Search Results - "Chen, Bifan"

Nanoproteomics enables proteoform-resolved analysis of low-abundance proteins in human serum by Tiambeng, Timothy N., Roberts, David S., Brown, Kyle A., Zhu, Yanlong, Chen, Bifan, Wu, Zhijie, Mitchell, Stanford D., Guardado-Alvarez, Tania M., Jin, Song, Ge, Ying

“…Top-down mass spectrometry (MS)-based proteomics provides a comprehensive analysis of proteoforms to achieve a proteome-wide understanding of protein…”
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A photocleavable surfactant for top-down proteomics by Brown, Kyle A., Chen, Bifan, Guardado-Alvarez, Tania M., Lin, Ziqing, Hwang, Leekyoung, Ayaz-Guner, Serife, Jin, Song, Ge, Ying

“…We report the identification of a photocleavable anionic surfactant, 4-hexylphenylazosulfonate (Azo), which can be rapidly degraded by ultraviolet irradiation,…”
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Top-Down Proteomics of Large Proteins up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy by Cai, Wenxuan, Tucholski, Trisha, Chen, Bifan, Alpert, Andrew J, McIlwain, Sean, Kohmoto, Takushi, Jin, Song, Ge, Ying

“…Mass spectrometry (MS)-based top-down proteomics is a powerful method for the comprehensive analysis of proteoforms that arise from genetic variations and…”
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Online Hydrophobic Interaction Chromatography–Mass Spectrometry for the Analysis of Intact Monoclonal Antibodies by Chen, Bifan, Lin, Ziqing, Alpert, Andrew J, Fu, Cexiong, Zhang, Qunying, Pritts, Wayne A, Ge, Ying

“…Therapeutic monoclonal antibodies (mAbs) are an important class of drugs for a wide spectrum of human diseases. Liquid chromatography (LC) coupled to mass…”
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Reproducible large-scale synthesis of surface silanized nanoparticles as an enabling nanoproteomics platform: Enrichment of the human heart phosphoproteome by Roberts, David S., Chen, Bifan, Tiambeng, Timothy N., Wu, Zhijie, Ge, Ying, Jin, Song

“…A reproducible synthetic strategy was developed for facile large-scale (200 mg) synthesis of surface silanized magnetite (Fe 3 O 4 ) nanoparticles (NPs) for…”
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Impact of Phosphorylation on the Mass Spectrometry Quantification of Intact Phosphoproteins by Wu, Zhijie, Tiambeng, Timothy N, Cai, Wenxuan, Chen, Bifan, Lin, Ziqing, Gregorich, Zachery R, Ge, Ying

“…Protein phosphorylation is a ubiquitous and critical post-translational modification (PTM) involved in numerous cellular processes. Mass spectrometry…”
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Top-Down Proteomics: Ready for Prime Time? by Chen, Bifan, Brown, Kyle A, Lin, Ziqing, Ge, Ying

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Characterization of synthetic guide ribonucleic acids through hydrophilic interaction chromatography coupled with mass spectrometry by Wei, Bingchuan, Wang, Jenny, Dai, Lulu, Chen, Bifan, Zhang, Kelly

“…•Generic HILIC method was developed for gRNA analysis with mechanism revealed.•Suppressing surface charge is critical on HILIC separation of large…”
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Top-Down Mass Spectrometry of Synthetic Single Guide Ribonucleic Acids Enabled by Facile Sample Clean-Up by Crittenden, Christopher M., Lanzillotti, Michael B., Chen, Bifan

“…In recent years, CRISPR-Cas9 genome editing has become an important technology in biomedical research and has demonstrated tremendous therapeutic potential…”
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Development of an ion pairing reversed-phase liquid chromatography-mass spectrometry method for characterization of clustered regularly interspaced short palindromic repeats guide ribonucleic acid by Wei, Bingchuan, Wang, Jenny, Cadang, Lance, Goyon, Alexandre, Chen, Bifan, Yang, Feng, Zhang, Kelly

“…•A generic stability indicating IP-RPLC method for gRNA analysis was developed.•Wide pore size column is required for the 100-mer gRNA separation.•Charge…”
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Online Hydrophobic Interaction Chromatography–Mass Spectrometry for Top-Down Proteomics by Chen, Bifan, Peng, Ying, Valeja, Santosh G, Xiu, Lichen, Alpert, Andrew J, Ge, Ying

“…Recent progress in top-down proteomics has led to a demand for mass spectrometry (MS)-compatible chromatography techniques to separate intact proteins using…”
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A Top-Down Proteomics Platform Coupling Serial Size Exclusion Chromatography and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry by Tucholski, Trisha, Knott, Samantha J, Chen, Bifan, Pistono, Paige, Lin, Ziqing, Ge, Ying

“…Mass spectrometry (MS) based top-down proteomics provides rich information about proteoforms arising from combinatorial amino acid sequence variations and…”
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Best practices and benchmarks for intact protein analysis for top-down mass spectrometry by Donnelly, Daniel P., Rawlins, Catherine M., DeHart, Caroline J., Fornelli, Luca, Schachner, Luis F., Lin, Ziqing, Lippens, Jennifer L., Aluri, Krishna C., Sarin, Richa, Chen, Bifan, Lantz, Carter, Jung, Wonhyeuk, Johnson, Kendall R., Koller, Antonius, Wolff, Jeremy J., Campuzano, Iain D. G., Auclair, Jared R., Ivanov, Alexander R., Whitelegge, Julian P., Paša-Tolić, Ljiljana, Chamot-Rooke, Julia, Danis, Paul O., Smith, Lloyd M., Tsybin, Yury O., Loo, Joseph A., Ge, Ying, Kelleher, Neil L., Agar, Jeffrey N.

“…One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the…”
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Characterization of High Molecular Weight Multi-Arm Functionalized PEG-Maleimide for Protein Conjugation by Charge-Reduction Mass Spectrometry Coupled to Two-Dimensional Liquid Chromatography by Yang, Samuel H, Chen, Bifan, Wang, Jenny, Zhang, Kelly

“…A current trend in drug development involves the use of high molecular weight, branched, and functionalized polymers for protein conjugation and drug delivery…”
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Ultraviolet photodissociation facilitates mass spectrometry-based structure elucidation with pyrrolidine and piperidine containing compounds by Chen, Bifan, Kurita, Kenji L., Wong, Nicholas, Crittenden, Christopher M.

“…In pharmaceutical development, structural elucidation of small molecules from process related impurities and degradation products is an essential component. As…”
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Middle-Down Multi-Attribute Analysis of Antibody-Drug Conjugates with Electron Transfer Dissociation by Chen, Bifan, Lin, Ziqing, Zhu, Yanlong, Jin, Yutong, Larson, Eli, Xu, Qingge, Fu, Cexiong, Zhang, Zhaorui, Zhang, Qunying, Pritts, Wayne A, Ge, Ying

“…Antibody-drug conjugates (ADCs) are designed to combine the target specificity of monoclonal antibodies and potent cytotoxin drugs to achieve better…”
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Streamlining LC-MS Characterization of Pharmaceutical Polymers by Fourier-Transform-Based Deconvolution and Macromolecular Mass Defect Analysis by Swansiger, Andrew K., Crittenden, Christopher M., Chan, Simon A., Yang, Samuel H., Kou, Dawen, Prell, James S., Chen, Bifan

“…Polymer conjugation has risen in importance over the past three decades as a means of increasing the in vivo half-life of biotherapeutics, with benefits…”
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Rapid Analysis of Reduced Antibody Drug Conjugate by Online LC-MS/MS with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry by Larson, Eli J, Zhu, Yanlong, Wu, Zhijie, Chen, Bifan, Zhang, Zhaorui, Zhou, Shiyue, Han, Linjie, Zhang, Qunying, Ge, Ying

“…Antibody drug conjugates (ADCs), which harness the high targeting specificity of monoclonal antibodies (mAb) with the potency of small molecule therapeutics,…”
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Correction to Top-Down Proteomics: Ready for Prime Time? by Chen, Bifan, Brown, Kyle A, Lin, Ziqing, Ge, Ying

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Coupling functionalized cobalt ferrite nanoparticle enrichment with online LC/MS/MS for top-down phosphoproteomics by Chen, Bifan, Hwang, Leekyoung, Ochowicz, William, Lin, Ziqing, Guardado-Alvarez, Tania M, Cai, Wenxuan, Xiu, Lichen, Dani, Kunal, Colah, Cyrus, Jin, Song, Ge, Ying

“…Phosphorylation plays pivotal roles in cellular processes and dysregulated phosphorylation is considered as an underlying mechanism in many human diseases…”
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