SLC17A3 rs9379800 and Ischemic Stroke Susceptibility at the Northern Region of Malaysia

SLC17A3 rs9379800 and Ischemic Stroke Susceptibility at the Northern Region of Malaysia

The relationships of Paired Like Homeodomain 2 (PITX2), Ninjurin 2 (NINJ2), TWIST-Related Protein 1 (TWIST1), Ras Interacting Protein 1 (Rasip1), Solute Carrier Family 17 Member 3 (SLC17A3), Methylmalonyl Co-A Mutase (MUT) and Fer3 Like BHLH Transcription Factor (FERD3L) polymorphisms and gene expre...

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Bibliographic Details
Published in:Journal of stroke and cerebrovascular diseases Vol. 30; no. 10; p. 105908
Main Authors: Ching, Shu Chai, Wen, Lim Jing, Ismail, Nor Ismaliza Mohd, Looi, Irene, Kooi, Cheah Wee, Peng, Long Soo, Mui, Lee Soon, Tamibmaniam, Jayashamani, Muninathan, Prema, Hooi, Ong Beng, Ali, Siti Maisarah Md, Hassan, Muhammad Radzi Abu, Mohamad, Mohd Saberi, Griffiths, Lyn R, Wei, Loo Keat
Format: Journal Article
Language:English
Published: Elsevier Inc 01-10-2021
Subjects:
Expression
Ischemic stroke
Malaysia
Polymorphism
SLC17A3
SNP
OR
GADPH
TWIST1
Malaysia
Ischemic stroke
PCR-RFLP
MUT
FERD3L
SNP
Expression
FDR
NINJ2
RT-PCR
HWE
SNPs
MDR
HPRT1
PITX2
Rasip1
95% CI
SLC17A3
US
Polymorphism
CART
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Summary:The relationships of Paired Like Homeodomain 2 (PITX2), Ninjurin 2 (NINJ2), TWIST-Related Protein 1 (TWIST1), Ras Interacting Protein 1 (Rasip1), Solute Carrier Family 17 Member 3 (SLC17A3), Methylmalonyl Co-A Mutase (MUT) and Fer3 Like BHLH Transcription Factor (FERD3L) polymorphisms and gene expression with ischemic stroke have yet to be determined in Malaysia. Hence, this study aimed to explore the associations of single nucleotide polymorphisms (SNPs) and gene expression with ischemic stroke risk among population who resided at the Northern region of Malaysia. Study subjects including 216 ischemic stroke patients and 203 healthy controls were recruited upon obtaining ethical clearance. SNP genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism assays. Gene expression levels were quantified by real-time polymerase chain reaction assays. Statistical and genetic analyses were conducted with SPSS version 22.2, PLINK version 1.07 and multifactor dimensionality reduction software. Study subjects with G allele, CG or GG genotypes of SLC17A3 rs9379800 demonstrated increased risk of ischemic stroke with the odds ratios ranging from 1.76-fold to 3.14-fold (p<0.05). When stratified study subjects according to the ethnicity, SLC17A3 rs9379800 G allele and CG genotype contributed to 2.14- and 2.96-fold of ischemic stroke risk among Malay population significantly, in the multivariate analysis (p<0.05). However, no significant associations were observed for PITX2, NINJ2, TWIST1, Rasip1, and MUT polymorphisms with ischemic stroke risk in the multivariate analysis for the pooled cases and controls as well as when stratified them according to the ethnicity. Lower mRNA expression levels of Rasip1, SLC17A3, MUT and FERD3L were observed among cases (p<0.05). After FDR adjustment, the mRNA level of SLC17A3 remained significantly associated with ischemic stroke among Malay population (q=0.034). In conclusion, this study suggests that SLC17A3 rs9379800 polymorphism and its gene expression contribute to significant ischemic stroke risk among Malaysian population, particularly the Malay who resided at the Northern Region of the country. Our findings can provide useful information for the future diagnosis, management and treatment of ischemic stroke patients.
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ISSN:1052-3057
1532-8511
DOI:10.1016/j.jstrokecerebrovasdis.2021.105908