Noscapine shows antimalarial activity against Plasmodium falciparum 3D7, its clinical isolate Pf140/SS, and Plasmodium berghei ANKA

Objective: To evaluate the antimalarial activity of noscapine against Plasmodium falciparum 3D7 strain (Pf3D7), its clinical isolate (Pf140/SS), and Plasmodium berghei ANKA (PbA). Methods: Using ring-stage survival assay, phenotypic assessments, and SYBR-green-based fluorescence assay, the antimalar...

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Published in:	Asian Pacific journal of tropical biomedicine Vol. 14; no. 8; pp. 350 - 358
Main Authors:	Babu, Swaraj Kumar, Maharana, Sameer, Chhatria, Satyaranjan, Sahoo, Dibya Ranjan, Nanda, Ashirbad, Kanhar, Satish, Behera, Prativa K., Mohanty, Sanjib, Naik, Pradeep Kumar, Sahu, Praveen Kishore
Format:	Journal Article
Language:	English
Published:	Wolters Kluwer Medknow Publications 01-08-2024
Subjects:	antimalarial cytotoxicity dihydroartemisinin falcipain-2 malaria noscapine plasmodium berghei plasmodium falciparum
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Summary:	Objective: To evaluate the antimalarial activity of noscapine against Plasmodium falciparum 3D7 strain (Pf3D7), its clinical isolate (Pf140/SS), and Plasmodium berghei ANKA (PbA). Methods: Using ring-stage survival assay, phenotypic assessments, and SYBR-green-based fluorescence assay, the antimalarial activities of noscapine were assessed compared with dihydroartemisinin (DHA) in in vivo and in vitro studies. In addition, hemolysis and cytotoxicity tests were carried out to evaluate its safety. RT-PCR assay was also conducted to determine the effect of noscapine on papain-like cysteine protease Plasmodium falciparum falcipain-2 (PfFP-2). Results: The antimalarial efficacy of noscapine against Pf3D7 and Pf140/SS was comparable to DHA, with IC50 values of (7.68±0.88) and (5.57±0.74) nM/mL, respectively, and >95% inhibition of PbA infected rats. Noscapine also showed a safe profile, as evidenced by low hemolysis and cytotoxicity even at high concentrations. Moreover, PfFP-2 expression was significantly inhibited in both noscapine-treated Pf3D7 and Pf140/SS (P<0.01). Conclusions: Noscapine has antimalarial properties comparable to standard antimalarial DHA with better safety profiles, which may be further explored as a therapeutic candidate for the treatment of malaria.
ISSN:	2221-1691 2588-9222
DOI:	10.4103/apjtb.apjtb_342_24