Search Results - "Ciesla, Joanna"

Phosphorylation of basic amino acid residues in proteins: important but easily missed by Cieśla, Joanna, Frączyk, Tomasz, Rode, Wojciech

“…Reversible phosphorylation is the most widespread posttranslational protein modification, playing regulatory role in almost every aspect of cell life. The…”
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A synergistic effect of phosphate, pH and Phe159 substitution on the formycin A association to the E. coli purine nucleoside phosphorylase by Prokopowicz, Małgorzata, Cieśla, Joanna, Kierdaszuk, Borys

“…A steady-state absorption and emission spectroscopy was used to create a comprehensive work and to study the interaction of the wild type Escherichia coli…”
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Human dihydrofolate reductase is a substrate of protein kinase CK2α by Skierka, Katarzyna, Wilamowski, Paweł, Wielechowska, Monika, Cysewski, Dominik, Senkara, Elżbieta, Wińska, Patrycja, Bretner, Maria, Cieśla, Joanna

“…Dihydrofolate reductase (DHFR) is a prominent molecular target in antitumor, antibacterial, antiprotozoan, and immunosuppressive chemotherapies, and CK2…”
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Development of lactic acid production from coffee grounds hydrolysate by fermentation with Lacticaseibacillus rhamnosus by Wysocki, Łukasz, Adamczuk, Patrycja, Bardadyn, Paula, Gabor, Anna, Jelonek, Karolina, Kudelska, Monika, Kukuć, Maksymilian, Piasek, Adrianna, Pietras, Marta, Słomka, Monika, Trojan, Zoja, Tybulczuk, Wiktoria, Sobiepanek, Anna, Żylińska-Urban, Joanna, Cieśla, Joanna

“…Abstract   Spent coffee grounds (SCG) are commercial waste that are still rich in numerous valuable ingredients and can be further processed into useful…”
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Quartz crystal microbalance with dissipation and microscale thermophoresis as tools for investigation of protein complex formation between thymidylate synthesis cycle enzymes by Antosiewicz, Anna, Senkara, Elżbieta, Cieśla, Joanna

“…Thymidylate synthase (TS) and dihydrofolate reductase (DHFR) play essential role in DNA synthesis, repair and cell division by catalyzing two subsequent…”
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Effect of Simultaneous Inhibition of Protein Kinase CK2 and Thymidylate Synthase in Leukemia and Breast Cancer Cells by Wińska, Patrycja, Skierka, Katarzyna, Łukowska-Chojnacka, Edyta, Koronkiewicz, Mirosława, Cieśla, Joanna, Bretner, Maria

“…Protein kinase CK2 was recently identified as a promising therapeutic target for combination therapy. Our study aims to investigate the anticancer effect of a…”
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Simultaneous Inhibition of Protein Kinase CK2 and Dihydrofolate Reductase Results in Synergistic Effect on Acute Lymphoblastic Leukemia Cells by Wińska, Patrycja, Widło, Łukasz, Skierka, Katarzyna, Krzyśko, Alicja, Koronkiewicz, Mirosława, Cieśla, Jarosław M, Cieśla, Joanna, Bretner, Maria

“…Recently, we demonstrated the ability of inhibitors of protein kinase 2 (casein kinase II; CK2) to enhance the efficacy of 5-fluorouracil, a thymidylate…”
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Towards understanding the E. coli PNP binding mechanism and FRET absence between E. coli PNP and formycin A by Prokopowicz, Małgorzata, Greń, Bartosz, Cieśla, Joanna, Kierdaszuk, Borys

“…The aim of this study is threefold: (1) augmentation of the knowledge of the E. coli PNP binding mechanism; (2) explanation of the previously observed ‘lack of…”
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Human dihydrofolate reductase and thymidylate synthase form a complex in vitro and co-localize in normal and cancer cells by Antosiewicz, Anna, Jarmuła, Adam, Przybylska, Dorota, Mosieniak, Grażyna, Szczepanowska, Joanna, Kowalkowska, Anna, Rode, Wojciech, Cieśla, Joanna

“…Enzymes involved in thymidylate biosynthesis, thymidylate synthase (TS), and dihydrofolate reductase (DHFR) are well-known targets in cancer chemotherapy. In…”
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Mouse thymidylate synthase does not show the inactive conformation, observed for the human enzyme by Dowierciał, Anna, Jarmuła, Adam, Wilk, Piotr, Rypniewski, Wojciech, Kowalska, Monika, Frączyk, Tomasz, Cieśla, Joanna, Rode, Wojciech

“…Crystal structures of mouse thymidylate synthase (mTS) in complexes with (1) sulfate anion, (2) 2′-deoxyuridine 5′-monophosphate (dUMP) and (3) 5-fluoro-dUMP…”
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Crystal structures of nematode (parasitic T. spiralis and free living C. elegans), compared to mammalian, thymidylate synthases (TS). Molecular docking and molecular dynamics simulations in search for nematode-specific inhibitors of TS by Jarmuła, Adam, Wilk, Piotr, Maj, Piotr, Ludwiczak, Jan, Dowierciał, Anna, Banaszak, Katarzyna, Rypniewski, Wojciech, Cieśla, Joanna, Dąbrowska, Magdalena, Frączyk, Tomasz, Bronowska, Agnieszka K., Jakowiecki, Jakub, Filipek, Sławomir, Rode, Wojciech

“…[Display omitted] •Trichinellosis is a serious disease in both developing and developed countries.•Crystal structures of ligand-bound and -free nematode…”
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Crystal structure of phosphoramide-phosphorylated thymidylate synthase reveals pSer127, reflecting probably pHis to pSer phosphotransfer by Wilk, Piotr, Jarmuła, Adam, Ruman, Tomasz, Banaszak, Katarzyna, Rypniewski, Wojciech, Cieśla, Joanna, Dowierciał, Anna, Rode, Wojciech

“…•31P NMR found thymidylate synthase phosphorylated only on histidine residue(s).•Crystal structure of the enzyme-dUMP complex showed a single phosphoserine…”
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Thiophosphorylation of free amino acids and enzyme protein by thiophosphoramidate ions by Ruman, Tomasz, Długopolska, Karolina, Jurkiewicz, Agata, Rut, Dagmara, Frączyk, Tomasz, Cieśla, Joanna, Leś, Andrzej, Szewczuk, Zbigniew, Rode, Wojciech

“…In search of an activity-preserving protein thiophosphorylation method, with thymidylate synthase recombinant protein used as a substrate, potassium…”
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Phosphorylation of Thymidylate Synthase and Dihydrofolate Reductase in Cancer Cells and the Effect of CK2α Silencing by Wińska, Patrycja, Sobiepanek, Anna, Pawlak, Katarzyna, Staniszewska, Monika, Cieśla, Joanna

“…Our previous research suggests an important regulatory role of CK2-mediated phosphorylation of enzymes involved in the thymidylate biosynthesis cycle, i.e.,…”
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Phosphorylation of thymidylate synthase from various sources by human protein kinase CK2 and its catalytic subunits by Frączyk, Tomasz, Kubiński, Konrad, Masłyk, Maciej, Cieśla, Joanna, Hellman, Ulf, Shugar, David, Rode, Wojciech

“…Thymidylate synthase (TS) was a substrate for human CK2 catalytic subunits. MALDI-TOF MS analysis of sulfonated protein digest led to identification of…”
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Immunofluorescent localization of thymidylate synthase in the development of Trichinella spiralis and Caenorhabditis elegans by Gołos, Barbara, Dąbrowska, Magdalena, Wałajtys-Rode, Elżbieta, Zieliński, Zbigniew, Wińska, Patrycja, Cieśla, Joanna, Jagielska, Elżbieta, Moczoń, Tadeusz, Rode, Wojciech

“…[Display omitted] ► Non-developing nematode larvae are rich in thymidylate synthase, undetectable in quiescent cells. ► T. spiralis muscle larva nerve ring,…”
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Segmental motions of rat thymidylate synthase leading to half-the-sites behavior by Świniarska, Monika, Leś, Andrzej, Rode, Wojciech, Cieśla, Joanna, Millán-Pacheco, César, Blake, Iván Ortega, Pastor, Nina

“…Thymidylate synthase (TS) is a homodimeric enzyme with two equivalent active sites composed of residues from both subunits. Despite the structural symmetry of…”
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Screening of Antioxidative and Antiproliferative Activities of Crude Polysaccharides Extracted from Six Different Plants by Adewale, Omowumi Oyeronke, Wińska, Patrycja, Krawczyk, Hanna, Grzechnik, Eryk, Cieśla, Joanna

“…Plant polysaccharides have gained interest in medical research for their ability to suppress various diseases, including cancer. However, information on some…”
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Single tryptophan Y160W mutant of homooligomeric E. coli purine nucleoside phosphorylase implies that dimers forming the hexamer are functionally not equivalent by Narczyk, Marta, Mioduszewski, Łukasz, Oksiejuk, Aleksandra, Winiewska-Szajewska, Maria, Wielgus-Kutrowska, Beata, Gojdź, Adrian, Cieśla, Joanna, Bzowska, Agnieszka

“…E. coli purine nucleoside phosphorylase is a homohexamer, which structure, in the apo form, can be described as a trimer of dimers. Earlier studies suggested…”
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Interactions of 2′-fluoro-substituted dUMP analogues with thymidylate synthase by Ziemkowski, Przemysław, Felczak, Krzysztof, Poznański, Jarosław, Kulikowski, Tadeusz, Zieliński, Zbigniew, Cieśla, Joanna, Rode, Wojciech

“…A series of 2′-fluoro-substituted dUMP/FdUMP analogues were synthesized, their interaction with human recombinant thymidylate synthase investigated, and…”
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