Genotyping of Brucella strains isolated from humans and cattle of different geographical regions of Pakistan using MLVA‐15

Genotyping of Brucella strains isolated from humans and cattle of different geographical regions of Pakistan using MLVA‐15

Background Prevalence of brucellosis and MLVA genotyping in animals and humans, isolated from different regions of Pakistan was performed. Animals having history of brucellosis from the field and local farms of Bannu, Mardan, Peshawar, Swat, Lahore and Islamabad were selected for blood collection. H...

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Published in:Veterinary medicine and science Vol. 7; no. 5; pp. 1688 - 1695
Main Authors: Akhtar, Raheela, Ali, Muhammad Muddassir, Ullah, Asad, Muttalib, Abdul, Mehboob, Kashan, Ullah, Arif, Ahmad, Nasim, Chohan, Tahir Zahoor
Format: Journal Article
Language:English
Published: England John Wiley & Sons, Inc 01-09-2021
John Wiley and Sons Inc
Wiley
Subjects:
Abortion
Animals
biovars
Brucella
Brucella melitensis - genetics
Brucellosis
Cattle
Farms
Genotype
Genotypes
Genotyping
Humans
Laboratories
Minisatellite Repeats
MLVA
Multilocus Sequence Typing - veterinary
Original
Pakistan
Pakistan - epidemiology
Phylogeny
Strains (organisms)
zoonotic
Pakistan
Islamabad Pakistan
India
Pakistan
zoonotic
biovars
MLVA
Brucella
genotyping
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Summary:Background Prevalence of brucellosis and MLVA genotyping in animals and humans, isolated from different regions of Pakistan was performed. Animals having history of brucellosis from the field and local farms of Bannu, Mardan, Peshawar, Swat, Lahore and Islamabad were selected for blood collection. Humans that work with them were also selected for sampling in this study. Total of 600 samples were taken from cattle and humans and subjected to Rose Bengal plate Test (RBPT) for the initial screening of positive samples. Designed primers of B.abortus for cattle and B.melitensis for humans were utilised to perform PCR. Culturing and isolation was carried to further to perform MLVA genotyping assay through the selection of two panels of primer markers. Results RBPT showed more number of cases of brucellosis in animals and humans compared to the PCR findings. Genotyping findings based upon MLVA‐15 set of markers demonstrated that the isolated strains of B.abortus fall in the same clade with strain A1, P8 and A2 from Pakistan and also similar with BCCN#02‐45 strain from India. On the other hand, B.melitensis isolated from different districts of Pakistan shared the same clade with BwIM‐AFG 63, BwIM‐AFG 44 strains from Afghanistan and BwIM IRN 37 strain from Iran. Selected VNTR alleles were sequenced for calibration purposes. Conclusion It is concluded that Brucella is prevalent in animals and humans in studied districts of Pakistan. Moreover, A1, P8, BwIM‐AFG 63, BwIM‐AFG 44 and A2 were found the common genotypes in Pakistan. Biovars; zoonotic; MLVA; Pakistan; Brucella; genotyping
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ISSN:2053-1095
2053-1095
DOI:10.1002/vms3.550