Development of 17 microsatellite markers in the federally endangered species Liatris helleri (Asteraceae)

Development of 17 microsatellite markers in the federally endangered species Liatris helleri (Asteraceae)

Premise Microsatellite markers were developed in the federally endangered species Liatris helleri (Asteraceae) to evaluate species boundaries with closely related congeners within the genus. Methods and Results Using Illumina data, 17 primer pairs were developed in populations of L. helleri. The pri...

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Bibliographic Details
Published in:Applications in plant sciences Vol. 7; no. 7; pp. e01250 - n/a
Main Authors: Clark, Logan C., Gaglianese‐Woody, Morgan R., Estep, Matt C.
Format: Journal Article
Language:English
Published: United States John Wiley & Sons, Inc 01-07-2019
John Wiley and Sons Inc
Wiley
Subjects:
Asteraceae
Congeners
Deoxyribonucleic acid
DNA
Endangered & extinct species
Endangered species
Genetic analysis
Genetic diversity
Genetic markers
Genomics
Liatris helleri
Microsatellites
perennial herb
Phenology
Plant sciences
Population genetics
Primer Note
Primer Notes
Primers
Southern Appalachians
species boundaries
Thermal cycling
Wildlife conservation
United States > US
Georgia
California
endangered species
species boundaries
Liatris helleri
perennial herb
Asteraceae
Southern Appalachians
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Summary:Premise Microsatellite markers were developed in the federally endangered species Liatris helleri (Asteraceae) to evaluate species boundaries with closely related congeners within the genus. Methods and Results Using Illumina data, 17 primer pairs were developed in populations of L. helleri. The primers amplified motifs from tri‐ to hexanucleotide repeats with one to 17 alleles per locus. Primers were also tested for cross‐amplification in L. aspera, L. microcephala, and L. pycnostachya. Conclusions The developed primers for L. helleri serve as a novel genetic tool for future investigations in this genus, allowing for more explicit species delineation as well as population genetic analyses.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:2168-0450
2168-0450
DOI:10.1002/aps3.1250