Search Results - "Chrast, Lukas"
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Exacerbation of substrate toxicity by IPTG in Escherichia coli BL21(DE3) carrying a synthetic metabolic pathway
Published in Microbial cell factories (21-12-2015)“…Heterologous expression systems based on promoters inducible with isopropyl-β-D-1-thiogalactopyranoside (IPTG), e.g., Escherichia coli BL21(DE3) and cognate…”
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Gram-scale production of recombinant microbial enzymes in shake flasks
Published in FEMS microbiology letters (01-02-2018)“…Heterologous production of recombinant proteins is a cornerstone of microbiological and biochemical research as well as various biotechnological processes…”
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Influence of soil γ-irradiation and spiking on sorption of p,p′-DDE and soil organic matter chemistry
Published in Ecotoxicology and environmental safety (15-07-2018)“…The fate of organic chemicals and their metabolites in soils is often investigated in model matrices having undergone various pre-treatment steps that may…”
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Deciphering the Structural Basis of High Thermostability of Dehalogenase from Psychrophilic Bacterium Marinobacter sp. ELB17
Published in Microorganisms (Basel) (28-10-2019)“…Haloalkane dehalogenases are enzymes with a broad application potential in biocatalysis, bioremediation, biosensing and cell imaging. The new haloalkane…”
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Crystal structure of the novel haloalkane dehalogenases
Published in Acta crystallographica. Section A, Foundations and advances (05-08-2014)“…Abstract only Haloalkane dehalogenases (EC 3.8.1.5; HLDs) are microbial enzymes with catalytic activity for the hydrolytic conversion of xenobiotic and highly…”
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Computational Modelling of Metabolic Burden and Substrate Toxicity in Escherichia coli Carrying a Synthetic Metabolic Pathway
Published in Microorganisms (Basel) (11-11-2019)“…In our previous work, we designed and implemented a synthetic metabolic pathway for 1,2,3-trichloropropane (TCP) biodegradation in . Significant effects of…”
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Crystallographic analysis of new psychrophilic haloalkane dehalogenases: DpcA from Psychrobacter cryohalolentis K5 and DmxA from Marinobacter sp. ELB17
Published in Acta crystallographica. Section F, Structural biology and crystallization communications (01-06-2013)“…Haloalkane dehalogenases are hydrolytic enzymes with a broad range of potential practical applications such as biodegradation, biosensing, biocatalysis and…”
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Crystal structure of haloalkane dehalogenase DpcA from psychrophilic Psychrobacter cryohalolentis K5
Published in Acta crystallographica. Section A, Foundations of crystallography (25-08-2013)“…Abstract only…”
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