Search Results - "Chrast, Lukas"

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  1. 1

    Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway by Dvorak, Pavel, Chrast, Lukas, Nikel, Pablo I, Fedr, Radek, Soucek, Karel, Sedlackova, Miroslava, Chaloupkova, Radka, de Lorenzo, Víctor, Prokop, Zbynek, Damborsky, Jiri

    Published in Microbial cell factories (21-12-2015)
    “…Heterologous expression systems based on promoters inducible with isopropyl-β-D-1-thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21(DE3) and cognate…”
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    Journal Article
  2. 2

    Gram-scale production of recombinant microbial enzymes in shake flasks by Chrast, Lukas, Chaloupkova, Radka, Damborsky, Jiri

    Published in FEMS microbiology letters (01-02-2018)
    “…Heterologous production of recombinant proteins is a cornerstone of microbiological and biochemical research as well as various biotechnological processes…”
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    Journal Article
  3. 3

    Influence of soil γ-irradiation and spiking on sorption of p,p′-DDE and soil organic matter chemistry by Škulcová, Lucia, Scherr, Kerstin E., Chrást, Lukáš, Hofman, Jakub, Bielská, Lucie

    Published in Ecotoxicology and environmental safety (15-07-2018)
    “…The fate of organic chemicals and their metabolites in soils is often investigated in model matrices having undergone various pre-treatment steps that may…”
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    Journal Article
  4. 4

    Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic Bacterium Marinobacter sp. ELB17 by Chrast, Lukas, Tratsiak, Katsiaryna, Planas-Iglesias, Joan, Daniel, Lukas, Prudnikova, Tatyana, Brezovsky, Jan, Bednar, David, Kuta Smatanova, Ivana, Chaloupkova, Radka, Damborsky, Jiri

    Published in Microorganisms (Basel) (28-10-2019)
    “…Haloalkane dehalogenases are enzymes with a broad application potential in biocatalysis, bioremediation, biosensing and cell imaging. The new haloalkane…”
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    Journal Article
  5. 5

    Crystal structure of the novel haloalkane dehalogenases by Tratsiak, Katsiaryna, Prudnikova, Tatyana, Drienovska, Ivana, Chrast, Lukas, Damborsky, Jiri, Rezacova, Pavlina, Kuty, Michal, Chaloupkova, Radka, Kuta Smatanova, Ivana

    “…Abstract only Haloalkane dehalogenases (EC 3.8.1.5; HLDs) are microbial enzymes with catalytic activity for the hydrolytic conversion of xenobiotic and highly…”
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    Journal Article
  6. 6

    Computational Modelling of Metabolic Burden and Substrate Toxicity in Escherichia coli Carrying a Synthetic Metabolic Pathway by Demko, Martin, Chrást, Lukáš, Dvořák, Pavel, Damborský, Jiří, Šafránek, David

    Published in Microorganisms (Basel) (11-11-2019)
    “…In our previous work, we designed and implemented a synthetic metabolic pathway for 1,2,3-trichloropropane (TCP) biodegradation in . Significant effects of…”
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