Search Results - "Chen, Lorenzo H."
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Structure of the Capsular Polysaccharide of Pneumococcal Serotype 11A Reveals a Novel Acetylglycerol That Is the Structural Basis for 11A Subtypes
Published in The Journal of biological chemistry (13-03-2009)“…We have undertaken a structural assessment of Streptococcus pneumoniae 11A polysaccharide as well as two clinical isolates related to 11A. The clinical…”
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Creatine Kinase: Essential Arginine Residues at the Nucleotide Binding Site Identified by Chemical Modification and High-Resolution Tandem Mass Spectrometry
Published in Proceedings of the National Academy of Sciences - PNAS (31-03-1998)“…Phenylglyoxal is an arginine-specific reagent that inactivates creatine kinase (CK). Previous results suggest that modification of the dimeric enzyme at a…”
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A reversed phase HPLC assay for the simultaneous quantitation of non-ionic and ionic surfactants in bioprocess intermediates
Published in Journal of pharmaceutical and biomedical analysis (03-03-2006)“…This report describes a rapid and accurate reversed phase HPLC method for the simultaneous quantitation of multiple surfactants in various bioprocess solution…”
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Catalytic Role of the Amino-Terminal Proline in 4-Oxalocrotonate Tautomerase: Affinity Labeling and Heteronuclear NMR Studies
Published in Biochemistry (Easton) (23-01-1996)“…4-Oxalocrotonate tautomerase (EC 5.3.2-; 4-OT), a hexamer consisting of 62 residues per subunit, catalyzes the isomerization of unsaturated α-keto acids,…”
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Rabbit Muscle Creatine Kinase: Consequences of the Mutagenesis of Conserved Histidine Residues
Published in Biochemistry (Easton) (18-06-1996)“…Creatine kinase (CK; EC 2.7.3.2) catalyzes the reversible conversion of creatine and MgATP to phosphocreatine and MgADP. In the absence of an X-ray crystal…”
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4-Oxalocrotonate tautomerase, an enzyme composed of 62 amino acid residues per monomer
Published in The Journal of biological chemistry (05-09-1992)“…The xylH gene encoding 4-oxalocrotonate tautomerase (4-OT) has been located on a subclone of the Pseudomonas putida mt-2 TOL plasmid pWW0 and inserted into an…”
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Direct Sequence Data from Heterogeneous Creatine Kinase (43 kDa) by High-Resolution Tandem Mass Spectrometry
Published in Biochemistry (Easton) (19-12-1995)“…Isoelectric focusing separation of recombinant rabbit muscle creatine kinase (CK) and its 282Cys-->282Ser mutant shows the presence of three and two isoforms,…”
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Sequence Verification of Human Creatine Kinase (43 kDa) Isozymes by High- Resolution Tandem Mass Spectrometry
Published in Proceedings of the National Academy of Sciences - PNAS (05-12-1995)“…Amino acid sequencing by recombinant DNA technology, although dramatically useful, is subject to base reading errors, is indirect, and is insensitive to…”
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A comparative study of human muscle and brain creatine kinases expressed in Escherichia coli
Published in Journal of Protein Chemistry (01-01-2000)“…We report the expression of the human muscle (CK-MM) and brain (CK-BB) creatine kinases in Escherichia coli. The proteins have been purified to apparent…”
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Random and site-directed mutagenesis of bacterial luciferase: investigation of the aldehyde binding site
Published in Biochemistry (Easton) (21-03-1989)“…Numerous luciferase structural gene mutants of Vibrio harveyi have been generated by random mutagenesis and phenotypically characterized [Cline, T.W., &…”
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Structure of the Capsular Polysaccharide of Pneumococcal Serotype 11A Reveals a Novel Acetylglycerol That Is the Structural Basis for 11A SubtypesS
Published in The Journal of biological chemistry (13-03-2009)“…We have undertaken a structural assessment of Streptococcus pneumoniae 11A polysaccharide as well as two clinical isolates related to 11A. The clinical…”
Get full text
Journal Article -
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Cloning and expression of functional rabbit muscle creatine kinase in Escherichia coli. Addressing the problem of microheterogeneity
Published in The Journal of biological chemistry (25-06-1991)“…The gene encoding rabbit muscle creatine kinase (CK) has been subcloned into a single plasmid, and the protein expressed in a soluble and functional form in…”
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