Search Results - "Church, George"

Large-scale de novo DNA synthesis: technologies and applications by Kosuri, Sriram, Church, George M

“…This Review discusses large-scale de novo DNA synthesis via oligos or arrays, describes gene assembly and error correction and considers applications for…”
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Rapidly evolving homing CRISPR barcodes by Kalhor, Reza, Mali, Prashant, Church, George M

“…Homing guide RNAs that target Cas9 to their own loci generate diverse barcodes that can trace the lineage of cells. We present an approach for engineering…”
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Unified rational protein engineering with sequence-based deep representation learning by Alley, Ethan C., Khimulya, Grigory, Biswas, Surojit, AlQuraishi, Mohammed, Church, George M.

“…Rational protein engineering requires a holistic understanding of protein function. Here, we apply deep learning to unlabeled amino-acid sequences to distill…”
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Next-Generation Digital Information Storage in DNA by Church, George M., Gao, Yuan, Kosuri, Sriram

“…Digital information is accumulating at an astounding rate, straining our ability to store and archive it. DNA is among the most dense and stable information…”
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A Logic-Gated Nanorobot for Targeted Transport of Molecular Payloads by Douglas, Shawn M., Bachelet, Ido, Church, George M.

“…We describe an autonomous DNA nanorobot capable of transporting molecular payloads to cells, sensing cell surface inputs for conditional, triggered activation,…”
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Comprehensive AAV capsid fitness landscape reveals a viral gene and enables machine-guided design by Ogden, Pierce J, Kelsic, Eric D, Sinai, Sam, Church, George M

“…Adeno-associated virus (AAV) capsids can deliver transformative gene therapies, but our understanding of AAV biology remains incomplete. We generated the…”
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Genetically encoded sensors enable real-time observation of metabolite production by Rogers, Jameson K., Church, George M.

“…Engineering cells to produce valuable metabolic products is hindered by the slow and laborious methods available for evaluating product concentration…”
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DNA sequencing at 40: past, present and future by Shendure, Jay, Balasubramanian, Shankar, Church, George M., Gilbert, Walter, Rogers, Jane, Schloss, Jeffery A., Waterston, Robert H.

“…This review commemorates the 40th anniversary of DNA sequencing, a period in which we have already witnessed multiple technological revolutions and a growth in…”
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Low-N protein engineering with data-efficient deep learning by Biswas, Surojit, Khimulya, Grigory, Alley, Ethan C., Esvelt, Kevin M., Church, George M.

“…Protein engineering has enormous academic and industrial potential. However, it is limited by the lack of experimental assays that are consistent with the…”
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Causes and Effects of N-Terminal Codon Bias in Bacterial Genes by Goodman, Daniel B., Church, George M., Kosuri, Sriram

“…Most amino acids are encoded by multiple codons, and codon choice has strong effects on protein expression. Rare codons are enriched at the N terminus of genes…”
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Developmental barcoding of whole mouse via homing CRISPR by Kalhor, Reza, Kalhor, Kian, Mejia, Leo, Leeper, Kathleen, Graveline, Amanda, Mali, Prashant, Church, George M

“…In vivo barcoding using nuclease-induced mutations is a powerful approach for recording biological information, including developmental lineages; however, its…”
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Terminator-free template-independent enzymatic DNA synthesis for digital information storage by Lee, Henry H., Kalhor, Reza, Goela, Naveen, Bolot, Jean, Church, George M.

“…DNA is an emerging medium for digital data and its adoption can be accelerated by synthesis processes specialized for storage applications. Here, we describe a…”
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The Brain Activity Map Project and the Challenge of Functional Connectomics by Alivisatos, A. Paul, Chun, Miyoung, Church, George M., Greenspan, Ralph J., Roukes, Michael L., Yuste, Rafael

“…The function of neural circuits is an emergent property that arises from the coordinated activity of large numbers of neurons. To capture this, we propose…”
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Deciphering the functions and regulation of brain-enriched A-to-I RNA editing by Li, Jin Billy, Church, George M

“…Brain enriched RNA editing of Adenosine-to-Inosine (A-to-I) increases the amount of information encoded in the genome and diversifies the transcriptome. Here…”
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Syntrophic exchange in synthetic microbial communities by Mee, Michael T., Collins, James J., Church, George M., Wang, Harris H.

“…Metabolic crossfeeding is an important process that can broadly shape microbial communities. However, little is known about specific crossfeeding principles…”
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The role of replicates for error mitigation in next-generation sequencing by Robasky, Kimberly, Lewis, Nathan E., Church, George M.

“…Next-generation sequencing for variant identification is now becoming widespread, although pipelines have not yet been optimized. In this Perspective article,…”
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CRISPR–Cas encoding of a digital movie into the genomes of a population of living bacteria by Shipman, Seth L., Nivala, Jeff, Macklis, Jeffrey D., Church, George M.

“…The authors encode pixel values of a short motion picture into the DNA of a population of Escherichia coli . Movie stored on microbes Among many applications,…”
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Beyond editing to writing large genomes by Chari, Raj, Church, George M.

“…Advances in genome sequencing, editing and synthetic biology have enhanced the feasibility of large-scale genome engineering, termed genome writing. In this…”
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Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems by DiCarlo, James E, Norville, Julie E, Mali, Prashant, Rios, Xavier, Aach, John, Church, George M

“…Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuclease activity…”
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RNA-Guided Human Genome Engineering via Cas9 by Mali, Prashant, Yang, Luhan, Esvelt, Kevin M., Aach, John, Guell, Marc, DiCarlo, James E., Norville, Julie E., Church, George M.

“…Bacteria and archaea have evolved adaptive immune defenses, termed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas)…”
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