experiments: EMC BS EP TH. Performed the experiments: EMC BS SS HC DZ LS IM SR EP. Analyzed the data: EMC BS SS SR SC EP TH. Contributed reagents/materials/analysis tools: CRW SC. Wrote the paper: EMC BS TH.
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0013089