Anti-cancer Effect of Cyanidin-3-glucoside from Mulberry via Caspase-3 Cleavage and DNA Fragmentation in vitro and in vivo

Fruits of Morus alba L. (mulberry) have various bioactive compounds such as polyphenols and anthocyanins and used as a herbal medicine. However, the anti-cancer effects and molecular basis have not been elucidated. We isolated the cyanidin-3-glucoside in various cultivar of mulberry by acidified-met...

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Bibliographic Details
Published in:	Anti-cancer agents in medicinal chemistry Vol. 17; no. 11; p. 1519
Main Authors:	Cho, Eugene, Chung, Eun Y, Jang, Hye-Yeon, Hong, On-Yu, Chae, Hee S, Jeong, Young-Ju, Kim, Sun-Young, Kim, Byeong-Soo, Yoo, Dong J, Kim, Jong-Suk, Park, Kwang-Hyun
Format:	Journal Article
Language:	English
Published:	Netherlands 24-11-2017
Subjects:	Animals Anthocyanins - chemistry Anthocyanins - metabolism Anthocyanins - pharmacology Antineoplastic Agents, Phytogenic - chemistry Antineoplastic Agents, Phytogenic - metabolism Antineoplastic Agents, Phytogenic - pharmacology Caspase 3 - metabolism Cell Proliferation - drug effects Cell Survival - drug effects DNA Fragmentation - drug effects Dose-Response Relationship, Drug Drug Screening Assays, Antitumor Female Glucosides - chemistry Glucosides - metabolism Glucosides - pharmacology Humans Mammary Neoplasms, Experimental - drug therapy Mammary Neoplasms, Experimental - metabolism Mammary Neoplasms, Experimental - pathology Mice Mice, Inbred ICR Molecular Structure Morus - chemistry Structure-Activity Relationship Tumor Cells, Cultured Cyanidin-3-glucoside DNA fragmentation nude mice mulberry anti-cancer Morus alba L caspase-3
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Summary:	Fruits of Morus alba L. (mulberry) have various bioactive compounds such as polyphenols and anthocyanins and used as a herbal medicine. However, the anti-cancer effects and molecular basis have not been elucidated. We isolated the cyanidin-3-glucoside in various cultivar of mulberry by acidified-methanol extraction methods. This molecule were compared mass spectroscopic properties by LC-MS/MS and analyzed by 1H and 13C NMR. We examined the anti-cancer effect with molecular mechanisms of the cyanidin-3-glucoside on MDA-MB-453 human breast cancer cells and xenograft animal model. The treatment with the mulberry cyanidin-3-glucoside decreased cell viability in a dose-dependent manner with alteration of apoptotic protein contents, and DNA fragmentation, suggesting that cells undergo apoptosis. Supporting the observations, Treatment with the cyanidin-3-glucoside showed active apoptosis by caspase-3 cleavage and DNA fragmentation through Bcl-2 and Bax pathway. Indeed, cyanidin-3-glucoside inhibits tumor growth in MDA-MB-453 cells-inoculated nude mice. Tumor growth of xenograft nude mouse was significantly reduced compared to the control group by the cyanidin-3-glucoside. The data demonstrate that cyanidin-3-glucoside isolated from mulberry induced apoptosis in breast cancer (MDA-MB-453) cells, and therefore, has a potential as an anti-cancer agent. These results show that mulberry cyanidin-3-glucoside inhibit the proliferation and growth in vitro and in vivo model and, indicating the inhibition of tumor progression.
ISSN:	1875-5992
DOI:	10.2174/1871520617666170327152026