ANTIOXIDANT ACTIVITY AND PHENOLIC CONTENT OF DIFFERENT PARTS OF ORTHOSIPHON STAMINEUS GROWN UNDER DIFFERENT LIGHT INTENSITIES

ANTIOXIDANT ACTIVITY AND PHENOLIC CONTENT OF DIFFERENT PARTS OF ORTHOSIPHON STAMINEUS GROWN UNDER DIFFERENT LIGHT INTENSITIES

Orthosiphon stamineus is an important medicinal plant in South-East Asia. Although already widely cultivated, the influence of growing conditions, especially light intensity, on the antioxidant property of O. stamineus is still unknown. Thus, the objective of this study was to determine the antioxid...

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Bibliographic Details
Published in:Journal of tropical forest science Vol. 24; no. 2; pp. 173 - 177
Main Authors: Farhan, M, Razak, S Abdul, Pin, KY, Chuah, AL
Format: Journal Article
Language:English
Published: Kuala Lumpur Forest Research Institute Malaysia 01-04-2012
Subjects:
Antioxidants
Leaves
Luminous intensity
Medicinal plants
Plant roots
Plants
Room temperature
Seedlings
Stems
Tropical forests
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Summary:Orthosiphon stamineus is an important medicinal plant in South-East Asia. Although already widely cultivated, the influence of growing conditions, especially light intensity, on the antioxidant property of O. stamineus is still unknown. Thus, the objective of this study was to determine the antioxidant activity and phenolic content of different plant parts of O. stamineus and to propose the best environment condition for its cultivation. The leaves, stems and roots of O. stamineus treated in open environment were extracted with methanol for 4 hours in a shaking water bath at 40 °C. Total phenolic content (TPC) and antioxidant activity were determined using Folin-Ciocalteu and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods respectively. In the first part of the experiment, only seedlings grown in the open environment were used. TPC was higher in leaves (230 mg gallic acid g-1 dry weight) compared with stems and roots (160 and 140 mg gallic acid g-1 dry weight respectively). However, antioxidant activity was lower in leaves (78.43%) than stems (80.66%) and roots (80.26%). By selecting leaves as the main part to study, O. stamineus grown under shaded treatment (50 and 70% shaded) as well as open environment were harvested to determine their TPC and antioxidant activities. It was observed that TPC in O. stamineus leaves grown in open environment was highest (230 mg gallic acid g-1 dry weight) compared with shade-grown seedlings. Similarly, antioxidant activity was also highest in seedlings grown in open environment (80.02%) compared with in shade (50% = 77.72%, 70% = 74.37%). Orthosiphon stamineus merupakan tumbuhan ubatan yang popular di Asia Tenggara. Walaupun sudah ditanam secara meluas, pengaruh keadaan penanaman, terutama keamatan cahaya, terhadap ciri antioksida O. stamineus masih tidak diketahui. Oleh itu, objektif kajian ini adalah untuk menentukan aktiviti antioksida dan kandungan fenolik pada bahagian tumbuhan yang berbeza dan mencadangkan keadaan persekitaran yang terbaik untuk penanaman O. stamineus. Bahagian berbeza O. stamineus iaitu daun, batang dan akar yang ditanam di persekitaran terbuka diekstrak dengan 100% metanol selama empat jam dalam rendaman air pada 40 °C. Kandungan jumlah fenol (TPC) dan aktiviti antioksida ditentukan masing-masing menggunakan kaedah Folin-Ciocalteu dan 2,2-difenil-1-pikrilhidrazil (DPPH). Dalam bahagian pertama eksperimen, hanya anak benih yang ditanam di persekitaran terbuka digunakan. Keputusan menunjukkan bahawa kandungan TPC paling tinggi dalam daun dengan nilai 230 mg asid galik g-1 berat kering sampel berbanding dengan batang (160 mg asid galik g-1 berat kering sampel) dan akar (140 mg asid galik g-1 berat kering sampel). Bagi ujian aktiviti antioksida, terdapat perbezaan yang signifikan antara daun (78.43%) dengan batang (80.66%) dan akar (80.26%). Justeru, kami memilih daun untuk kajian selanjutnya. Daun O. stamineus yang ditanam di bawah teduhan (50% dan 70% teduhan) serta persekitaran terbuka dituai untuk menentukan TPC dan aktiviti antioksida. Keputusan menunjukkan daun O. stamineus yang tumbuh di persekitaran terbuka mempunyai TPC yang paling tinggi iaitu 230 mg asid galik g-1 berat kering sampel berbanding yang tumbuh dalam teduhan. Kajian turut menunjukkan bahawa aktiviti antioksida paling tinggi bagi anak benih yang tumbuh di persekitaran terbuka (80.02%) berbanding dengan anak benih yang tumbuh dalam teduhan (50% = 77.72%, 70% = 74.37%).
ISSN:0128-1283
2521-9847