Klebsiella pneumoniae antibiotic resistance identified by atomic force microscopy

Klebsiella pneumoniae antibiotic resistance identified by atomic force microscopy

In the last decade the detection of the resistance of bacteria to antibiotics treatment, developed by different kind of bacteria, is becoming a huge problem. We hereby present a different approach to the current problem of detection of bacteria resistance to antibiotics. Our aims were to use the ato...

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Bibliographic Details
Published in:Journal of biosciences Vol. 42; no. 4; pp. 623 - 636
Main Authors: Ierardi, Vincenzo, Domenichini, Paolo, Reali, Silvia, Chiappara, Gian Marco, Devoto, Gianluigi, Valbusa, Ugo
Format: Journal Article
Language:English
Published: New Delhi Springer India 01-12-2017
Springer Nature B.V
Subjects:
Antibiotic resistance
Antibiotics
Atomic force microscopy
Bacteria
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell walls
Detection
Disease resistance
Drug resistance
Klebsiella
Klebsiella pneumoniae
Life Sciences
Microbiological strains
Microbiology
Microscopes
Microscopy
Morphology
Nuclear energy
Plant Sciences
Strains (organisms)
Zoology
AFM
Bacteria images
Antibiotic resistance
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Summary:In the last decade the detection of the resistance of bacteria to antibiotics treatment, developed by different kind of bacteria, is becoming a huge problem. We hereby present a different approach to the current problem of detection of bacteria resistance to antibiotics. Our aims were to use the atomic force microscopy (AFM) to investigate bacteria morphological changes in response to antibiotics treatment and explore the possibility of reducing the time required to obtain information on their resistance. In particular, we studied Klebsiella pneumoniae bacteria provided by the Lavagna Hospital ASL4 Liguria (Italy), where there are cases linked with antibiotics resistance of the Klebsiella pneumoniae . By comparing AFM images of bacteria strains treated with different antibiotics is possible to identify unambiguously the Klebsiella pneumoniae strains resistant to antibiotics. In fact, the analysis of the AFM images of the antibiotic-sensitive bacteria shows clearly the presence of morphological alterations in the cell wall. While in the case of the antibiotic-resistant bacteria morphological alterations are not present. This approach is based on an easy and potentially rapid AFM analysis.
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ISSN:0250-5991
0973-7138
DOI:10.1007/s12038-017-9713-6