Characterization of Circulating HCV Genotypes: A Cohort Study in Uttar Pradesh, India

Hepatitis C virus infection is one of the main causes of liver disease that can lead to liver cirrhosis and finally to the chronic development of Hepatocellular Carcinoma (HCC). With 8 different HCV genotypes circulating, it becomes necessary to characterize the pathogenesis caused by each one of th...

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Bibliographic Details
Published in:	Journal of pure & applied microbiology : an international research journal of microbiology Vol. 18; no. 2; pp. 1057 - 1063
Main Authors:	Peer, Nuzhat Maqbool, Joshi, Vinod, Angel, Annette, Angel, Bennet, Chauhan, Vikram Singh, Buvvaji, Shareef Mohammed, Khaneja, Poorna, Khan, Ambreen Shafaat, Barthwal, Shilpa, Singh, Neha, Sharma, Bhawna, Joshi, Ramesh, Dheer, Monika, Kumari, Khushbu, Chitransh, Aarya, Chauhan, Reshu
Format:	Journal Article
Language:	English
Published:	Oriental Scientific Publishing Company 01-06-2024 Journal of Pure and Applied Microbiology
Subjects:	Analysis Development and progression Genetic aspects Genotype genotypes Health aspects Hepatitis C virus hepatocellular carcinoma india Infection Liver Liver cirrhosis Medical research Medicine, Experimental Viral proteins India
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Summary:	Hepatitis C virus infection is one of the main causes of liver disease that can lead to liver cirrhosis and finally to the chronic development of Hepatocellular Carcinoma (HCC). With 8 different HCV genotypes circulating, it becomes necessary to characterize the pathogenesis caused by each one of the HCV genotypes infecting population of a particular setting. Present study aims to identify the circulating Hepatitis C virus (HCV) genotypes in the different areas of State of Uttar Pradesh, India and understand their clinical etiology and related co-morbidities. The patients reporting to Sharda Hospital, Uttar Pradesh, India who were diagnosed positive for HCV were included in the study. The informed consent was obtained and then blood was drawn. In-house primers, for all the 8 genotypes were prepared and RT-PCR was performed. The amplified product was subjected to Agarose gel electrophoresis to identify the genotypes present in individual samples. Total 30 serum samples were taken. Of these, 21 (70%) showed presence of multiple genotypes, while 7 (23.33%) showed positivity for single genotype and two did not show any bands. Presence of multiple genotypes in individual patient has been reported for the first time. The pathological consequences of multiple genotypes within one host need to be studied further in terms of increased risk of developing a chronic liver disease. Further studies are being undertaken to study association of specific HCV genotype and multiple genotypes, Core, NS3 and NS5 viral proteins with the disease progression to develop predictors of liver cirrhosis and HCC.
ISSN:	0973-7510 2581-690X
DOI:	10.22207/JPAM.18.2.21