Increased endothelin-1 content in the platelets of hemodialysed patients

Patients undergoing hemodialysis, present platelets (PLTs) with physiological dysfunction. Aggregated PLTs stimulate endothelin-1 (ET-1) secretion from endothelial cells. In turn, ET-1 abolishes PLT aggregation. The aim of this study was to determine any presence of ET-1 in the PLTs of hemodialysed...

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Bibliographic Details
Published in:	Clinical nephrology Vol. 48; no. 3; p. 185
Main Authors:	Vlachojannis, J, Tsakas, S, Chinari, E, Orphanos, V, Zoumbos, N, Kurz, P
Format:	Journal Article
Language:	English
Published:	Germany 01-09-1997
Subjects:	Adult Blood Platelets - chemistry Blood Platelets - metabolism Blood Protein Electrophoresis Case-Control Studies Electrophoresis, Polyacrylamide Gel Endothelin-1 - blood Female Humans Kidney Failure, Chronic - blood Kidney Failure, Chronic - therapy Male Platelet Aggregation Polymerase Chain Reaction Renal Dialysis
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Summary:	Patients undergoing hemodialysis, present platelets (PLTs) with physiological dysfunction. Aggregated PLTs stimulate endothelin-1 (ET-1) secretion from endothelial cells. In turn, ET-1 abolishes PLT aggregation. The aim of this study was to determine any presence of ET-1 in the PLTs of hemodialysed patients and to compare its levels with normal subjects. Platelets, isolated from hemodialysed patients, revealed lower aggregation (76 +/- 13%) compared with healthy subjects (96 +/- 2%). Plasma ET-1 was increased in hemodialysed patients 23.5 +/- 3.2 fmol/ml, vs. 10.9 +/- 1.6 fmol/ml in normal subjects. Immunoreactive endothelin-1 was detected in the platelets of both groups. The intraplatelet ET-1 of hemodialysed patients was 13.8 +/- 3.1 fmol/mg protein, vs. 7.9 +/- 1.3 fmol/mg protein in normal individuals. Total RNA was isolated from platelets and reverse transcriptase-mediated polymerase chain reaction (RT-PCR) revealed no presence of the preproET-1 mRNA in either normal or hemodialysed platelets. This suggests that ET-1 is internalized from the plasma. In summary, platelets contain but do not express ET-1. The increased levels of ET-1 in the platelets of the hemodialysed patients may be partly responsible for their lower aggregation.
ISSN:	0301-0430